دورية أكاديمية
A timer for analyzing temporally dynamic changes in transcription during differentiation in vivo
| العنوان: | A timer for analyzing temporally dynamic changes in transcription during differentiation in vivo |
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| المؤلفون: | Bending, David, Martin, Paz Prieto, Paduraru, Alina, Ducker, Catherine, Marzaganov, Erik, Laviron, Marie, Kitano, Satsuki, Miyachi, Hitoshi, Crompton, Tessa, Ono, Masahiro |
| المصدر: | JOURNAL OF CELL BIOLOGY ; ISSN: 0021-9525 ; ISSN: 1540-8140 |
| سنة النشر: | 2018 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Biology and Life Sciences, Medicine and Health Sciences, REGULATORY T-CELLS, NEGATIVE SELECTION, FLUORESCENT TIMER, DNA METHYLATION, FOXP3 LOCUS, THYMUS, PROTEIN, ACTIVATION, MIGRATION, PERSPECTIVE |
| الوصف: | Understanding the mechanisms of cellular differentiation is challenging because differentiation is initiated by signaling pathways that drive temporally dynamic processes, which are difficult to analyze in vivo. We establish a new tool, Timer of cell kinetics and activity (Tocky; or toki [time in Japanese]). Tocky uses the fluorescent Timer protein, which spontaneously shifts its emission spectrum from blue to red, in combination with computer algorithms to reveal the dynamics of differentiation in vivo. Using a transcriptional target of T cell receptor (TCR) signaling, we establish Nr4a3-Tocky to follow downstream effects of TCR signaling. Nr4a3-Tocky reveals the temporal sequence of events during regulatory T cell (Treg) differentiation and shows that persistent TCR signals occur during Treg generation. Remarkably, antigen-specific T cells at the site of autoimmune inflammation also show persistent TCR signaling. In addition, by generating Foxp3-Tocky, we reveal the in vivo dynamics of demethylation of the Foxp3 gene. Thus, Tocky is a tool for cell biologists to address previously inaccessible questions by directly revealing dynamic processes in vivo. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://biblio.ugent.be/publication/01GJYQ2JZMG20C726V2PY129NSTest; http://hdl.handle.net/1854/LU-01GJYQ2JZMG20C726V2PY129NSTest; http://dx.doi.org/10.1083/jcb.201711048Test; https://biblio.ugent.be/publication/01GJYQ2JZMG20C726V2PY129NS/file/01GJYQ83J9YTHHWVHHHM59THEVTest |
| DOI: | 10.1083/jcb.201711048 |
| الإتاحة: | https://doi.org/10.1083/jcb.201711048Test https://biblio.ugent.be/publication/01GJYQ2JZMG20C726V2PY129NSTest http://hdl.handle.net/1854/LU-01GJYQ2JZMG20C726V2PY129NSTest https://biblio.ugent.be/publication/01GJYQ2JZMG20C726V2PY129NS/file/01GJYQ83J9YTHHWVHHHM59THEVTest |
| حقوق: | Creative Commons Attribution 4.0 International Public License (CC-BY 4.0) ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.59BB6BF6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1083/jcb.201711048 |
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