دورية أكاديمية
dnaJ : a New Approach to Identify Species within the Genus Enterobacter
| العنوان: | dnaJ : a New Approach to Identify Species within the Genus Enterobacter |
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| المؤلفون: | Hernandez-Alonso, Enrique, Barreault, Simon, Augusto, Luis, Jatteau, Pierre, Villet, Millie, Tissieres, Pierre, Doucet-Populaire, F., Bourgeois-Nicolaos, Nadege |
| المساهمون: | Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 2165-0497 ; Microbiology Spectrum ; https://hal.science/hal-03610352Test ; Microbiology Spectrum, 2021, 9 (3), ⟨10.1128/Spectrum.01242-21⟩. |
| بيانات النشر: | HAL CCSD American Society for Microbiology |
| سنة النشر: | 2021 |
| المجموعة: | HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; The taxonomy of the genus Enterobacter can be confusing and has been considerably revised in recent years. We propose a PCR and amplicon sequencing technique based on a partial sequence of the dnaJ gene for species assignment consistent with DNA-DNA digital hybridization (dDDH) and pairwise average nucleotide identity (ANI). We performed a validation of the method by comparing the type strains of each species, sequences obtained from the GenBank database, and clinical specimens. Our results show that the polymorphism of the target sequence of dnaJ allows the identification of species. Using this gene, we assigned the species to 100 strains deposited in the GenBank database that were consistent with the species assignment by dDDH and ANI. The analysis showed that using the partial dnaJ sequence is congruent with WGS as far as correct identification of Enterobacter species is concerned. Finally, we applied our dnaJ method on a national collection of 68 strains identified as Enterobacter isolated from the blood cultures of premature babies using an algorithm based on a type-strain library and the SeqScape software. For the first time, we identified Enterobacter quasihormaechei in blood cultures from four neonatal sepsis cases. We also noticed a higher prevalence of E. bugandensis (36.3%; 32/88) and E. xiangfangensis (46.5%; 41/88). E. bugandensis is a novel species recently described specifically in instances of neonatal sepsis. In conclusion, sequencing a part of the dnaJ gene could be a quick, more economical, and highly discriminating method of identifying Enterobacter species in clinical practice and research. ; La taxonomie du genre Enterobacter peut prêter à confusion et a été considérablement révisée ces dernières années. Nous proposons une technique de PCR et de séquençage d'amplicons basée sur une séquence partielle du gène dnaJ pour une assignation des espèces cohérente avec l'hybridation numérique ADN-ADN (dDDH) et l'identité nucléotidique moyenne par paire (ANI). Nous avons ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03610352; https://hal.science/hal-03610352Test; PUBMEDCENTRAL: PMC8694106 |
| DOI: | 10.1128/Spectrum.01242-21 |
| الإتاحة: | https://doi.org/10.1128/Spectrum.01242-21Test https://hal.science/hal-03610352Test |
| رقم الانضمام: | edsbas.A91065B0 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/Spectrum.01242-21 |
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