المؤلفون: Mari, Stéphane, Bailly, Christophe, Thomine, Sébastien

المساهمون: Biochimie et Physiologie Moléculaire des Plantes (BPMP), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE20-0019,ISISTOR,Amélioration du contenu en fer de la graine(2016)

المصدر: ISSN: 0264-6021.

مصطلحات موضوعية: iron, biofortification, nutrition, remobilization, seed, vacuole, [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology

الوصف: International audience ; To ensure the success of the new generation in annual species, the mother plant transfers a large proportion of the nutrients it has accumulated during its vegetative life to the next generation through its seeds. Iron (Fe) is required in large amounts to provide the energy and redox power to sustain seedling growth. However, free Fe is highly toxic as it leads to the generation of reactive oxygen species. Fe must, therefore, be tightly bound to chelating molecules to allow seed survival for long periods of time without oxidative damage. Nevertheless, when conditions are favorable, the seed's Fe stores have to be readily remobilized to achieve the transition toward active photosynthesis before the seedling becomes able to take up Fe from the environment. This is likely critical for the vigor of the young plant. Seeds constitute an important dietary source of Fe, which is essential for human health. Understanding the mechanisms of Fe storage in seeds is a key to improve their Fe content and availability in order to fight Fe deficiency. Seed longevity, germination efficiency and seedling vigor are also important traits that may be affected by the chemical form under which Fe is stored. In this review, we summarize the current knowledge on seed Fe loading during development, long-term storage and remobilization upon germination. We highlight how this knowledge may help seed Fe biofortification and discuss how Fe storage may affect the seed quality and germination efficiency.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31950999; hal-02445553; https://hal.science/hal-02445553Test; https://hal.science/hal-02445553/documentTest; https://hal.science/hal-02445553/file/Mari%20et%20al%20Biochemical%20Journal%202020.pdfTest; PRODINRA: 492706; PUBMED: 31950999; WOS: 000512948100016

الإتاحة: https://doi.org/10.1042/BCJ20190188Test
https://hal.science/hal-02445553Test
https://hal.science/hal-02445553/documentTest
https://hal.science/hal-02445553/file/Mari%20et%20al%20Biochemical%20Journal%202020.pdfTest

المؤلفون: Urrutia-Jalabert, Rocío, Lara, Antonio, Barichivich, Jonathan, Vergara, Nicolás, Rodriguez, Carmen Gloria, Piper, Frida I.

المساهمون: Laboratoire des Sciences du Climat et de l'Environnement Gif-sur-Yvette (LSCE), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica, FONDECYT: 1130546, 1171496 NE/SE011811/1, 1190927 15110009 3160258, REDI170117, 7818I20003, We thank the National Forest Service (CONAF) for their permission to work in the Alerce Costero National Park and to Mr. Alfredo N??ez and Mrs. Elba Ojeda for their support during fieldwork and their permanent willingness to protect Alerce forests. We also thank Mar?a Paz Pe?a for her assistance during fieldwork and Prof. Jos? D?rner, PI of the Fondecyt Project 1130546, for facilitating soil water content data from FN for the summer 2015-2016. Funding. RU-J acknowledges, the Fondecyt Grant 1171496, the PAI-CONICYT Inserci?n en el Sector Productivo 7818I20003 Grant, the Fondecyt Postdoctoral Grant 3160258, Proyecto REDES ETAPA INICIAL, Convocatoria 2017 REDI170117, the CONICYT/FONDAP 15110009, and the ARBOLES CONICYT-NERC-UK Project NE/SE011811/1. FP acknowledges to FONDECYT Grant 1190927.

المصدر: ISSN: 1664-462X ; Frontiers in Plant Science ; https://hal.science/hal-02927386Test ; Frontiers in Plant Science, 2020, 11, ⟨10.3389/fpls.2020.00905⟩.

