المؤلفون: Ciardi, Diego, Rigatelli, Benedetta, Richaud, Emmanuel, Cloitre, Michel, Tournilhac, François

المساهمون: Chimie Moléculaire, Macromoléculaire et Matériaux (UMR7167) (C3M), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Ingénierie des Matériaux Polymères (IMP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Procédés et Ingénierie en Mécanique et Matériaux (PIMM), Conservatoire National des Arts et Métiers CNAM (CNAM), HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM)-Centre National de la Recherche Scientifique (CNRS)-Arts et Métiers Sciences et Technologies, HESAM Université - Communauté d'universités et d'établissements Hautes écoles Sorbonne Arts et métiers université (HESAM), European Project: 860911,VITRIMAT

المصدر: ISSN: 0032-3861 ; Polymer ; https://hal.science/hal-04560377Test ; Polymer, 2024, 297, pp.126864. ⟨10.1016/j.polymer.2024.126864⟩.

مصطلحات موضوعية: semi-crystalline, vitrimers, disulfides exchange, shape-memory, [CHIM.POLY]Chemical Sciences/Polymers

الوصف: International audience ; A statistical copolymer p(E-co-GMA) of ethylene and glycidyle methacrylate (4.5 wt%) is considered as a starting point towards semicrystalline networks with exchangeable links. Application of the binomial law to size exclusion chromatographic data suggests that chains deprived of reactive comonomers would represent less than 6 wt% of the total, allowing to expect the formation of high gel-content networks. Experimentally, up to 98 wt% gel is obtained upon modification with 3,3'-dithiopropionic acid (DTPA). The crosslinking reaction can be performed in the molten state through a 2-steps procedure: 1°) x mol. of a commercial grade p(E-co-GMA), y mol. of DTPA and z mol. of triazabicyclodecene (TBD) are sheared in a twin-screw compounder at 115°C, a temperature right above the melting point of the polymer precursor, without triggerring the epoxy-acid addition; 2°) the thermolatent reactive blend thereby obtained is then crosslinked at 170°C. Samples with x = 1, y = 1 to 4 and z = 0 to 0.8 were thus prepared and evaluated. The presence of TBD accelerates disulfide exchange reaction, possibly by involvement of the thiolate anion whereas it decelerates the epoxy-acid addition. The final networks present several characteristic properties of vitrimers such as insolubility in xylene above the melting point, thermo-activated stress relaxation and creep. Particular shape memory regimes, related to the semicrystalline vitrimer character and the high insoluble fraction are demonstrated. Eventually, production scale-up using continuous reactive extrusion is evaluated.

العلاقة: info:eu-repo/grantAgreement//860911/EU/Marie Skłodowska-Curie Grant agreement /VITRIMAT; hal-04560377; https://hal.science/hal-04560377Test; https://hal.science/hal-04560377/documentTest; https://hal.science/hal-04560377/file/20240219-Manuscript.pdfTest

الإتاحة: https://doi.org/10.1016/j.polymer.2024.126864Test
https://hal.science/hal-04560377Test
https://hal.science/hal-04560377/documentTest
https://hal.science/hal-04560377/file/20240219-Manuscript.pdfTest

المؤلفون: Zhang, Jian, Han, Dong, Wang, Yanhui, Wang, Longwei, Chen, Xuanyu, Qiao, Xiran, Yu, Xin

المساهمون: Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), INL - Matériaux Fonctionnels et Nanostructures (INL - MFN), Institut des Nanotechnologies de Lyon (INL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0026-3672.

مصطلحات موضوعية: chitosan, disulfide, dyes, reagents, indicators, markers and buffers, graphite, horseradish peroxidase, hydrogen peroxide, immobilized enzyme, methylene blue, molybdenum, molybdenum disulfide, nanomaterial, catalysis, chemistry, electrochemical analysis, enzymology, genetic procedures, horseradish, oxidation reduction reaction, procedures, reproducibility, Armoracia, Biosensing Techniques, Disulfides, Electrochemical Techniques, Enzymes, Immobilized, Indicators and Reagents

الوصف: International audience ; A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure. The MoS2 nanosheets are synthesized in situ on a graphite microfiber. The chemical composition and surface morphology of the microfiber hybrid structure has been characterized. The microfiber is shown to display peroxidase-mimicking activity. In the next step, horseradish peroxidase, methylene blue, and chitosan are co-immobilized on the microfiber electrode. The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface. The modified microfiber electrode, best operated at a voltage of − 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3). The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP). [Figure not available: see fulltext.] © 2020, Springer-Verlag GmbH Austria, part of Springer Nature.

