دورية أكاديمية
A global benchmark study using affinity-based biosensors
| العنوان: | A global benchmark study using affinity-based biosensors |
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| المؤلفون: | Rich, Rebecca L., Papalia, Giuseppe A., Flynn, Peter J., Furneisen, Jamie, Quinn, John, Klein, Joshua S., Katsamba, Phini S., Waddell, M. Brent, Scott, Michael, Thompson, Joshua, Berlier, Judie, Corry, Schuyler, Baltzinger, Mireille, Zeder-Lutzi, Gabrielle, Schoenemann, Andreas, Clabbers, Anca, Wieckowski, Sébastien, Murphy, Mary M., Page, Phillip, Ryan, Thomas E., Duffner, Jay, Ganguly, Tanmoy, Corbin, John, Gautam, Satyen, Anderluh, Gregor, Bavdek, Andrej, Reichmann, Dana, Yadav, Satya P., Hommema, Eric, Pol, Ewa, Drake, Andrew, Klakamp, Scott, Chapman, Trevor, Kernaghan, Dawn, Miller, Ken, Schuman, Jason, Lindquist, Kevin, Herlihy, Kara, Murphy, Michael B., Bohnsack, Richard, Andrien, Bruce, Brandani, Pietro, Terwey, Danny, Millican, Rohn, Darling, Ryan J., Wang, Liann, Carter, Quincy, Dotzlaf, Joe, Lopez-Sagaseta, Jacinto, Campbell, Islay, Torreri, Paola, Hoos, Sylviane, England, Patrick, Liu, Yang, Abdiche, Yasmina, Malashock, Daniel, Pinkerton, Alanna, Wong, Melanie, Lafer, Eileen, Hinck, Cynthia, Thompson, Kevin, Di Primo, Carmelo, Joyce, Alison, Brooks, Jonathan, Torta, Federico, Hagel, Anne Birgitte Bagge, Krarup, Janus, Pass, Jesper, Ferreira, Monica, Shikov, Sergei, Mikolajczyk, Malgorzata, Abe, Yuki, Barbato, Gaetano, Giannetti, Anthony M., Krishnamoorthy, Ganeshram, Beusink, Bianca, Satpaev, Daulet, Tsang, Tiffany, Fang, Eric, Partridge, James, Brohawn, Stephen, Horn, James, Pritsch, Otto, Obal, Gonzalo, Nilapwar, Sanjay, Busby, Ben, Gutierrez-Sanchez, Gerardo, das Gupta, Ruchira, Canepa, Sylvie, Witte, Krista, Nikolovska-Coleska, Zaneta, Cho, Yun Hee, d'Agata, Roberta, Schlick, Kristian, Calvert, Rosy, Munoz, Eva M., Hernaiz, Maria Jose, Bravman, Tsafir, Dines, Monica, Yang, Min-Hsiang, Puskas, Agnes, Boni, Erica, Li, Jiejin, Wear, Martin, Grinberg, Asya, Baardsnes, Jason, Dolezal, Olan, Gainey, Melicia, Anderson, Henrik, Peng, Jinlin, Lewis, Mark, Spies, Peter, Trinh, Quyhn, Bibikov, Sergei, Raymond, Jill, Yousef, Mohammed, Chandrasekaran, Vidya, Feng, Yuguo, Emerick, Anne, Mundodo, Suparna, Guimaraes, Rejane, Mcgirr, Katy, Li, Yue-Ji, Hughes, Heather, Mantz, Hubert, Skrabana, Rostislav, Witmer, Mark, Ballard, Joshua, Martin, Loïc, M, Skladal, Petr, Korza, George, Laird-Offringa, Ite, Lee, Charlene S., Khadir, Abdelkrim, Podlaski, Frank, Neuner, Phillippe, Rothacker, Julie, Rafique, Ashique, Dankbar, Nico, Kainz, Peter, Gedig, Erk, Vuyisich, Momchilo, Boozer, Christina, Ly, Nguyen, Toews, Mark, Uren, Aykut, Kalyuzhniy, Oleksandr, Lewis, Kenneth, Chomey, Eugene, Pak, Brian J., Myszka, David G. |
| المساهمون: | School of Medicine University of Utah, Salt Lake City, University of Utah, KaloBios Pharmaceuticals San Francisco, Schering–Plough Biopharma Palo Alto, Nomadics Oklahoma City, Biochemistry and Molecular Biophysics Pasadena, California Institute of Technology (CALTECH), Department of Biochemistry and Molecular Biophysics, Columbia University New York, Hartwell Center for Bioinformatics and Biotechnology Memphis, St Jude Children's Research Hospital, Universität Zürich Zürich = University of Zurich (UZH), Molecular Probes/Invitrogen Eugene, Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), MerckKGaA, Merck Sharp and Dohme (Merck & Co. Inc), Abbott Bioresearch Center Worcester, Institut de biologie cellulaire et moléculaire Strasbourg, Université de Strasbourg (UNISTRA), Reichert Depew}, Momenta Pharmaceuticals Cambridge, XOMA (U.S.), Department of Chemical and Biomolecular Engineering, National University of Singapore, National University of Singapore (NUS), Department of Biology Ljubljiana, University of Ljubljana, Department of Biological Chemistry (Weizmann Institute of Science), Weizmann Institute of Science Rehovot, Israël, Molecular Biotechnology Core Laboratory Cleveland, Cleveland Clinic Foundation, ThermoFisher Scientific Rockford, Biacore/GE Healthcare Uppsala, AstraZeneca Hayward, Neurodegeneration Research Department Harlow, GlaxoSmithKline Harlow, MedImmune, Biacore/GE Healthcare Piscataway, Department of Biochemistry Milwaukee, Medical College of Wisconsin Milwaukee (MCW), Alexion Pharmaceuticals Cheshire, Applied Biosystems Foster City, diaDexus South San Francisco, Eli Lilly and Company Indianapolis, Center for Applied Medical Research Plamplona (CIMA), Universidad de Navarra Pamplona (UNAV), EMD Lexigen Research Center Billerica, Department of Cell Biology and Neuroscience Rome, Istituto Superiore