مصطلحات موضوعية: carbon limitation, drought, Fitzroya cupressoides, non-structural carbohydrates, remobilization, starch, sugars, tree-growth, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology

الوصف: International audience ; There is an ongoing debate on whether a drought induced carbohydrate limitation (source limitation) or a direct effect of water shortage (sink limitation) limit growth under drought. In this study, we investigated the effects of the two driest summers recorded in southern Chile in the last seven decades, on the growth and non-structural carbohydrates (NSC) concentrations of the slow-growing conifer Fitzroya cupressoides. Specifically, we studied the seasonal variation of NSC in saplings and adults one and two years after the occurrence of a 2 year-summer drought at two sites of contrasting precipitation and productivity (mesic-productive vs. rainy-less productive). We also evaluated radial growth before, during and after the drought, and predicted that drought could have reduced growth. If drought caused C source limitation, we expected that NSCs will be lower during the first than the second year after drought. Conversely, similar NSC concentrations between years or higher NSC concentrations in the first year would be supportive of sink limitation. Also, due to the lower biomass of saplings compared with adults, we expected that saplings should experience stronger seasonal NSC remobilization than adults. We confirmed this last expectation. Moreover, we found no significant growth reduction during drought in the rainy site and a slightly significant growth reduction at the mesic site for both saplings and adults. Across organs and in both sites and age classes, NSC, starch, and sugar concentrations were generally higher in the first than in the second year following drought, while NSC seasonal remobilization was generally lower. Higher NSC concentrations along with lower seasonal NSC remobilization during the first post-drought year are supportive of sink limitation. However, as these results were found at both sites while growth decreased slightly and just at the mesic site, limited growth only is unlikely to have caused NSC accumulation. Rather, these results suggest that the ...

العلاقة: hal-02927386; https://hal.science/hal-02927386Test; https://hal.science/hal-02927386/documentTest; https://hal.science/hal-02927386/file/fpls-11-00905.pdfTest

الإتاحة: https://doi.org/10.3389/fpls.2020.00905Test
https://hal.science/hal-02927386Test
https://hal.science/hal-02927386/documentTest
https://hal.science/hal-02927386/file/fpls-11-00905.pdfTest

المؤلفون: Warakanont, Jaruswan, Li-Beisson, Yonghua, Benning, Christoph

المساهمون: Environnement, Bioénergie, Microalgues et Plantes (EBMP), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Kasetsart University Bangkok, Thailand (KU), Michigan State University East Lansing, Michigan State University System

المصدر: ISSN: 0032-0781.

مصطلحات موضوعية: $Chlamydomonas\ reinhardtii$, lipid catabolism, SUGAR DEPENDENT1, triacylglycerol lipase, N recovery, TAG remobilization, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Degradation of the storage compound triacylglycerol (TAG) is a crucial process in response to environmental stimuli. Failing to respond properly may be detrimental for survival. In microalgae, this process is important for re-growth when conditions become favorable after cells have experienced stresses. Mobilization of TAG is initiated by actions of lipases causing the release of glycerol and free fatty acids, which can be further broken down for energy production or recycled to synthesize membrane lipids. Although key enzymes in the process, TAG lipases remain to be characterized in the model green alga $Chlamydomonas\ reinhardtii$. Here we describe the functional analysis of a putative TAG lipase, i.e. CrLIP4, which shares 44% amino acid identity with the major TAG lipase in $Arabidopsis$ (SUGAR DEPENDENT1- SDP1). The $CrLIP4$ transcript level was down regulated during nitrogen deprivation (ND) when TAG accumulates, but was upregulated during nitrogen recovery (NR) when TAG wasdegraded. Both artificial microRNA knockdown and insertional knockout mutants showed a delay in TAG mobilization during NR. The difference in TAG degradation was more pronounced when the cultures were incubated without acetate in the dark. Furthermore, the $crlip4$ knock-out mutant over-accumulated TAG during optimal growth conditions. Taken together, the results suggest to us that CrLIP4 likely acts as a TAG lipase and plays a major role in TAG homeostasis in $Chlamydomonas$.

العلاقة: cea-02096094; https://cea.hal.science/cea-02096094Test; https://cea.hal.science/cea-02096094/documentTest; https://cea.hal.science/cea-02096094/file/Warakamont-2019-CrLIP4-ms5.pdfTest

الإتاحة: https://doi.org/10.1093/pcp/pcz037Test
https://cea.hal.science/cea-02096094Test
https://cea.hal.science/cea-02096094/documentTest
https://cea.hal.science/cea-02096094/file/Warakamont-2019-CrLIP4-ms5.pdfTest

المؤلفون: Chen, Qinwu, Shinozaki, Daiki, Luo, Jie, Pottier, Mathieu, Havé, Marien, Marmagne, Anne, Reisdorf-Cren, Michèle, Chardon, Fabien, Thomine, Sébastien, Yoshimoto, Kohki, Masclaux-Daubresse, Céline

