دورية أكاديمية
Excitation of histaminergic tuberomamillary neurons by thyrotropin-releasing hormone.
| العنوان: | Excitation of histaminergic tuberomamillary neurons by thyrotropin-releasing hormone. |
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| المؤلفون: | Parmentier, Régis, Kolbaev, Sergej, Klyuch, Boris, P., Vandael, David, Lin, Jian-Sheng, Selbach, Oliver, Haas, Helmut, L., Sergeeva, Olga, A. |
| المساهمون: | Physiologie integrée du système d'éveil, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of neurophysiology, Heinrich Heine Universität Düsseldorf = Heinrich Heine University Düsseldorf, Supported by the DFG SE 1767, SFB 575/3 and 8, INSERM U628 and INSERM grant of JSL to RP |
| المصدر: | ISSN: 0270-6474. |
| بيانات النشر: | HAL CCSD Society for Neuroscience |
| سنة النشر: | 2009 |
| المجموعة: | HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: | posterior hypothalamus, thyrotropin releasing hormone, montirelin, H3-receptors, intracellular recording, sleep EEG, MESH: Action Potentials, MESH: Animals, MESH: Neurons, MESH: Rats, Wistar, MESH: Receptors, Thyrotropin-Releasing Hormone, MESH: Sleep Stages, MESH: Sodium Channels, MESH: Thyrotropin-Releasing Hormone, MESH: Calcium Channels, MESH: Cations, Divalent, MESH: Histamine, MESH: Hypothalamic Area, Lateral, MESH: Inhibitory Postsynaptic Potentials, MESH: Male, MESH: Mice, Knockout, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] |
| الوصف: | International audience ; The histaminergic tuberomamillary nucleus (TMN) controls arousal and attention, and the firing of TMN neurons is state-dependent, active during waking, silent during sleep. Thyrotropin-releasing hormone (TRH) promotes arousal and combats sleepiness associated with narcolepsy. Single-cell reverse-transcription-PCR demonstrated variable expression of the two known TRH receptors in the majority of TMN neurons. TRH increased the firing rate of most (ca 70%) TMN neurons. This excitation was abolished by the TRH receptor antagonist chlordiazepoxide (CDZ; 50 mum). In the presence of tetrodotoxin (TTX), TRH depolarized TMN neurons without obvious change of their input resistance. This effect reversed at the potential typical for nonselective cation channels. The potassium channel blockers barium and cesium did not influence the TRH-induced depolarization. TRH effects were antagonized by inhibitors of the Na(+)/Ca(2+) exchanger, KB-R7943 and benzamil. The frequency of GABAergic spontaneous IPSCs was either increased (TTX-insensitive) or decreased [TTX-sensitive spontaneous IPSCs (sIPSCs)] by TRH, indicating a heterogeneous modulation of GABAergic inputs by TRH. Facilitation but not depression of sIPSC frequency by TRH was missing in the presence of the kappa-opioid receptor antagonist nor-binaltorphimine. Montirelin (TRH analog, 1 mg/kg, i.p.) induced waking in wild-type mice but not in histidine decarboxylase knock-out mice lacking histamine. Inhibition of histamine synthesis by (S)-alpha-fluoromethylhistidine blocked the arousal effect of montirelin in wild-type mice. We conclude that direct receptor-mediated excitation of rodent TMN neurons by TRH demands activation of nonselective cation channels as well as electrogenic Na(+)/Ca(2+) exchange. Our findings indicate a key role of the brain histamine system in TRH-induced arousal. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/19357273; inserm-00387960; https://inserm.hal.science/inserm-00387960Test; https://inserm.hal.science/inserm-00387960/documentTest; https://inserm.hal.science/inserm-00387960/file/Parmentieretal2311_revertedHLH.pdfTest; https://inserm.hal.science/inserm-00387960/file/inserm-00387960_edited.pdfTest; PUBMED: 19357273 |
| DOI: | 10.1523/JNEUROSCI.2976-08.2009 |
| الإتاحة: | https://doi.org/10.1523/JNEUROSCI.2976-08.2009Test https://inserm.hal.science/inserm-00387960Test https://inserm.hal.science/inserm-00387960/documentTest https://inserm.hal.science/inserm-00387960/file/Parmentieretal2311_revertedHLH.pdfTest https://inserm.hal.science/inserm-00387960/file/inserm-00387960_edited.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.51C75775 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1523/JNEUROSCI.2976-08.2009 |
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