دورية أكاديمية
A novel method for determination of the (15) N isotopic composition of Rubisco in wheat plants exposed to elevated atmospheric carbon dioxide.
| العنوان: | A novel method for determination of the (15) N isotopic composition of Rubisco in wheat plants exposed to elevated atmospheric carbon dioxide. |
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| المؤلفون: | Aranjuelo, Iker, Molero, Gemma, Avice, Jean Christophe, Bourguignon, Jacques |
| المساهمون: | Department of Plant Biology and Ecology (Bilbao, Spain), Universidad del Pais Vasco / Euskal Herriko Unibertsitatea Espagne (UPV/EHU), Laboratoire de physiologie cellulaire végétale (LPCV), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Recherche Agronomique (INRA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), International Maize and Wheat Improvement Center (CIMMYT), Consultative Group on International Agricultural Research CGIAR (CGIAR), Ecophysiologie Végétale, Agronomie et Nutritions (EVA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Recherche Agronomique (INRA), Spanish National Research and Development Programme (AGL2011-30386-C02-2)- European Molecular Biology Organization (EMBO) |
| المصدر: | ISSN: 0031-9317. |
| بيانات النشر: | HAL CCSD Wiley |
| سنة النشر: | 2015 |
| المجموعة: | HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
| مصطلحات موضوعية: | Nitrogen partitioning, Photosynthesis, Plant, Wheat, Triticum durum, 5- bisphosphate carboxylase, Enzyme, Acclimation, Environmental changes, Calvin cycle, Ribulose-1, Radiolabeling, Purification protocol, Yield, Carbon dioxide elevation, [SDV]Life Sciences [q-bio], [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology |
| الوصف: | International audience ; Although ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) is mostly known as a key enzyme involved in CO2 assimilation during the Calvin cycle, comparatively little is known about its role as a pool of nitrogen storage in leaves. For this purpose, we developed a protocol to purify Rubisco that enables later analysis of its (15) N isotope composition (δ(15) N) at the natural abundance and (15) N-labeled plants. In order to test the utility of this protocol, durum wheat (Triticum durum var. Sula) exposed to an elevated CO2 concentration (700 vs 400 µmol mol(-1) ) was labeled with K(15) NO3 (enriched at 2 atom %) during the ear development period. The developed protocol proves to be selective, simple, cost effective and reproducible. The study reveals that (15) N labeling was different in total organic matter, total soluble protein and the Rubisco fraction. The obtained data suggest that photosynthetic acclimation in wheat is caused by Rubisco depletion. This depletion may be linked to preferential nitrogen remobilization from Rubisco toward grain filling. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/25272325; hal-01143256; https://hal.science/hal-01143256Test; PRODINRA: 312758; PUBMED: 25272325; WOS: 000348722400001 |
| DOI: | 10.1111/ppl.12294 |
| الإتاحة: | https://doi.org/10.1111/ppl.12294Test https://hal.science/hal-01143256Test |
| رقم الانضمام: | edsbas.63E6984A |
| قاعدة البيانات: | BASE |
| DOI: | 10.1111/ppl.12294 |
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