دورية أكاديمية
Simulations of amperometric monitoring of exocytosis: moderate pH variations within the cell-electrode cleft with the buffer diffusion
| العنوان: | Simulations of amperometric monitoring of exocytosis: moderate pH variations within the cell-electrode cleft with the buffer diffusion |
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| المؤلفون: | Bouret, Yann, Guille-Collignon, Manon, Lemaître, Frédéric |
| المساهمون: | Institut de Physique de Nice (INPHYNI), Université Nice Sophia Antipolis (1965 - 2019) (UNS)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UniCA), Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640) (PASTEUR), Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-École normale supérieure - Paris (ENS-PSL), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
| المصدر: | ISSN: 1618-2642. |
| بيانات النشر: | HAL CCSD Springer Verlag |
| سنة النشر: | 2021 |
| المجموعة: | HAL Université Côte d'Azur |
| مصطلحات موضوعية: | vesicular exocytosis, amperometry, ultramicroelectrodes, pH, simulations, [PHYS]Physics [physics] |
| الوصف: | International audience ; Amperometry with ultramicroelectrodes is nowadays a routine technique to investigate neurotransmitter secretion by vesicular exocytosis at the single-cell level. This electroanalytical tool allows one to understand many aspects of the vesicular release in terms of mechanisms. However, the electrochemical detection relies on the oxidation of released neurotransmitters that produce 2H+ and thus the possible acidification of the cell-electrode cleft. In a previous work, we considered a model involving the H+ diffusion or/and its reaction with buffer species. In this article, we report a more general model which takes into account the ability of buffer species to move and to be regenerated within the cell-electrode cleft. As a consequence, the pH within the cleft is still equal to its physiological value regardless of the electrochemical detection of the vesicular release for usual exocytotic cell frequencies. This confirms that amperometry at the single-cell level is a very robust technique for investigating vesicular exocytosis. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-03260889; https://hal.sorbonne-universite.fr/hal-03260889Test; https://hal.sorbonne-universite.fr/hal-03260889/documentTest; https://hal.sorbonne-universite.fr/hal-03260889/file/draft_Proof_hi%20-%20Copie.pdfTest |
| DOI: | 10.1007/s00216-021-03443-z |
| الإتاحة: | https://doi.org/10.1007/s00216-021-03443-zTest https://hal.sorbonne-universite.fr/hal-03260889Test https://hal.sorbonne-universite.fr/hal-03260889/documentTest https://hal.sorbonne-universite.fr/hal-03260889/file/draft_Proof_hi%20-%20Copie.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.5FB8D3 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00216-021-03443-z |
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