المساهمون: Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Meiji University Tokyo, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Approches intégratives du Transport Ionique (MINION), Département Biologie Cellulaire (BioCell), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), ANR-10-LABX-0040,SPS,Saclay Plant Sciences(2010), ANR-12-ADAP-0010,AUTOADAPT,Role de l'AUTOphagie dans l'ADAPTation des plantes aux limitations en nitrate ou suphate dans leur environnement(2012)

المصدر: ISSN: 2073-4409 ; Cells ; https://hal.science/hal-02367568Test ; Cells, 2019, 8 (11), ⟨10.3390/cells8111426⟩.

مصطلحات موضوعية: iron, leaf senescence, nitrogen remobilization, nitrogen use efficiency, plant proteases, zinc, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Nutrient recycling and mobilization from organ to organ all along the plant lifespan is essential for plant survival under changing environments. Nutrient remobilization to the seeds is also essential for good seed production. In this review, we summarize the recent advances made to understand how plants manage nutrient remobilization from senescing organs to sink tissues and what is the contribution of autophagy in this process. Plant engineering manipulating autophagy for better yield and plant tolerance to stresses will be presented.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/31726766; hal-02367568; https://hal.science/hal-02367568Test; https://hal.science/hal-02367568/documentTest; https://hal.science/hal-02367568/file/Chen%20et%20al.%20Cells%202020.pdfTest; PUBMED: 31726766; WOS: 000502266700124

الإتاحة: https://doi.org/10.3390/cells8111426Test
https://hal.science/hal-02367568Test
https://hal.science/hal-02367568/documentTest
https://hal.science/hal-02367568/file/Chen%20et%20al.%20Cells%202020.pdfTest

المؤلفون: Pottier, Mathieu, Dumont, Jean, Masclaux-Daubresse, Céline, Thomine, Sébastien

المساهمون: Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Jean-Pierre Bourgin (IJPB), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, Approches intégratives du Transport Ionique (MINION), Département Biologie Cellulaire (BioCell), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Département Biochimie, Biophysique et Biologie Structurale (B3S), ANR-16-CE20-0019,ISISTOR,Amélioration du contenu en fer de la graine(2016)

المصدر: ISSN: 0022-0957.

مصطلحات موضوعية: iron loading, iron recycling, leaf senescence, metal, remobilization, 57Fe, premature senescence, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics

الوصف: International audience ; Micronutrient deficiencies affect a large part of the world’s population. These deficiencies are mostly due to the consumption of grains with insufficient content of iron (Fe) or zinc (Zn). Both de novo uptake by roots and recycling from leaves may provide seeds with nutrients. Autophagy, which is a conserved mechanism for nutrient recycling in eukaryotes, was shown to be involved in nitrogen remobilization to seeds. Here, we have investigated the role of this mechanism in micronutrient translocation to seeds. We found that Arabidopsis thaliana plants impaired in autophagy display defects in nutrient remobilization to seeds. In the atg5-1 mutant, which is completely defective in autophagy, the efficiency of Fe translocation from vegetative organs to seeds was severely decreased even when Fe was provided during seed formation. Combining atg5-1 with the sid2 mutation that counteracts premature senescence associated with autophagy deficiency and using 57Fe pulse labeling, we propose a two-step mechanism in which Fe taken up de novo during seed formation is first accumulated in vegetative organs and subsequently remobilized to seeds. Finally, we show that translocation of Zn and manganese (Mn) to seeds is also dependent on autophagy. Fine-tuning autophagy during seed formation opens up new possibilities to improve micronutrient remobilization to seeds

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/30395253; hal-02173634; https://hal.science/hal-02173634Test; https://hal.science/hal-02173634/documentTest; https://hal.science/hal-02173634/file/ery388.pdfTest; PRODINRA: 468557; PUBMED: 30395253; PUBMEDCENTRAL: PMC6363094; WOS: 000459350700013

الإتاحة: https://doi.org/10.1093/jxb/ery388Test
https://hal.science/hal-02173634Test
https://hal.science/hal-02173634/documentTest
https://hal.science/hal-02173634/file/ery388.pdfTest

المؤلفون: Resongles, Eléonore, Casiot, Corinne, Freydier, Remi, Le Gall, Marion, Elbaz-Poulichet, Françoise

المساهمون: Hydrosciences Montpellier (HSM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences du Climat et de l'Environnement Gif-sur-Yvette (LSCE), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Géochimie Des Impacts (GEDI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA))

المصدر: ISSN: 0375-6742 ; Journal of Geochemical Exploration ; https://hal.science/hal-02063551Test ; Journal of Geochemical Exploration, 2015, 158, pp.132--142. ⟨10.1016/j.gexplo.2015.07.010⟩.

