رسالة جامعية
Differential properties of N- and VE-cadherin in vascular endothelium ; Propriétés différentielles de la VE- et de la N-cadhérine dans l'endothelium vasculaire
| العنوان: | Differential properties of N- and VE-cadherin in vascular endothelium ; Propriétés différentielles de la VE- et de la N-cadhérine dans l'endothelium vasculaire |
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| المؤلفون: | Gentil Dit Maurin, Alice |
| المساهمون: | Physiopathologie vasculaire : interactions cellulaires, signalisation et vieillissement, Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Joseph-Fourier - Grenoble I, Philippe HUBER(phuber@cea.fr) |
| المصدر: | https://theses.hal.science/tel-00507102Test ; Biologie cellulaire. Université Joseph-Fourier - Grenoble I, 2010. Français. ⟨NNT : ⟩. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2010 |
| المجموعة: | HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
| مصطلحات موضوعية: | Endothelial cells, VE-cadherin, N-cadherin, p120 catenin, lipid raft, Cellules endothéliales, VE-cadhérine, N-cadhérine, angiogenèse, p120 caténine, radeaux de cholestérol, PECAM, [SDV.BC]Life Sciences [q-bio]/Cellular Biology |
| الوصف: | Endothelium integrity is maintained by junctional adhesion structures, in particular adherens junctions. Classical cadherins are the main molecule belonging to these structures. Two classical cadherins are expressed in the endothelium. First, the vascular endothelial cadherin or VE-cadherin is only expressed in endothelial cells and located to adherens junctions. Several studies have shown that this molecule is strictly necessary during vascular development. The second classical endothelial cadherin is represented by the neural cadherin or N-cadherin. Contrary to classical cadherins, the endothelial N-cadherin shows diffuse location on the entirely cell surface and interacts with mural cells for vessel stabilization. Moreover, its role in angiogenic process is still debated. The purpose of our study was to decipher the differential role of N- and VE-cadherin in endothelial cells and during angiogenesis. We have shown that VE-cadherin was able to organize and displace N-cadherin location on the cell surface. Moreover, VE-cadherin binding to p120 catenin is required for N-cadherin junctional exclusion. Furthermore, endothelial lipid rafts, known to organize classical cadherin at the membrane, contain exclusively VE-cadherin, where it specifically binds p120 catenin. So, this protein complex could form a platform that stabilizes cadherin at the cell membrane and in the same way, strengthens intercellular interactions. Also, we have found that three endothelial genes : PECAM, Flk-1 and Tie-2, could be transcriptionally controlled by VE-cadherin in a ES cell-derived endothelial differential model. We didn't found this molecular mechanism but, a cross talk between the VE-cadherin dependant PECAM regulation and adherens molecule to extracellular matrix is possible. In the end, we characterized a new role of VE-cadherin. This adherent molecule might be crucial to endothelium biology, especially in the control of important endothelial proteins. ; L'intégrité de l'endothelium vasculaire est maintenue par des structures ... |
| نوع الوثيقة: | doctoral or postdoctoral thesis |
| اللغة: | French |
| العلاقة: | tel-00507102; https://theses.hal.science/tel-00507102Test; https://theses.hal.science/tel-00507102/documentTest; https://theses.hal.science/tel-00507102/file/Manuscrit_format_13_07_2010.pdfTest |
| الإتاحة: | https://theses.hal.science/tel-00507102Test https://theses.hal.science/tel-00507102/documentTest https://theses.hal.science/tel-00507102/file/Manuscrit_format_13_07_2010.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.6480011F |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |