دورية أكاديمية
High genetic diversity revealed by variable-number tandem repeat genotyping and analysis of hsp65 gene polymorphism in a large collection of 'Mycobacterium canettii' strains indicates that the M. tuberculosis complex is a recently emerged clone of 'M. canettii'.
| العنوان: | High genetic diversity revealed by variable-number tandem repeat genotyping and analysis of hsp65 gene polymorphism in a large collection of 'Mycobacterium canettii' strains indicates that the M. tuberculosis complex is a recently emerged clone of 'M. canettii'. |
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| المؤلفون: | Fabre, Michel, Koeck, Jean-Louis, Le Flèche, Philippe, Simon, Fabrice, Hervé, Vincent, Vergnaud, Gilles, Pourcel, Christine |
| المساهمون: | Institut Camille Jordan (ICJ), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Jean Monnet - Saint-Étienne (UJM)-Centre National de la Recherche Scientifique (CNRS), Service de Bactériologie, Virologie, Hygiène CHU Limoges, CHU Limoges, Institut de génétique et microbiologie Orsay (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Pathologies Respiratoires : Protéolyse et Aérosolthérapie, HIA Percy, École Nationale Supérieure de Techniques Avancées (ENSTA Paris) |
| المصدر: | ISSN: 0095-1137 ; Journal of Clinical Microbiology ; https://ensta-paris.hal.science/hal-01158318Test ; Journal of Clinical Microbiology, 2004, 42 (7), pp.3248-55. ⟨10.1128/JCM.42.7.3248-3255.2004⟩. |
| بيانات النشر: | HAL CCSD American Society for Microbiology |
| سنة النشر: | 2004 |
| المجموعة: | HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: | MESH: Bacterial Proteins, MESH: Base Sequence, MESH: Molecular Sequence Data, MESH: Mycobacterium, MESH: Mycobacterium bovis, MESH: Mycobacterium tuberculosis, MESH: Polymorphism, Genetic, MESH: Repetitive Sequences, Nucleic Acid, MESH: Chaperonin 60, MESH: Chaperonins, MESH: Genetic Variation, MESH: Genotype, MESH: Minisatellite Repeats, [SDV]Life Sciences [q-bio], [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology |
| الوصف: | International audience ; We have analyzed, using complementary molecular methods, the diversity of 43 strains of "Mycobacterium canettii" originating from the Republic of Djibouti, on the Horn of Africa, from 1998 to 2003. Genotyping by multiple-locus variable-number tandem repeat analysis shows that all the strains belong to a single but very distant group when compared to strains of the Mycobacterium tuberculosis complex (MTBC). Thirty-one strains cluster into one large group with little variability and five strains form another group, whereas the other seven are more diverged. In total, 14 genotypes are observed. The DR locus analysis reveals additional variability, some strains being devoid of a direct repeat locus and others having unique spacers. The hsp65 gene polymorphism was investigated by restriction enzyme analysis and sequencing of PCR amplicons. Four new single nucleotide polymorphisms were discovered. One strain was characterized by three nucleotide changes in 441 bp, creating new restriction enzyme polymorphisms. As no sequence variability was found for hsp65 in the whole MTBC, and as a single point mutation separates M. tuberculosis from the closest "M. canettii" strains, this diversity within "M. canettii" subspecies strongly suggests that it is the most probable source species of the MTBC rather than just another branch of the MTBC. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/15243089; hal-01158318; https://ensta-paris.hal.science/hal-01158318Test; https://ensta-paris.hal.science/hal-01158318/documentTest; https://ensta-paris.hal.science/hal-01158318/file/Fabre2004JCM.pdfTest; PUBMED: 15243089; PUBMEDCENTRAL: PMC446256 |
| DOI: | 10.1128/JCM.42.7.3248-3255.2004 |
| الإتاحة: | https://doi.org/10.1128/JCM.42.7.3248-3255.2004Test https://ensta-paris.hal.science/hal-01158318Test https://ensta-paris.hal.science/hal-01158318/documentTest https://ensta-paris.hal.science/hal-01158318/file/Fabre2004JCM.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.E77F6D85 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1128/JCM.42.7.3248-3255.2004 |
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