مصطلحات موضوعية: Metal and metalloid load, High-temporal resolution sampling, Flood event, Contaminated particles remobilization, Mining pollution, Mediterranean watershed, [SDU.STU.HY]Sciences of the Universe [physics]/Earth Sciences/Hydrology, [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry

الوصف: International audience ; The variation of dissolved and particulate metal(loid) concentrations was investigated during a Mediterranean flood in a former mining watershed, the Gardon River (SE France), using high-temporal resolution sampling. Dissolved antimony originating from the Upper Gardon River watershed underwent dilution during flood. Conversely, dissolved As and particulate As, Pb, Zn, Cd and Tl concentrations exhibited increased values during rising flood compared to low and receding flow conditions, with a double-peak shape. As, Pb, Cd and Tl concentration data in suspended particulate matter from this double-peak were distributed along two different correlation lines, showing the successive mobilization of two groups of particles highly enriched with As and Cd or Pb and Tl, both inherited from ancient Pb/Zn mines. Metal(loid) loads during the monitored 24h-flood event were: 0.5kg for Cd, 19.4kg for Sb and 204kg for As in the dissolved phase and 24kg for Cd, 38kg for Tl, 94kg for Sb, 1915kg for As, 2860kg for Pb and 5214kg for Zn in the particulate phase. Altogether, these results highlighted the importance of floods in the mobilization of metals and metalloids from ancient mining sites in Mediterranean regions, showing the need for high-temporal resolution monitoring of flood events to accurately assess the long-term contribution of mining activity to metal(loid) loads of downstream watershed.

العلاقة: hal-02063551; https://hal.science/hal-02063551Test; https://hal.science/hal-02063551/documentTest; https://hal.science/hal-02063551/file/Crue%202015%20version%20r%C3%A9vis%C3%A9e.pdfTest

الإتاحة: https://doi.org/10.1016/j.gexplo.2015.07.010Test
https://hal.science/hal-02063551Test
https://hal.science/hal-02063551/documentTest
https://hal.science/hal-02063551/file/Crue%202015%20version%20r%C3%A9vis%C3%A9e.pdfTest

المؤلفون: Cagnon, Caroline, Mirabella, Boris, Nguyen, Hoa Mai, Beyly-Adriano, Audrey, Bouvet, Séverine, Cuiné, Stéphan, Beisson, Fred, Peltier, G., Li-Beisson, Yonghua

المساهمون: Environnement, Bioénergie, Microalgues et Plantes (EBMP), Institut de Biosciences et Biotechnologies d'Aix-Marseille (ex-IBEB) (BIAM), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ANR-12-BIME-0001,DIESALG,Production de biodiesel par microalgues(2012)

المصدر: ISSN: 1754-6834 ; Biotechnology for Biofuels ; https://hal.science/hal-03378490Test ; Biotechnology for Biofuels, 2013, 6 (1), ⟨10.1186/1754-6834-6-178⟩.

مصطلحات موضوعية: Chlamydomonas mutants, Flow cytometry, Genetic screen, Lipid remobilization, Microalgal oil, Nile red, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Oils produced by microalgae are precursors to biodiesel. To achieve a profitable production of biodiesel from microalgae, identification of factors governing oil synthesis and turnover is desirable. The green microalga Chlamydomonas reinhardtii is amenable to genetic analyses and has recently emerged as a model to study oil metabolism. However, a detailed method to isolate various types of oil mutants that is adapted to Chlamydomonas has not been reported.ResultsWe describe here a forward genetic approach to isolate mutants altered in oil synthesis and turnover from C. reinhardtii. It consists of a three-step screening procedure: a primary screen by flow cytometry of Nile red stained transformants grown in 96-deep-well plates under three sequential conditions (presence of nitrogen, then absence of nitrogen, followed by oil remobilization); a confirmation step using Nile red stained biological triplicates; and a validation step consisting of the quantification by thin layer chromatography of oil content of selected strains. Thirty-one mutants were isolated by screening 1,800 transformants generated by random insertional mutagenesis (1.7%). Five showed increased oil accumulation under the nitrogen-replete condition and 13 had altered oil content under nitrogen-depletion. All mutants were affected in oil remobilization.ConclusionThis study demonstrates that various types of oil mutants can be isolated in Chlamydomonas based on the method set-up here, including mutants accumulating oil under optimal biomass growth. The strategy conceived and the protocol set-up should be applicable to other microalgal species such as Nannochloropsis and Chlorella, thus serving as a useful tool in Chlamydomonas oil research and algal biotechnology.

