دورية أكاديمية
Development of the nanobody display technology to target lentiviral vectors to antigen-presenting cells
| العنوان: | Development of the nanobody display technology to target lentiviral vectors to antigen-presenting cells |
|---|---|
| المؤلفون: | Goyvaerts, Cleo, De Groeve, Kurt, Dingemans, Jozef, Van Lint, Sandra, Robays, Lander, Heirman, Carlo, Reiser, J, Thielemans, Kris, De Baetselier, Patrick, Raes, Geert, Breckpot, Karine |
| المصدر: | GENE THERAPY ; ISSN: 0969-7128 |
| سنة النشر: | 2012 |
| المجموعة: | Ghent University Academic Bibliography |
| مصطلحات موضوعية: | Medicine and Health Sciences, CANCER-IMMUNOTHERAPY, GENETIC-MODIFICATION, IN-VIVO, DENDRITIC CELLS, VESICULAR STOMATITIS-VIRUS, targeting, lentiviral vector, antigen-presenting cell, Nanobody, T-CELLS, TRANSDUCTION, IMMUNIZATION, IMMUNITY, THERAPY |
| الوصف: | Lentiviral vectors (LVs) provide unique opportunities for the development of immunotherapeutic strategies, as they transduce a variety of cells in situ, including antigen-presenting cells (APCs). Engineering LVs to specifically transduce APCs is required to promote their translation towards the clinic. We report on the Nanobody (Nb) display technology to target LVs to dendritic cells (DCs) and macrophages. This innovative approach exploits the budding mechanism of LVs to incorporate an APC-specific Nb and a binding-defective, fusion-competent form of VSV. G in the viral envelope. In addition to production of high titer LVs, we demonstrated selective, Nb-dependent transduction of mouse DCs and macrophages both in vitro and in situ. Moreover, this strategy was translated to a human model in which selective transduction of in vitro generated or lymph node (LN)-derived DCs and macrophages, was demonstrated. In conclusion, the Nb display technology is an attractive approach to generate LVs targeted to specific cell types. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://biblio.ugent.be/publication/6962893Test; http://hdl.handle.net/1854/LU-6962893Test; http://dx.doi.org/10.1038/gt.2011.206Test; https://biblio.ugent.be/publication/6962893/file/6963435Test |
| DOI: | 10.1038/gt.2011.206 |
| الإتاحة: | https://doi.org/10.1038/gt.2011.206Test https://biblio.ugent.be/publication/6962893Test http://hdl.handle.net/1854/LU-6962893Test https://biblio.ugent.be/publication/6962893/file/6963435Test |
| حقوق: | No license (in copyright) ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.7728B2B2 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1038/gt.2011.206 |
|---|