دورية أكاديمية
In vitro cellular responses to silicon carbide nanoparticles: impact of physico-chemical features on pro-inflammatory and pro-oxidative effects
| العنوان: | In vitro cellular responses to silicon carbide nanoparticles: impact of physico-chemical features on pro-inflammatory and pro-oxidative effects |
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| المؤلفون: | Pourchez, Jérémie, Forest, Valérie, Boumahdi, Najih, Boudard, Delphine, Tomatis, Maura, Fubini, Bice, Herlin-Boime, Nathalie, Leconte, Yann, Guilhot, Bernard, Cottier, Michèle, Grosseau, Philippe |
| المساهمون: | Institut Fédératif de Recherche en Sciences et Ingénierie de la Santé (IFRESIS), Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Ecole Nationale Supérieure des Mines de St Etienne (ENSM ST-ETIENNE)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interdisciplinaire des Nanoparticules Aérosolisées (LINA-ENSME), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT), Centre Ingénierie et Santé (CIS-ENSMSE), Département Poudres et Matériaux Multi-Composants (P2MC-ENSMSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-SPIN, Centre Sciences des Processus Industriels et Naturels (SPIN-ENSMSE), Laboratoire des Procédés en Milieux Granulaires (LPMG-EMSE), Institut Mines-Télécom Paris (IMT)-Institut Mines-Télécom Paris (IMT)-Centre National de la Recherche Scientifique (CNRS), Service d'Histologie Embryologie, Centre Hospitalier Universitaire de Saint-Etienne CHU Saint-Etienne (CHU ST-E), Dipartimento di Chimica, Università degli studi di Torino = University of Turin (UNITO), "G. Scansetti" Interdepartmental Center for Studies on Asbestos and other Toxic Particulates, Laboratoire Francis PERRIN (LFP - URA 2453), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), CHU Saint-Etienne, Université de Turin, CEA Saclay |
| المصدر: | ISSN: 1388-0764. |
| بيانات النشر: | HAL CCSD Springer Verlag |
| سنة النشر: | 2012 |
| المجموعة: | HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
| مصطلحات موضوعية: | Silicon carbide nanoparticles, Laser pyrolysis, Sol-gel, Biological activity, Toxicity, Macrophage cell line, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, [SDV.TOX]Life Sciences [q-bio]/Toxicology |
| الوصف: | International audience ; Silicon carbide is an extremely hard, wear resistant, and thermally stable material with particular photoluminescence and interesting biocompatibility properties. For this reason, it is largely employed for industrial applications such as ceramics. More recently, nano-sized SiC particles were expected to enlarge their use in several fields such as composite supports, power electronics, biomaterials, etc. However, their large-scaled development is restricted by the potential toxicity of nanoparticles related to their manipulation and inhalation. This study aimed at synthesizing (by laser pyrolysis or sol-gel methods), characterizing physico-chemical properties of six samples of SiC nanopowders, then determining their in vitro biological impact(s). Using a macrophage cell line, toxicity was assessed in terms of cell membrane damage (LDH release), inflammatory effect (TNF-α production), and oxidative stress (reactive oxygen species generation). None of the six samples showed cytotoxicity while remarkable pro-oxidative reactions and inflammatory response were recorded, whose intensity appears related to the physico-chemical features of nano-sized SiC particles. In vitro data clearly showed an impact of the extent of nanoparticle surface area and the nature of crystalline phases (α-SiC vs. β-SiC) on the TNF-α production, a role of surface iron on free radical release, and of the oxidation state of the surface on cellular H2O2 production. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-00734434; https://hal.science/hal-00734434Test; https://hal.science/hal-00734434/documentTest; https://hal.science/hal-00734434/file/JP-JNR-14-Orig.pdfTest |
| DOI: | 10.1007/s11051-012-1143-7 |
| الإتاحة: | https://doi.org/10.1007/s11051-012-1143-7Test https://hal.science/hal-00734434Test https://hal.science/hal-00734434/documentTest https://hal.science/hal-00734434/file/JP-JNR-14-Orig.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.C3FFF5AA |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s11051-012-1143-7 |
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