دورية أكاديمية
Postconditioning with cyclosporine a reduces early renal dysfunction by inhibiting mitochondrial permeability transition
| العنوان: | Postconditioning with cyclosporine a reduces early renal dysfunction by inhibiting mitochondrial permeability transition |
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| المؤلفون: | Lemoine, Sandrine, Pillot, B., Rognant, N., Augeul, L., Rayberin, M., Varennes, A., Laville, M., Ovize, Michel, Juillard, Laurent |
| المساهمون: | Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), ANR-10-IBHU-0004,OPeRa,Organ ProtEction and ReplAcement(2010) |
| المصدر: | ISSN: 0041-1337. |
| بيانات النشر: | HAL CCSD Lippincott, Williams & Wilkins |
| سنة النشر: | 2015 |
| المجموعة: | HAL Lyon 1 (University Claude Bernard Lyon 1) |
| مصطلحات موضوعية: | Cyclosporine/*administration & dosage, Models, Mice, Urea/blood, Apoptosis/drug effects, Animals, Time Factors, Male, Biomarkers/blood, Mitochondrial Membrane Transport Proteins/*antagonists & inhibitors/metabolism, Cytoprotection, Oxidative Phosphorylation/drug effects, Creatinine/blood, Inbred BALB C, Immunosuppressive Agents/*administration & dosage, Kidney Transplantation/adverse effects/*methods, Ischemic Postconditioning/*methods, Kidney/*drug effects/metabolism/pathology/physiopathology/*surgery, Mitochondria/*drug effects/metabolism/pathology, Nephrectomy, Reperfusion Injury/metabolism/pathology/physiopathology/*prevention & control, Animal, [SDV]Life Sciences [q-bio] |
| الوصف: | International audience ; BACKGROUND: Ischemia-reperfusion (IR) injury leads to mitochondrial permeability transition pore opening, which contributes to cell death. The aim of this study is to determine whether ischemic or pharmacological postconditioning with cyclosporine A (CsA) might protect the kidney from lethal reperfusion injury. METHODS: Male mice underwent a unilateral (right) nephrectomy followed by 30 minutes of contralateral (left) clamping of the renal artery. We studied 4 groups at 20 minutes and 24 hours of reperfusion: a sham group (n = 4), an ischemic group (n = 6), CsA-postconditioned group (postcond-CsA, injection of 3 mg/kg of CsA 5 minutes before the end of ischemia, (n = 6), and an ischemic postconditioning (IPC) group (n = 6), consisting of 3 cycles of 30 seconds of renal ischemia with 30 seconds intervening reperfusion. After 24 hours of reperfusion, we measured plasma creatinine, urea, and histological kidney injury. The kidney mitochondria were isolated to assess the mitochondria calcium retention capacity and oxidative phosphorylation. RESULTS: At 24 hours after reperfusion, serum creatinine decreased in postcond-CsA and IPC compared to ischemic group. The histological score was also significantly improved with postcond-CsA and IPC. At 20 minutes and 24 hours of reperfusion, calcium retention capacity was decreased significantly in the ischemic group. The mitochondrial respiration stay decreased in the ischemic group at 24 hours of reperfusion, whereas the respiration was improved significantly in the postcond-CsA and IPC group. Bax and cleaved caspase 3 decreased in PostCsA and IPC group. CONCLUSIONS: Our results suggest that IPC and CsA, administered immediately before reperfusion, protect the kidney from lethal injury. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | hal-01850624; https://hal.science/hal-01850624Test |
| DOI: | 10.1097/TP.0000000000000530 |
| الإتاحة: | https://doi.org/10.1097/TP.0000000000000530Test https://hal.science/hal-01850624Test |
| رقم الانضمام: | edsbas.C72C2D39 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1097/TP.0000000000000530 |
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