العلاقة: hal-03770915; https://hal.science/hal-03770915Test

الإتاحة: https://doi.org/10.1007/s00604-020-04299-3Test
https://hal.science/hal-03770915Test

المؤلفون: Ram, Narendra, Weiss, Norbert, Texier-Nogues, Isabelle, Aroui, Sonia, Andreotti, Nicolas, Pirollet, Fabienne, Ronjat, Michel, Sabatier, Jean-Marc, Darbon, Hervé, Jacquemond, Vincent, de Waard, Michel

المساهمون: Grenoble Institut des Neurosciences (GIN), Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiologie intégrative, cellulaire et moléculaire (PICM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives - Laboratoire d'Electronique et de Technologie de l'Information (CEA-LETI), Direction de Recherche Technologique (CEA) (DRT (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), ERT 62 Ambrilia Biopharma S.A., Université de la Méditerranée - Aix-Marseille 2, Architecture et fonction des macromolécules biologiques (AFMB), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Inserm, Fonds de valorisation du Commissariat à l'Energie Atomique, Région Rhône-Alpes bourse Emergence pour doctorant

المصدر: ISSN: 0021-9258.

مصطلحات موضوعية: MESH: Animals, MESH: CHO Cells, MESH: Scorpions, MESH: Cricetinae, MESH: Cricetulus, MESH: Disulfides, MESH: Drug Carriers, MESH: Humans, MESH: Mice, MESH: Muscle, Skeletal, MESH: Scorpion Venoms, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.BIO]Life Sciences [q-bio]/Biotechnology

الوصف: International audience ; Maurocalcine is a 33-mer peptide initially isolated from the venom of a Tunisian scorpion. It has proved itself valuable as a pharmacological activator of the ryanodine receptor and has helped the understanding of the molecular basis underlying excitation-contraction coupling in skeletal muscles. Because of its positively charged nature, it is also an innovative vector for the cell penetration of various compounds. We report a novel maurocalcine analog with improved properties: (i) the complete loss of pharmacological activity, (ii) preservation of the potent ability to carry cargo molecules into cells, and (iii) coupling chemistries not affected by the presence of internal cysteine residues of maurocalcine. We did this by replacing the six internal cysteine residues of maurocalcine by isosteric 2-aminobutyric acid residues and by adding an additional N-terminal biotinylated lysine (for a proof of concept analog) or an N-terminal cysteine residue (for a chemically competent coupling analogue). Additional replacement of a glutamate residue by alanyl at position 12 further improves the potency of these analogues. Coupling to several cargo molecules or nanoparticles are presented to illustrate the cell penetration potency and usefulness of these pharmacologically inactive analogs.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/18621738; inserm-00355685; https://inserm.hal.science/inserm-00355685Test; https://inserm.hal.science/inserm-00355685/documentTest; https://inserm.hal.science/inserm-00355685/file/MM-Abu-revised-ver2-fig.pdfTest; https://inserm.hal.science/inserm-00355685/file/inserm-00355685_edited.pdfTest; PUBMED: 18621738

الإتاحة: https://doi.org/10.1074/jbc.M804727200Test
https://inserm.hal.science/inserm-00355685Test
https://inserm.hal.science/inserm-00355685/documentTest
https://inserm.hal.science/inserm-00355685/file/MM-Abu-revised-ver2-fig.pdfTest
https://inserm.hal.science/inserm-00355685/file/inserm-00355685_edited.pdfTest

المؤلفون: Veselska, O., Cai, L., Podbevsek, D., Ledoux, G., Guillou, N., Pilet, G., Fateeva, A., Demessence, A.

المساهمون: IRCELYON-C'Durable (CDURABLE), Institut de recherches sur la catalyse et l'environnement de Lyon (IRCELYON), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Multimatériaux et Interfaces (LMI), Institut Lumière Matière Villeurbanne (ILM), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut Lavoisier de Versailles (ILV), Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0020-1669.

