دورية أكاديمية
Dual Role Of Gnaa In Antibiotic Resistance And Virulence In Acinetobacter Baumannii
العنوان: | Dual Role Of Gnaa In Antibiotic Resistance And Virulence In Acinetobacter Baumannii |
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المؤلفون: | Xu, Qingye, Chen, Tao, Yan, Biyong, Zhang, Linyue, Pi, Borui, Yang, Yunxing, Zhang, Linghong, Zhou, Zhihui, Ji, Shujuan, Leptihn, Sebastian, Akova, Murat, Yu, Yunsong, Hua, Xiaoting |
المساهمون: | İç Hastalıkları |
سنة النشر: | 2019 |
المجموعة: | Hacettepe University Institutional Repository |
الوصف: | Acinetobacter baumannii is an important Gram-negative pathogen in hospital-related infections. However, treatment options for A. baumannii infections have become limited due to multidrug resistance. Bacterial virulence is often associated with capsule genes found in the K locus, many of which are essential for biosynthesis of the bacterial envelope. However, the roles of other genes in the K locus remain largely unknown., Acinetobacter baumannii is an important Gram-negative pathogen in hospital-related infections. However, treatment options for A. baumannii infections have become limited due to multidrug resistance. Bacterial virulence is often associated with capsule genes found in the K locus, many of which are essential for biosynthesis of the bacterial envelope. However, the roles of other genes in the K locus remain largely unknown. From an in vitro evolution experiment, we obtained an isolate of the virulent and multidrug-resistant A. baumannii strain MDR-ZJ06, called MDR-ZJ06M, which has an insertion by the ISAba16 transposon in gnaA (encoding UDP-N-acetylglucosamine C-6 dehydrogenase), a gene found in the K locus. The isolate showed an increased resistance toward tigecycline, whereas the MIC decreased in the case of carbapenems, cephalosporins, colistin, and minocycline. By using knockout and complementation experiments, we demonstrated that gnaA is important for the synthesis of lipooligosaccharide and capsular polysaccharide and that disruption of the gene affects the morphology, drug susceptibility, and virulence of the pathogen. |
نوع الوثيقة: | article in journal/newspaper |
وصف الملف: | application/pdf |
اللغة: | English |
تدمد: | 0066-4804 |
العلاقة: | Antimicrobial Agents And Chemotherapy; http://dx.doi.org/10.1128/AAC.00694-19Test; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761518Test/; http://hdl.handle.net/11655/23956Test; 63; 10 |
DOI: | 10.1128/AAC.00694-19 |
الإتاحة: | https://doi.org/10.1128/AAC.00694-19Test http://hdl.handle.net/11655/23956Test https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6761518Test/ |
حقوق: | Attribution 4.0 United States ; info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/4.0Test/ |
رقم الانضمام: | edsbas.AA7BCCA2 |
قاعدة البيانات: | BASE |
تدمد: | 00664804 |
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DOI: | 10.1128/AAC.00694-19 |