دورية أكاديمية
Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts
| العنوان: | Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts |
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| المؤلفون: | Zhang, Chi, Jia, Pingping, Huang, Xupei, Sferrazza, Gian Franco, Athauda, Gagani, Achary, Mohan P., Wang, Jikui, Lemanski, Sharon L., Lemanski, Larry F. |
| المجموعة: | FAU Digital Collections (Florida Atlantic University Digital Library) |
| مصطلحات موضوعية: | Ambystoma mexicanum --embryology, Ambystoma mexicanum --genetics, Base Sequence, DNA, Complementary --genetics, Endoderm --physiology, Exons --genetics, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Genes, Recessive, Heart --embryology, Models, Genetic, Molecular Sequence Data, Muscle Proteins --biosynthesis, Muscle Proteins --genetics, Myocardial Contraction --physiology, Protein Isoforms, Reverse Transcriptase Polymerase Chain Reaction, Tropomyosin |
| الوصف: | The Mexican axolotl, Ambystoma mexicanum, carries the naturally-occurring recessive mutant gene'c' that results in a failure of homozygous (c/c) embryos to form hearts that beat because of an absence of organized myofibrils. Our previous studies have shown that a noncoding RNA, Myofibril-Inducing RNA (MIR), is capable of promoting myofibrillogenesis and heart beating in the mutant (c/c) axolotls. The present study demonstrates that the MIR gene is essential for tropomyosin (TM) expression in axolotl hearts during development. Gene expression studies show that mRNA expression of various tropomyosin isoforms in untreated mutant hearts and in normal hearts knocked down with double-stranded MIR (dsMIR) are similar to untreated normal. However, at the protein level, selected tropomyosin isoforms are significantly reduced in mutantand dsMIR treated normal hearts. These results suggest that MIR is involved in controlling the translation or post-translation of various TM isoforms and subsequently of regulating cardiac contractility. ; Department of Biomedical Sciences, Florida Atlantic University |
| نوع الوثيقة: | text |
| اللغة: | English |
| DOI: | 10.1186/1423-0127-16-81 |
| الإتاحة: | https://doi.org/10.1186/1423-0127-16-81Test http://purl.flvc.org/fcla/dt/3327272Test https://fau.digital.flvc.org/islandora/object/fau%3A1899/datastream/TN/view/Myofibril-Inducing%20RNA%20%28MIR%29%20is%20essential%20for%20tropomyosin%20expression%20and%20myofibrillogenesis%20in%20axolotl%20hearts.jpgTest |
| رقم الانضمام: | edsbas.CCB609C |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/1423-0127-16-81 |
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