المؤلفون: Persyn, Antoine, García Méndez, Sonia, De Meyer, Sam, Beirinckx, Stien, Willems, Anne, De Tender, Caroline, Goormachtig, Sofie

المصدر: PHYTOBIOMES JOURNAL ; ISSN: 2471-2906

مصطلحات موضوعية: Biology and Life Sciences, microbiome, lettuce, PGPB, chilling temperatures, root endosphere, plant growth-promoting rhizobacteria

الوصف: Growth of lettuce (Lactuca sativa) is severely hampered by low temperatures, even when cultivated under greenhouse conditions. Root-associated bacteria might promote plant growth under stressful conditions. Therefore, we analyzed the effect of low temperatures on the lettuce root-associated microbiome to evaluate whether microbiome-based selection aids in the identification of bacteria that stimulate plant growth in the cold. 16S rRNA gene amplicon sequencing was used to examine the compositional differences in the lettuce root-associated microbiome when grown under low and control temperature conditions. Chilling temperatures significantly altered the lettuce root endosphere composition, whereas its effects were less severe in the rhizosphere and absent in the bulk soil. Several cold-enriched families were found, of which nine, the Oxalobacteraceae, Pseudomonadaceae, Flavobacteriaceae, Microscillaceae, Spingobacteriaceae, Comamonadaceae, Devosiaceae, Methylophilaceae and env.OPS_17, in both the rhizosphere and the root endosphere. Concurrently, a collection of lettuce root-colonizing bacteria was established and based on correlation with these families, representative isolates were screened. None of the lettuce root isolates showed growth-promoting effects, but three growth-promoting Flavobacterium strains from an available collection of grass root-colonizing bacteria were identified. Amplicon sequence variant (ASV) annotation of the lettuce and grass strains revealed that strains matching cold-enriched or highly abundant ASVs in at least one soil promoted growth in the cold. Overall our data demonstrate that microbiome analyses, combined with high-throughput bacterial isolations, might be a helpful tool to isolate effective cold growth-promoting strains.

وصف الملف: application/zip

العلاقة: https://biblio.ugent.be/publication/01GKV10HBMC7GFZ6N6F9T8E92NTest; http://hdl.handle.net/1854/LU-01GKV10HBMC7GFZ6N6F9T8E92NTest; http://doi.org/10.1094/PBIOMES-07-22-0044-MFTest; https://biblio.ugent.be/publication/01GKV10HBMC7GFZ6N6F9T8E92N/file/01GKV42PF44FQAP41P6T6WMWEYTest

الإتاحة: https://doi.org/10.1094/PBIOMES-07-22-0044-MFTest
https://biblio.ugent.be/publication/01GKV10HBMC7GFZ6N6F9T8E92NTest
http://hdl.handle.net/1854/LU-01GKV10HBMC7GFZ6N6F9T8E92NTest
https://biblio.ugent.be/publication/01GKV10HBMC7GFZ6N6F9T8E92N/file/01GKV42PF44FQAP41P6T6WMWEYTest

المؤلفون: Van Gerrewey, Thijs, Boon, Nico, Geelen, Danny

المصدر: AGRONOMY-BASEL ; ISSN: 2073-4395

مصطلحات موضوعية: Agriculture and Food Sciences, vertical farming, growing media, rhizosphere, microbiome, plant factory, sustainability, PGPR, plant growth-promoting rhizobacteria, GHG, water use efficiency, BACTERIAL ROOT MICROBIOTA, RHIZOSPHERE MICROBIOME, PLANT FACTORIES, SYSTEMS, CULTIVATION, DIVERSITY, DYNAMICS, LETTUCE, FIELD

الوصف: Vertical farming is on its way to becoming an addition to conventional agricultural practices, improving sustainable food production for the growing world population under increasing climate stress. While the early development of vertical farming systems mainly focused on technological advancement through design innovation, the automation of hydroponic cultivation, and advanced LED lighting systems, more recent studies focus on the resilience and circularity of vertical farming. These sustainability objectives are addressed by investigating water quality and microbial life in a hydroponic cultivation context. Plant growth-promoting rhizobacteria (PGPR) have been shown to improve plant performance and resilience to biotic and abiotic stresses. The application of PGPRs to plant-growing media increases microbial functional diversity, creating opportunities to improve the circularity and resilience of vertical farming systems by reducing our dependency on chemical fertilizers and crop protection products. Here, we give a brief historical overview of vertical farming, review its opportunities and challenges in an economic, environmental, social, and political context, and discuss advances in exploiting the rhizosphere microbiome in hydroponic cultivation systems.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8739057Test; http://hdl.handle.net/1854/LU-8739057Test; http://dx.doi.org/10.3390/agronomy12010002Test; https://biblio.ugent.be/publication/8739057/file/8739059Test