di Sanità = National Institute of Health (ISS), Département de Biologie structurale et Chimie - Department of Structural Biology and Chemistry, Institut Pasteur Paris (IP), Department of Chemistry Atlanta, Georgia State University, University System of Georgia (USG)-University System of Georgia (USG), Pfizer/Rinat Laboratories South San Francisco, PDLBioPharma Fremont, Department of Biochemistry San Antonio, University of Texas Health Science Center at San Antonio San Antonio, Tx, USA, Akubio Cambridge, ARN : régulations naturelle et artificielle, Université Bordeaux Segalen - Bordeaux 2-Institut Européen de Chimie et de Biologie-Institut National de la Santé et de la Recherche Médicale (INSERM), Wyeth Research Cambridge, Department of Biochemistry and Molecular Biology Odense, University of Southern Denmark (SDU), NovoNordisk Gentofte, Södertörns University College Huddinge, Department of Biochemistry Philadelphia, Temple University Philadelphia, Pennsylvania Commonwealth System of Higher Education (PCSHE)-Pennsylvania Commonwealth System of Higher Education (PCSHE), U.S. Food and Drug Administration (FDA), Department of Biochemical Engineering London, University College of London London (UCL), Merck Rome, Roche Palo Alto, University of Twente, Agensys Santa Monica, Novartis Emeryville, Massachusetts Institute of Technology (MIT), Northern Illinois University, Institut Pasteur de Montevideo, Réseau International des Instituts Pasteur (RIIP), University of Manchester Manchester, Department of Physiology Baltimore, University of Maryland Baltimore, Complex Carbohydrate Research Center Athens, GA, USA, University of Georgia USA, Adnexus Therapeutics Waltham}, Physiologie de la reproduction et des comportements Nouzilly (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation Saumur (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ForteBio Menlo Park, University of Michigan Ann Arbor, University of Michigan System, Human Genome Sciences Rockville, Department of Chemical Sciences Catania, Università degli studi di Catania = University of Catania (Unict), Montana State University (MSU), GKT School of Biomedical Sciences London, Organic and Pharmaceutical Chemistry Department Madrid, Universidad Complutense de Madrid = Complutense University of Madrid Madrid (UCM), Bio-Rad Haifa |
| المصدر: | ISSN: 0003-2697. |
| بيانات النشر: | HAL CCSD Elsevier Masson |
| سنة النشر: | 2009 |
| المجموعة: | HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
| مصطلحات موضوعية: | Kinetics, Biacore, Optical biosensor, Surface plasmon resonance, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| الوصف: | International audience ; To explore the variability in biosensor studies, 150 participants from 20 countries were given the same protein samples and asked to determine kinetic rate constants for the interaction. We chose a protein system that was amenable to analysis using different biosensor platforms as well as by users of different expertise levels. The two proteins (a 50-kDa Fab and a 60-kDa glutathione S-transferase [GST] antigen) form a relatively high-affinity complex, so participants needed to optimize several experimental parameters, including ligand immobilization and regeneration conditions as well as analyte concentrations and injection/dissociation times. Although most participants collected binding responses that could be fit to yield kinetic parameters, the quality of a few data sets could have been improved by optimizing the assay design. Once these outliers were removed, the average reported affinity across the remaining panel of participants was 620 pM with a standard deviation of 980 pM. These results demonstrate that when this biosensor assay was designed and executed appropriately, the reported rate constants were consistent, and independent of which protein was immobilized and which biosensor was used. (C) 2008 Elsevier Inc. All rights reserved. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/19133223; hal-01527917; https://hal.science/hal-01527917Test; https://hal.science/hal-01527917/documentTest; https://hal.science/hal-01527917/file/nihms500790.pdfTest; PRODINRA: 27332; PUBMED: 19133223; WOS: 000263789500009 |
| DOI: | 10.1016/j.ab.2008.11.021 |
| الإتاحة: | https://doi.org/10.1016/j.ab.2008.11.021Test https://hal.science/hal-01527917Test https://hal.science/hal-01527917/documentTest https://hal.science/hal-01527917/file/nihms500790.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.E69D2DED |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.ab.2008.11.021 |
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