دورية أكاديمية
Genome-wide strategies identify downstream target genes of chick connective tissue-associated transcription factors
| العنوان: | Genome-wide strategies identify downstream target genes of chick connective tissue-associated transcription factors |
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| المؤلفون: | Orgeur, Mickael, Martens, Marvin, Leonte, Georgeta, Nassari, Sonya, Bonnin, Marie-Ange, Börno, Stefan T., Timmermann, Bernd, Hecht, Jochen, Duprez, Delphine, Stricker, Sigmar |
| بيانات النشر: | The Company of Biologists Ltd |
| سنة النشر: | 2018 |
| المجموعة: | HighWire Press (Stanford University) |
| مصطلحات موضوعية: | TECHNIQUES AND RESOURCES |
| الوصف: | Connective tissues support organs and play crucial roles in development, homeostasis and fibrosis, yet our understanding of their formation is still limited. To gain insight into the molecular mechanisms of connective tissue specification, we selected five zinc-finger transcription factors – OSR1, OSR2, EGR1, KLF2 and KLF4 – based on their expression patterns and/or known involvement in connective tissue subtype differentiation. RNA-seq and ChIP-seq profiling of chick limb micromass cultures revealed a set of common genes regulated by all five transcription factors, which we describe as a connective tissue core expression set. This common core was enriched with genes associated with axon guidance and myofibroblast signature, including fibrosis-related genes. In addition, each transcription factor regulated a specific set of signalling molecules and extracellular matrix components. This suggests a concept whereby local molecular niches can be created by the expression of specific transcription factors impinging on the specification of local microenvironments. The regulatory network established here identifies common and distinct molecular signatures of limb connective tissue subtypes, provides novel insight into the signalling pathways governing connective tissue specification, and serves as a resource for connective tissue development. |
| نوع الوثيقة: | text |
| وصف الملف: | text/html |
| اللغة: | English |
| العلاقة: | http://dev.biologists.org/cgi/content/short/145/7/dev161208Test; http://dx.doi.org/10.1242/dev.161208Test |
| DOI: | 10.1242/dev.161208 |
| الإتاحة: | https://doi.org/10.1242/dev.161208Test http://dev.biologists.org/cgi/content/short/145/7/dev161208Test |
| حقوق: | Copyright (C) 2018, Company of Biologists |
| رقم الانضمام: | edsbas.4292E23 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1242/dev.161208 |
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