الإتاحة: https://doi.org/10.3390/agronomy12010002Test
https://biblio.ugent.be/publication/8739057Test
http://hdl.handle.net/1854/LU-8739057Test
https://biblio.ugent.be/publication/8739057/file/8739059Test

المؤلفون: Andric, Sofija, Meyer, Thibault, Rigolet, Augustin, Prigent-Combaret, Claire, Höfte, Monica, Balleux, Guillaume, Steels, Sebastien, Hoff, Gregory, De Mot, Rene, McCann, Andrea, De Pauw, Edwin, Arias, Anthony Argueelles, Ongena, Marc

المصدر: MICROBIOLOGY SPECTRUM ; ISSN: 2165-0497

مصطلحات موضوعية: Biology and Life Sciences, Bacillus velezensis, molecular cross-talk, Pseudomonas, bioactive secondary metabolites, cyclic lipopeptides, microbial ecology, microbial interaction, plant growth-promoting rhizobacteria, NATURAL-PRODUCTS, METABOLIC EXCHANGE, SUBTILIS, SURFACTANTS, PHENAZINES, RESISTANCE, DIVERSITY, INSIGHTS, ROLES, FORM

الوصف: Some Bacillus species, such as B. velezensis, are important members of the plant-associated microbiome, conferring protection against phytopathogens. However, our knowledge about multitrophic interactions determining the ecological fitness of these biocontrol bacteria in the competitive rhizosphere niche is still limited. Here, we investigated molecular mechanisms underlying interactions between B. velezensis and Pseudomonas as a soil-dwelling competitor. Upon their contact-independent in vitro confrontation, a multifaceted macroscopic outcome was observed and characterized by Bacillus growth inhibition, white line formation in the interaction zone, and enhanced motility. We correlated these phenotypes with the production of bioactive secondary metabolites and identified specific lipopeptides as key compounds involved in the interference interaction and motile response. Bacillus mobilizes its lipopeptide surfactin not only to enhance motility but also to act as a chemical trap to reduce the toxicity of lipopeptides formed by Pseudomonas. We demonstrated the relevance of these unsuspected roles of lipopeptides in the context of competitive tomato root colonization by the two bacterial genera. IMPORTANCE: Plant-associated Bacillus velezensis and Pseudomonas spp. represent excellent model species as strong producers of bioactive metabolites involved in phytopathogen inhibition and the elicitation of plant immunity. However, the ecological role of these metabolites during microbial interspecies interactions and the way their expression may be modulated under naturally competitive soil conditions has been poorly investigated. Through this work, we report various phenotypic outcomes from the interactions between B. velezensis and 10 Pseudomonas strains used as competitors and correlate them with the production of specific metabolites called lipopeptides from both species. More precisely, Bacillus overproduces surfactin to enhance motility, which also, by acting as a chemical trap, reduces the toxicity of other ...

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8745033Test; http://hdl.handle.net/1854/LU-8745033Test; http://dx.doi.org/10.1128/spectrum.02038-21Test; https://biblio.ugent.be/publication/8745033/file/8745043Test

الإتاحة: https://doi.org/10.1128/spectrum.02038-21Test
https://biblio.ugent.be/publication/8745033Test
http://hdl.handle.net/1854/LU-8745033Test
https://biblio.ugent.be/publication/8745033/file/8745043Test

المؤلفون: Van Gerrewey, Thijs, Vandecruys, Maarten, Ameloot, Nele, Perneel, Maaike, Van Labeke, Marie-Christine, Boon, Nico, Geelen, Danny

المصدر: AGRONOMY-BASEL ; ISSN: 2073-4395

مصطلحات موضوعية: Agriculture and Food Sciences, plant growth-promoting rhizobacteria (PGPR), growing media, rhizosphere, lettuce, plant factory, soilless culture, plant quality, plant yield, microbiome, beneficial bacteria, GROWTH-PROMOTING RHIZOBACTERIA, ARBUSCULAR MYCORRHIZAL FUNGI, PHYSICAL-PROPERTIES, BIOCHAR ADDITION, SYNERGISTIC USE, FUSARIUM-WILT, BROWN PEAT, BACILLUS, INOCULATION