مصطلحات موضوعية: Physical and chemical processes, Chemical structure, Ligands, Coordination polymers, Disulfides, [CHIM.CATA]Chemical Sciences/Catalysis, [SDE.ES]Environmental Sciences/Environment and Society

الوصف: SSCI-VIDE+CDFA+OVE:ADM ; International audience ; Two copper(II)-carboxylate disulfide coordination polymers [Cu 2 ((O 2 CPhS) 2 ) 2 (H 2 O) 2 ] n (1, 2) and one copper(I)-thiolate coordination polymer [Cu(p-SPhCO 2 H)] n (3) have been synthesized using either the 4-mercaptobenzoic acid (HSPhCO 2 H) or the 4,4′-dithiodibenzoic acid ((SPhCO 2 H) 2 ) as ligand. These three compounds were characterized by X-ray diffraction, IR, and thermogravimetric analyses. Compounds 1 and 2 are polymorphs with the presence, for both, of dinuclear paddle-wheel copper(II)-carboxylates. In 1, the adjacent dimeric Cu 2 units are linked by two (O 2 CPhS) 2 ligands generating a cyclic loop chain, and in 2, each pair of Cu (II) atoms is linked by four ligands to create 2D networks, that are 2-fold interpenetrated. Compound 3 presents a lamellar structure, with an exceptional thermal and chemical stability, and exhibits intrinsic multiple emission between 485 and 660 nm. The different intensities of these bands generate a cyclic luminescence thermochromism from yellow to green to yellow.

العلاقة: hal-01764052; https://hal.science/hal-01764052Test

الإتاحة: https://doi.org/10.1021/acs.inorgchem.7b03090Test
https://hal.science/hal-01764052Test

المؤلفون: Wahid, Ahmed, Helle, François, Descamps, Véronique, Duverlie, Gilles, Penin, François, Dubuisson, Jean

المساهمون: Institut de biologie de Lille - IBL (IBLI), Université de Lille, Sciences et Technologies-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Université de Lille, Droit et Santé-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Virologie CHU Amiens, CHU Amiens-Picardie, Unité de Virologie clinique et fondamentale (UVCF), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Institut de biologie et chimie des protéines Lyon (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire CHU Lille (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0022-538X.

مصطلحات موضوعية: MESH: Amino Acid Substitution, MESH: Cell Line, MESH: Virus Assembly, MESH: Virus Internalization, MESH: Cysteine, MESH: Disulfides, MESH: Hepacivirus, MESH: Humans, MESH: Mutagenesis, Site-Directed, MESH: Mutant Proteins, MESH: Reverse Genetics, MESH: Viral Envelope Proteins, [SDV]Life Sciences [q-bio]

الوصف: International audience ; Class II membrane fusion proteins have been described in viruses in which the envelope proteins are derived from a precursor polyprotein containing two transmembrane glycoproteins arranged in tandem. Although the second protein, which carries the membrane fusion function, is in general well characterized, the companion protein, which is a protein chaperone for the folding of the fusion protein, is less well characterized for some viruses, like hepatitis C virus (HCV). To investigate the role of the class II companion glycoprotein E1 of HCV, we chose to target conserved cysteine residues in the protein, and we systematically mutated them in a full-length infectious HCV clone by reverse genetics. All the mutants were infectious, albeit with lower titers than the wild-type virus. The reduced infectivity was in part due to a decrease in viral assembly, as revealed by measurement of intracellular infectivity and by quantification of core protein released from cells transfected with mutant genomes. Analyses of mutated proteins did not show any major defect in folding. However, the mutations reduced virus stability, and they could also affect the density of infectious viral particles. Mutant viruses also showed a defect in cell-to-cell transmission. Finally, our data indicate that HCV glycoprotein E1 can also affect the fusion protein E2 by modulating its recognition by the cellular coreceptor CD81. Therefore, in the context of HCV, our data identify an additional function of a class II companion protein as a molecule that can control the binding capacity of the fusion protein.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/23175356; hal-00965769; https://hal.science/hal-00965769Test; PUBMED: 23175356; PUBMEDCENTRAL: PMC3554189

الإتاحة: https://doi.org/10.1128/JVI.02659-12Test
https://hal.science/hal-00965769Test

المؤلفون: Dalmas, Olivier, Orelle, Cédric, Foucher, Anne-Emmanuelle, Geourjon, Christophe, Crouzy, Serge, Di Pietro, Attilio, Jault, Jean-Michel

المساهمون: Institut de biologie et chimie des protéines Lyon (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Department of Chemistry, Purdue University West Lafayette, Laboratoire de biophysique moléculaire et cellulaire (LBMC), Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 0021-9258.