العلاقة: hal-03378490; https://hal.science/hal-03378490Test; https://hal.science/hal-03378490/documentTest; https://hal.science/hal-03378490/file/Cagnon2013.pdfTest

الإتاحة: https://doi.org/10.1186/1754-6834-6-178Test
https://hal.science/hal-03378490Test
https://hal.science/hal-03378490/documentTest
https://hal.science/hal-03378490/file/Cagnon2013.pdfTest

المؤلفون: Herranz, L.E., Ball, J., Auvinen, A., Bottomley, D., Dehbi, A., Housiadas, C., Piluso, P., Layly, V., Parozzi, F., Reeks, M.

المساهمون: Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas (CIEMAT), Atomic Energy of Canada Limited (AECL), VTT Technical Research Centre of Finland (VTT), JRC Institute for Transuranium Elements Karlsruhe (ITU ), European Commission - Joint Research Centre Karlsruhe (JRC), Paul Scherrer Institute (PSI), National Center for Scientific Research "Demokritos" (NCSR), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de Radioprotection et de Sûreté Nucléaire (IRSN), CESI RICERCA, Newcastle University Newcastle

المصدر: Progress in Nuclear Energy ; https://hal.science/hal-02972636Test ; Progress in Nuclear Energy, 2010, 52 (1), pp.120-127. ⟨10.1016/j.pnucene.2009.09.013⟩

مصطلحات موضوعية: Aerosol transport, CFD models, Code validation, Complex geometries, Complex structure, Concrete cracks, Concrete wall, Current capability, Data reviews, Dry and wet, Empirical data, Experimental data, Experimental program, Further development, Integral experiments, International projects, Micro-scales, Particle depositions, Primary circuits, Random Walk, Re-suspension, Remobilization, Risk scenarios, Severe accident, Source term, Source terms, Stochastic treatment, Turbulence interactions, Accidents, Atmospheric aerosols

الوصف: International audience ; The 6th FWP SARNET project launched a set of studies to enhance understanding and predictability of relevant-risk scenarios where uncertainties related to aerosol phenomena were still significant: retention in complex structures, such as steam generator by-pass SGTR sequences or cracks in concrete walls of an over-pressurised containment, and primary circuit deposit remobilization, either as vapours (revaporisation) or aerosols (resuspension). This paper summarizes the major advances achieved. Progress has been made on aerosol scrubbing in complex structures. Models based on empirical data (ARISG) and improvements to previous codes (SPARC) have been proposed, respectively, for dry and wet aerosol retention, but, further development and validation remains, as was noted during the ARTIST international project and potential successors. New CFD models for particle-turbulence interactions have been developed based on random walk stochastic treatments and have shown promise in accurately describing particle deposition rates in complex geometries. Aerosol transport in containment concrete cracks is fairly well understood, with several models developed but validation was limited. Extension of such validation against prototypic data will be feasible through an ongoing joint experimental program in the CEA COLIMA facility under the 6th Framework PLINIUS platform. Primary deposit revaporisation has been experimentally demonstrated on samples from the Phebus-FP project. Data review has pinpointed variables affecting the process, particularly temperature. Available models have been satisfactorily used to interpret separate-effect tests, but performing integral experiments, where revaporisation is likely combined with other processes, still pose a difficult challenge. Further experimental data as well as modelling efforts seem to be necessary to get a full understanding. Resuspension, sometimes referred to as mechanical remobilization, has been recently addressed in SARNET and although a set of models ...

العلاقة: hal-02972636; https://hal.science/hal-02972636Test

الإتاحة: https://doi.org/10.1016/j.pnucene.2009.09.013Test
https://hal.science/hal-02972636Test