الوصف: There is a need for plant growing media that can support a beneficial microbial root environment to ensure that optimal plant growth properties can be achieved. We investigated the effect of five rhizosphere bacterial community inocula (BCI S1-5) that were collected at three open field organic farms and two soilless farms on the performance of lettuce (Lactuca sativa L.). The lettuce plants were grown in ten different plant growing media (M1-10) composed of 60% v/v peat (black peat or white peat), 20% v/v other organics (coir pith or wood fiber), 10% v/v composted materials (composted bark or green waste compost) and 10% v/v inorganic materials (perlite or sand), and one commercial plant growing medium inside a plant factory with artificial lighting. Fractional factorial design of experiments analysis revealed that the bacterial community inoculum, plant growing medium composition, and their interaction determine plant performance. The impact of bacterial amendments on the plant phenotype relied on the bacterial source. For example, S3 treatment significantly increased lettuce shoot fresh weight (+57%), lettuce head area (+29%), root fresh weight (+53%), and NO3-content (+53%), while S1 treatment significantly increased lettuce shoot dry weight (+15%), total phenolic content (+65%), and decreased NO3-content (-67%). However, the effectiveness of S3 and S1 treatment depended on plant growing medium composition. Principal component analysis revealed that shoot fresh weight, lettuce head area, root fresh weight, and shoot dry weight were the dominant parameters contributing to the variation in the interactions. The dominant treatments were S3-M8, S1-M7, S2-M4, the commercial plant growing medium, S1-M2, and S3-M10. Proper selection of plant growing medium composition is critical for the efficacy of bacterial amendments and achieving optimal plant performance inside a plant factory with artificial lighting.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8681303Test; http://hdl.handle.net/1854/LU-8681303Test; http://dx.doi.org/10.3390/agronomy10101456Test; https://biblio.ugent.be/publication/8681303/file/8681304Test

الإتاحة: https://doi.org/10.3390/agronomy10101456Test
https://biblio.ugent.be/publication/8681303Test
http://hdl.handle.net/1854/LU-8681303Test
https://biblio.ugent.be/publication/8681303/file/8681304Test

المؤلفون: Beirinckx, Stien, Viaene, Tom, Haegeman, Annelies, Debode, Jane, Amery, Fien, Vandenabeele, Steven, Nelissen, Hilde, Inzé, Dirk, Tito, Raul, Raes, Jeroen, De Tender, Caroline, Goormachtig, Sofie

المصدر: MICROBIOME ; ISSN: 2049-2618

مصطلحات موضوعية: Biology and Life Sciences, Chilling temperatures, Maize, Microbiome, PGPR, Plant growth-promoting rhizobacteria, Root endosphere, Burkholderia-phytofirmans-psjn, Colonization, Drought, Stress, Tolerance, Cold, Selection, Strains, Impact

الوصف: Background When maize (Zea mays L.) is grown in the Northern hemisphere, its development is heavily arrested by chilling temperatures, especially at the juvenile phase. As some endophytes are beneficial for plants under stress conditions, we analyzed the impact of chilling temperatures on the root microbiome and examined whether microbiome-based analysis might help to identify bacterial strains that could promote growth under these temperatures. Results We investigated how the maize root microbiome composition changed by means of 16S rRNA gene amplicon sequencing when maize was grown at chilling temperatures in comparison to ambient temperatures by repeatedly cultivating maize in field soil. We identified 12 abundant and enriched bacterial families that colonize maize roots, consisting of bacteria recruited from the soil, whereas seed-derived endophytes were lowly represented. Chilling temperatures modified the root microbiome composition only slightly, but significantly. An enrichment of several chilling-responsive families was detected, of which the Comamonadaceae and the Pseudomonadaceae were the most abundant in the root endosphere of maize grown under chilling conditions, whereas only three were strongly depleted, among which the Streptomycetaceae. Additionally, a collection of bacterial strains isolated from maize roots was established and a selection was screened for growth-promoting effects on juvenile maize grown under chilling temperatures. Two promising strains that promoted maize growth under chilling conditions were identified that belonged to the root endophytic bacterial families, from which the relative abundance remained unchanged by variations in the growth temperature. Conclusions Our analyses indicate that chilling temperatures affect the bacterial community composition within the maize root endosphere. We further identified two bacterial strains that boost maize growth under chilling conditions. Their identity revealed that analyzing the chilling-responsive families did not help for their ...