مصطلحات موضوعية: MESH: ATP-Binding Cassette Transporters, MESH: Adenosine Triphosphatases, MESH: Disulfides, MESH: Escherichia coli, MESH: Membrane Transport Proteins, MESH: Models, Molecular, MESH: Molecular Sequence Data, MESH: Mutagenesis, Site-Directed, MESH: Mutation, MESH: Protein Conformation, MESH: Protein Folding, MESH: Protein Structure, Tertiary, MESH: Amino Acid Sequence, MESH: Sequence Homology, Amino Acid, MESH: Bacterial Proteins, MESH: Benzimidazoles, MESH: Binding Sites, MESH: Biological Transport, MESH: Catalysis, MESH: Cross-Linking Reagents, MESH: Dimerization, [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biophysics

الوصف: International audience ; The ATP-binding cassette is the most abundant family of transporters including many medically relevant members and gathers both importers and exporters involved in the transport of a wide variety of substrates. Although three high resolution three-dimensional structures have been obtained for a prototypic exporter, MsbA, two have been subjected to much criticism. Here, conformational changes of BmrA, a multidrug bacterial transporter structurally related to MsbA, have been studied. A three-dimensional model of BmrA, based on the "open" conformation of Escherichia coli MsbA, was probed by simultaneously introducing two cysteine residues, one in the first intracellular loop of the transmembrane domain and the other in the Q-loop of the nucleotide-binding domain (NBD). Intramolecular disulfide bonds could be created in the absence of any effectors, which prevented both drug transport and ATPase activity. Interestingly, addition of ATP/Mg plus vanadate strongly prevented this bond formation in a cysteine double mutant, whereas ATP/Mg alone was sufficient when the ATPase-inactive E504Q mutation was also introduced, in agreement with additional BmrA models where the ATP-binding sites are positioned at the NBD/NBD interface. Furthermore, cross-linking between the two cysteine residues could still be achieved in the presence of ATP/Mg plus vanadate when homobifunctional cross-linkers separated by more than 13 Angstrom were added. Altogether, these results give support to the existence, in the resting state, of a monomeric conformation of BmrA similar to that found within the open MsbA dimer and show that a large motion is required between intracellular loop 1 and the nucleotide-binding domain for the proper functioning of a multidrug ATP-binding cassette transporter.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/16107340; hal-00396916; https://hal.science/hal-00396916Test; PUBMED: 16107340

الإتاحة: https://doi.org/10.1074/jbc.M503266200Test
https://hal.science/hal-00396916Test

المؤلفون: Arrigo, André-Patrick, Firdaus, Wance J J, Mellier, Grégory, Moulin, Maryline, Paul, Catherine, Diaz-Latoud, Chantal, Kretz-Remy, Carole

المساهمون: Centre de génétique et de physiologie moléculaire et cellulaire (CGPhiMC), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)

المصدر: ISSN: 1046-2023.

مصطلحات موضوعية: MESH: Animals, MESH: Annexin A5, MESH: Coloring Agents, MESH: Cytochromes c, MESH: DNA, MESH: DNA Damage, MESH: Dimerization, MESH: Disulfides, MESH: Enzyme Activation, MESH: Flow Cytometry, MESH: Fluoresceins, MESH: Fluorescent Antibody Technique, Indirect, MESH: Antioxidants, MESH: Genetic Vectors, MESH: Gentian Violet, MESH: Glutathione, MESH: Heat-Shock Proteins, MESH: Hela Cells, MESH: Humans, MESH: Immunoblotting, MESH: Iron, MESH: Lipid Peroxidation, MESH: Mice, MESH: Apoptosis, MESH: Mitochondria, MESH: NIH 3T3 Cells, MESH: Neoplasm Proteins, MESH: Nitric Oxide, MESH: Oligonucleotides

الوصف: There is currently great interest in the development of methods to analyze intracellular redox state and the cellular damages generated by oxidative stress. General methods for analyzing reactive oxygen species and glutathione level are presented together with more recently developed protocols to analyze the consequences of oxidative stress on the oxidation of macromolecules. Finally, techniques to study modalities of constitutive expression of Hsp27 in mammalian cells are considered as well as methods used to determine the protective activity of this small heat shock protein against the deleterious effects induced by oxidative stress.

العلاقة: info:eu-repo/semantics/altIdentifier/pmid/15649839; hal-00193428; https://hal.science/hal-00193428Test; PUBMED: 15649839

الإتاحة: https://doi.org/10.1016/j.ymeth.2004.08.003Test
https://hal.science/hal-00193428Test