دورية أكاديمية
Using a multiomics approach to unravel a septic shock specific signature in skeletal muscle
| العنوان: | Using a multiomics approach to unravel a septic shock specific signature in skeletal muscle |
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| المؤلفون: | Duceau, Baptiste, Blatzer, Michael, Bardon, Jean, Chaze, Thibault, Giai Gianetto, Quentin, Castelli, Florence, Fenaille, François, Duarte, Lucie, Lescot, Thomas, Tresallet, Christophe, Riou, Bruno, Matondo, Mariette, Langeron, Olivier, Rocheteau, Pierre, Chrétien, Fabrice, Bouglé, Adrien |
| المساهمون: | Neuropathologie expérimentale - Experimental neuropathology, Institut Pasteur Paris (IP)-Université Paris Descartes - Paris 5 (UPD5), CHU Pitié-Salpêtrière AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Henri Mondor, Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology (UTechS MSBio), Institut Pasteur Paris (IP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur Paris (IP)-Centre National de la Recherche Scientifique (CNRS), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), MetaboHUB, MetaboHUB-IDF, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-MetaboHUB-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Small molecule analysis and omics (SMART-OMICS), Service de Pharmacologie et Immunoanalyse (SPI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Institut des Sciences du Vivant Frédéric JOLIOT (JOLIOT), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Médicaments et Technologies pour la Santé (MTS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-MetaboHUB-IDF, Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-MetaboHUB-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-MetaboHUB-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire Innovations en Spectrométrie de Masse pour la santé (LI-MS), CEA- Saclay (CEA), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université Paris-Saclay, CHU Saint-Antoine AP-HP, Sorbonne Université (SU), Centre Hospitalier Sainte Anne Paris, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), GHU Paris Psychiatrie et Neurosciences, The study was funded by a grant of the French Ministry of Higher Education, Research and Innovation (Agence Nationale de la Recherche ANR-17-CE14-0035) to F. Chrétien. Drs A. Bouglé and B. Duceau were the recipients of a research fellowship grant from the Institut Pasteur (Paris, France) and Assistance Publique – Hôpitaux de Paris (Paris, France)., ANR-17-CE14-0035,PUSHUPS,Etude de la dysfonction des cellules souches musculaires au cours du sepsis(2017) |
| المصدر: | ISSN: 2045-2322. |
| بيانات النشر: | HAL CCSD Nature Publishing Group |
| سنة النشر: | 2022 |
| المجموعة: | HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
| مصطلحات موضوعية: | [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; Sepsis is defined as a dysregulated host response to infection leading to organs failure. Among them, sepsis induces skeletal muscle (SM) alterations that contribute to acquired-weakness in critically ill patients. Proteomics and metabolomics could unravel biological mechanisms in sepsis-related organ dysfunction. Our objective was to characterize a distinctive signature of septic shock in human SM by using an integrative multi-omics approach. Muscle biopsies were obtained as part of a multicenter non-interventional prospective study. Study population included patients in septic shock (S group, with intra-abdominal source of sepsis) and two critically ill control populations: cardiogenic shock (C group) and brain dead (BD group). The proteins and metabolites were extracted and analyzed by High-Performance Liquid Chromatography-coupled to tandem Mass Spectrometry, respectively. Fifty patients were included, 19 for the S group (53% male, 64 ± 17 years, SAPS II 45 ± 14), 12 for the C group (75% male, 63 ± 4 years, SAPS II 43 ± 15), 19 for the BD group (63% male, 58 ± 10 years, SAPS II 58 ± 9). Biopsies were performed in median 3 days [interquartile range 1–4]) after intensive care unit admission. Respectively 31 patients and 40 patients were included in the proteomics and metabolomics analyses of 2264 proteins and 259 annotated metabolites. Enrichment analysis revealed that mitochondrial pathways were significantly decreased in the S group at protein level: oxidative phosphorylation (adjusted p = 0.008); branched chained amino acids degradation (adjusted p = 0.005); citrate cycle (adjusted p = 0.005); ketone body metabolism (adjusted p = 0.003) or fatty acid degradation (adjusted p = 0.008). Metabolic reprogramming was also suggested (i) by the differential abundance of the peroxisome proliferator-activated receptors signaling pathway (adjusted p = 0.007), and (ii) by the accumulation of fatty acids like octanedioic acid dimethyl or hydroxydecanoic. Increased polyamines and depletion of ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/36335235; hal-03996889; https://hal.science/hal-03996889Test; https://hal.science/hal-03996889/documentTest; https://hal.science/hal-03996889/file/s41598-022-23544-8.pdfTest; PUBMED: 36335235; PUBMEDCENTRAL: PMC9637214; WOS: 000879722100064 |
| DOI: | 10.1038/s41598-022-23544-8 |
| الإتاحة: | https://doi.org/10.1038/s41598-022-23544-8Test https://hal.science/hal-03996889Test https://hal.science/hal-03996889/documentTest https://hal.science/hal-03996889/file/s41598-022-23544-8.pdfTest |
| حقوق: | http://creativecommons.org/licenses/byTest/ ; info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.1C69DC4B |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/s41598-022-23544-8 |
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