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8659266Test; http://hdl.handle.net/1854/LU-8659266Test; http://dx.doi.org/10.1186/s40168-020-00833-wTest; https://biblio.ugent.be/publication/8659266/file/8659267Test

الإتاحة: https://doi.org/10.1186/s40168-020-00833-wTest
https://biblio.ugent.be/publication/8659266Test
http://hdl.handle.net/1854/LU-8659266Test
https://biblio.ugent.be/publication/8659266/file/8659267Test

المؤلفون: García Méndez, Sonia, Persyn, Antoine, De Tender, Caroline, Goormachtig, Sofie, Willems, Anne

المصدر: PHYTOBIOMES JOURNAL ; ISSN: 2471-2906

مصطلحات موضوعية: Biology and Life Sciences, alerianella locusta, root endosphere, microbiome, cold stress, chilling temperatures, Plant growth-promoting rhizobacteria

الوصف: Low temperatures greatly affect plant growth. Besides the development of own protection mechanisms, plants may rely on microorganisms to help them cope with cold. As Valerianella locusta (lamb’s lettuce) is a cold-adapted plant, it represents an interesting plant to understand how cold affects the root bacteriome. By means of amplicon sequencing of 16S rRNA genes, we identified the taxa forming the main cold root bacteriome of lamb’s lettuce. The analysis of the root microbiome of a naturally growing V. locusta population and the study of the changes in the root microbiome of cultivated V. locusta grown under cold and ambient conditions allowed us to detect four cold-responsive families, of which Oxalobacteraceae presented the strongest shift under chilling temperatures. Moreover, the plant genotype had a small, but significant, effect on the response of the root bacteriome to cold. Finally, several bacterial candidates were discovered that may possibly alleviate the effect of low temperatures on plant fitness, namely Massilia ASV2, Flavobacterium ASV5 and ASV11, or Acidovorax ASV20. However, currently, only ten cold-enriched isolates could be obtained in cultivation, of which Flavobacterium R-83141, Polaromonas R-83176, R-83177, R-83175 and Acidovorax R-83129, were able to increase either the root, shoot and/or total fresh weight of a cold sensitive ecotype of A. thaliana (Cvi-0). Our results provide an overview of the taxa forming the root microbiome of this species and of the compositional shift that occurs under cold temperature treatment, demonstrating the impact of low temperatures on the composition of the root microbiome.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/01H5VCTEQPZFN4M1JW2A7Y34Y7Test; http://hdl.handle.net/1854/LU-01H5VCTEQPZFN4M1JW2A7Y34Y7Test; http://doi.org/10.1094/pbiomes-12-22-0106-rTest; https://biblio.ugent.be/publication/01H5VCTEQPZFN4M1JW2A7Y34Y7/file/01H5VD4YEZYRWV4T0VR4DJRG2DTest

الإتاحة: https://doi.org/10.1094/pbiomes-12-22-0106-rTest
https://biblio.ugent.be/publication/01H5VCTEQPZFN4M1JW2A7Y34Y7Test
http://hdl.handle.net/1854/LU-01H5VCTEQPZFN4M1JW2A7Y34Y7Test
https://biblio.ugent.be/publication/01H5VCTEQPZFN4M1JW2A7Y34Y7/file/01H5VD4YEZYRWV4T0VR4DJRG2DTest

المؤلفون: Si, Jiyeon, froussart, Emilie, Viaene, Tom, Vázquez-Castellanos, Jorge F., Hamonts, Kelly, Tang, Lin, Beirinckx, Stien, De Keyser, Annick, Deckers, Tibby, Amery, Fien, Vandenabeele, Steven, Raes, Jeroen, Goormachtig, Sofie

المصدر: COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL ; ISSN: 2001-0370

مصطلحات موضوعية: Biology and Life Sciences, Agriculture and Food Sciences, Drought stress, Soil compositions, Soil microbiome, Endosphere microbiome, Plant Growth-Promoting Rhizobacteria (PGPR), Actinobacteria, Streptomyces isolates, β-glucosidase, osmoprotectant proteins, amplicon sequence variants (ASVs), BACTERIAL COMMUNITY, FUNGAL COMMUNITIES, TRITICUM-AESTIVUM, TOLERANCE, GROWTH, PHOTOSYNTHESIS, RESPONSES, SALT, PH

