دورية أكاديمية
Tissue kallikrein is required for the cardioprotective effect of Cyclosporin A in myocardial ischemia in the mouse
| العنوان: | Tissue kallikrein is required for the cardioprotective effect of Cyclosporin A in myocardial ischemia in the mouse |
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| المؤلفون: | Youcef, G., Belaidi, Elise, Waeckel, L., Fazal, L., Clemessy, M., Vincent, M.P., Zadigue, G., Richer, C., Alhenc-Gelas, F., Ovize, Michel, Pizard, A. |
| المساهمون: | Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Défaillance Cardiovasculaire Aiguë et Chronique (DCAC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Centre d'investigation clinique Nancy (CIC) |
| المصدر: | ISSN: 0006-2952. |
| بيانات النشر: | HAL CCSD Elsevier |
| سنة النشر: | 2015 |
| المجموعة: | HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: | Cardiac ischemia–reperfusion, Cyclosporin A, Mitochondria, Tissue kallikrein, MESH: Animals, MESH: Cardiotonic Agents, MESH: Ischemic Postconditioning, MESH: Male, MESH: Mice, Knockout, MESH: Mitochondria, Heart, MESH: Mitochondrial Membrane Transport Proteins, MESH: Myocardial Ischemia, MESH: Myocardium, MESH: Myocytes, Cardiac, MESH: Oxidative Phosphorylation, MESH: Signal Transduction, MESH: Rats, MESH: Tissue Kallikreins, MESH: Cyclophilins, MESH: Voltage-Dependent Anion Channel 1, MESH: Cyclosporine, MESH: Gene Expression, MESH: Heterozygote, MESH: Homozygote, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system |
| الوصف: | International audience ; Clinical and experimental studies suggest that pharmacological postconditioning with Cyclosporin A (CsA) reduces infarct size in cardiac ischemia and reperfusion. CsA interacts with Cyclophilin D (CypD) preventing opening of the mitochondrial permeability transition pore (mPTP). Tissue kallikrein (TK) and its products kinins are involved in cardioprotection in ischemia. CypD knockout mice are resistant to the cardioprotective effects of both CsA and kinins suggesting common mechanisms of action. Using TK gene knockout mice, we investigated whether the kallikrein-kinin system is involved in the cardioprotective effect of CsA. Homozygote and heterozygote TK deficient mice (TK(-/-), TK(+/-)) and wild type littermates (TK(+/+)) were subjected to cardiac ischemia-reperfusion with and without CsA postconditioning. CsA reduced infarct size in TK(+/+) mice but had no effect in TK(+/-) and TK(-/-) mice. Cardiac mitochondria isolated from TK(-/-) mice had indistinguishable basal oxidative phosphorylation and calcium retention capacity compared to TK(+/+) mice but were resistant to CsA inhibition of mPTP opening. TK activity was documented in mouse heart and rat cardiomyoblasts mitochondria. By proximity ligation assay TK was found in close proximity to the mitochondrial membrane proteins VDAC and Tom22, and CypD. Thus, partial or total deficiency in TK induces resistance to the infarct size reducing effect of CsA in cardiac ischemia in mice, suggesting that TK level is a critical factor for cardioprotection by CsA. TK is required for the mitochondrial action of CsA and may interact with CypD. Genetic variability in TK activity has been documented in man and may influence the cardioprotective effect of CsA. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/25623731; hal-01758765; https://hal.univ-lorraine.fr/hal-01758765Test; PUBMED: 25623731 |
| DOI: | 10.1016/j.bcp.2015.01.007 |
| الإتاحة: | https://doi.org/10.1016/j.bcp.2015.01.007Test https://hal.univ-lorraine.fr/hal-01758765Test |
| رقم الانضمام: | edsbas.419F6928 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.bcp.2015.01.007 |
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