الوصف: As wheat (Triticum aestivum) is an important staple food across the world, preservation of stable yields and increased productivity are major objectives in breeding programs. Drought is a global concern because its adverse impact is expected to be amplified in the future due to the current climate change. Here, we analyzed the effects of edaphic, environmental, and host factors on the wheat root microbiomes collected in soils from six regions in Belgium. Amplicon sequencing analysis of unplanted soil and wheat root endosphere samples indicated that the microbial community variations can be significantly explained by soil pH, microbial biomass, wheat genotype, and soil sodium and iron levels. Under drought stress, the biodiversity in the soil decreased significantly, but increased in the root endosphere community, where specific soil parameters seemingly determine the enrichment of bacterial groups. Indeed, we identified a cluster of drought-enriched bacteria that significantly correlated with soils compositions. Interestingly, integration of a functional analysis further revealed a strong correlation between the same cluster of bacteria and β-glucosidase and osmoprotectant proteins, two functions known to be involved in coping with drought stress. By means of this in silico analysis, we identified amplicon sequence variants (ASVs) that could potentially protect the plant from drought stress and validated them in planta. Yet, ASVs based on 16S rRNA sequencing data did not completely distinguish individual isolates because of their intrinsic short sequences. Our findings support the efforts to maintain stable crop yields under drought conditions through implementation of root microbiome analyses.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/8716684Test; http://hdl.handle.net/1854/LU-8716684Test; http://dx.doi.org/10.1016/j.csbj.2021.07.027Test; https://biblio.ugent.be/publication/8716684/file/8716685Test

الإتاحة: https://doi.org/10.1016/j.csbj.2021.07.027Test
https://biblio.ugent.be/publication/8716684Test
http://hdl.handle.net/1854/LU-8716684Test
https://biblio.ugent.be/publication/8716684/file/8716685Test

المؤلفون: De Meyer, Geert, Höfte, Monica

المصدر: PHYTOPATHOLOGY ; ISSN: 0031-949X

مصطلحات موضوعية: Biology and Life Sciences, induced systemic resistance, Phaseolus vulgaris, plant growth-promoting rhizobacteria, reporter gene, SP STRAIN WCS417R, SYSTEMIC RESISTANCE, FUSARIUM-WILT, ACQUIRED-RESISTANCE, DISEASE RESISTANCE, RHIZOSPHERE, FLUORESCENS, INDUCTION, BACTERIA, TOBACCO

الوصف: Selected strains of nonpathogenic rhizobacteria can induce a systemic resistance in plants that is effective against various pathogens. In an assay with bean plants, we investigated which determinants of the rhizobacterium Pseudomonas aeruginosa 7NSK2 are important for induction of resistance to Botrytis cinerea. By varying the iron nutritional state of the bacterium at inoculation, it was demonstrated that induced resistance by P. aeruginosa 7NSK2 was iron-regulated. As P. aeruginosa 7NSK2 produces three siderophores under iron limitation, pyoverdin, pyochelin, and salicylic acid, we investigated the involvement of these iron-regulated metabolites in induced resistance by using mutants deficient in one or more siderophores. Results demonstrated that salicylic acid production was essential for induction of resistance to B. cinerea by Fl aeruginosa 7NSK2 in bean and did not exclude a role for pyochelin. A role for pyoverdin, however, could not be demonstrated. Transcriptional activity of salicylic acid and pyochelin biosynthetic genes was detected during P. aeruginosa 7NSK2 colonization of bean. Moreover, the iron nutritional state at inoculation influenced the transcriptional activity of salicylic acid and pyochelin biosynthetic genes in the same way as it influenced induction of systemic resistance to B. cinerea.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/184429Test; http://hdl.handle.net/1854/LU-184429Test; http://dx.doi.org/10.1094/PHYTO.1997.87.6.588Test; https://biblio.ugent.be/publication/184429/file/4305576Test

الإتاحة: https://doi.org/10.1094/PHYTO.1997.87.6.588Test
https://biblio.ugent.be/publication/184429Test
http://hdl.handle.net/1854/LU-184429Test
https://biblio.ugent.be/publication/184429/file/4305576Test

المؤلفون: Schelfhout, Stephanie, De Schrijver, An, De Bolle, Sara, De Gelder, Leen, Demey, Andreas, Du Pré, Tom, De Neve, Stefaan, Haesaert, Geert, Verheyen, Kris, Mertens, Jan

المصدر: RESTORATION ECOLOGY ; ISSN: 1061-2971

مصطلحات موضوعية: Earth and Environmental Sciences, humic substances, nardus grassland, phosphate-solubilizing bacteria, phytoextraction, arbuscular mycorrhiza, plant-growth-promoting rhizobacteria, ARBUSCULAR-MYCORRHIZAL FUNGI, PLANT-SPECIES LOSS, SOIL-PHOSPHORUS, NUTRIENT-UPTAKE, HUMIC-ACID, FERTILIZER APPLICATION, NITROGEN ENRICHMENT, EUROPEAN GRASSLANDS, CROP YIELD

الوصف: To restore species-rich terrestrial ecosystems on ex-agricultural land, establishing nutrient limitation for dominant plant growth is essential, because in nutrient-rich soils, fast-growing species often exclude target species. However N-limitation is easier to achieve than P-limitation (because of a difference in biogeochemical behavior), biodiversity is generally highest under P-limitation. Commonly-used restoration methods to achieve low soil P-concentrations are either very expensive or take a very long time. A promising restoration technique is P-mining, an adjusted agricultural technique that aims at depleting soil-P. High biomass production and hence high P-removal with biomass is obtained by fertilizing with nutrients other than P. A pot experiment was set up to study P-mining with Lolium perenne L. on sandy soils with varying P-concentrations: from an intensively-used agricultural soil to a soil near the soil P-target for species-rich Nardus grassland. All pots received N- and K-fertilization. The effects of biostimulants on P-uptake were also assessed by the addition of arbuscular mycorrhiza (Glomus spp.), humic substances or phosphate-solubilizing bacteria (Bacillus sp. and Pseudomonas spp.). In our P-rich soil (111 μg POlsen/g), P-removal rate was high but bioavailable soil-P did not decrease. At lower soil P-concentrations (64 and 36 μg POlsen/g), bioavailable soil-P had decreased but the P-removal rate had by then dropped 60% despite N- and K-fertilization and despite that the target (< 10 μg POlsen/g) was still far away. None of the biostimulants altered this trajectory. Therefore, restoration will still take decades when starting with ex-agricultural soils unless P-fertilization history was much lower than average.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/6979097Test; http://hdl.handle.net/1854/LU-6979097Test; http://dx.doi.org/10.1111/rec.12264Test; https://biblio.ugent.be/publication/6979097/file/6979098Test

الإتاحة: https://doi.org/10.1111/rec.12264Test
https://biblio.ugent.be/publication/6979097Test
http://hdl.handle.net/1854/LU-6979097Test
https://biblio.ugent.be/publication/6979097/file/6979098Test

المؤلفون: De Meyer, Geert, Audenaert, Kris, Höfte, Monica

المصدر: EUROPEAN JOURNAL OF PLANT PATHOLOGY ; ISSN: 0929-1873

مصطلحات موضوعية: Biology and Life Sciences, TRANSLOCATED SIGNAL, BIOCONTROL BACTERIA, GENE-EXPRESSION, INDUCTION, ARABIDOPSIS, CUCUMBER, FLUORESCENS, INFECTION, NECROSIS VIRUS, ACQUIRED-RESISTANCE, systemic acquired resistance, plant growth-promoting rhizobacteria

الوصف: Root colonization by rhizobacteria can induce a systemic resistance in plants that is phenotypically similar to systemic acquired resistance induced by a localized pathogen infection. We used the tobacco-tobacco mosaic virus model to investigate whether the systemic resistance induced by the rhizobacterium Pseudomonas aeruginosa 7NSK2 is mediated by the systemic acquired resistance signal transduction pathway. Experiments with nahG-transformed tobacco revealed that Pseudomonas aeruginosa 7NSK2-induced resistance depended on in planta salicylic acid accumulation for its expression but not for its induction and is, in this respect, similar to systemic acquired resistance. However, Pseudomonas aeruginosa 7NSK2-induced resistance was, unlike systemic acquired resistance, not associated with PR1a expression at the time of challenge with tobacco mosaic virus. This suggests that Pseudomonas aeruginosa 7NSK2 treatment would only potentiate defense gene expression in systemic tissue, which would also explain why its level of resistance is lower than in case of systemic acquired resistance. Because we demonstrated that induced resistance by Pseudomonas aeruginosa 7NSK2 exclusively depends on the production of salicylic acid by this strain our conclusions might also account for other salicylic acid-producing and resistance-inducing rhizobacteria.

وصف الملف: application/pdf

العلاقة: https://biblio.ugent.be/publication/119927Test; http://hdl.handle.net/1854/LU-119927Test; http://dx.doi.org/10.1023/A:1008741015912Test; https://biblio.ugent.be/publication/119927/file/4304922Test

الإتاحة: https://doi.org/10.1023/A:1008741015912Test
https://biblio.ugent.be/publication/119927Test
http://hdl.handle.net/1854/LU-119927Test
https://biblio.ugent.be/publication/119927/file/4304922Test