دورية أكاديمية
Interplay between the catabolite repression control protein Crc, Hfq and RNA in Hfq-dependent translational regulation in Pseudomonas aeruginosa
| العنوان: | Interplay between the catabolite repression control protein Crc, Hfq and RNA in Hfq-dependent translational regulation in Pseudomonas aeruginosa |
|---|---|
| المؤلفون: | Sonnleitner, Elisabeth, Wulf, Alexander, Campagne, Sébastien, Pei, Xue-Yuan, Wolfinger, Michael T., Forlani, Giada, Prindl, Konstantin, Abdou, Laetitia, Resch, Armin, Allain, Frédéric H.-T., Luisi, Ben F., Urlaub, Henning, Bläsi, Udo |
| المصدر: | Nucleic Acids Research, 46 (3) |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2018 |
| المجموعة: | ETH Zürich Research Collection |
| الوصف: | In Pseudomonas aeruginosa the RNA chaperone Hfq and the catabolite repression control protein (Crc) act as post-transcriptional regulators during carbon catabolite repression (CCR). In this regard Crc is required for full-fledged Hfq-mediated translational repression of catabolic genes. RNAseq based transcriptome analyses revealed a significant overlap between the Crc and Hfq regulons, which in conjunction with genetic data supported a concerted action of both proteins. Biochemical and biophysical approaches further suggest that Crc and Hfq form an assembly in the presence of RNAs containing A-rich motifs, and that Crc interacts with both, Hfq and RNA. Through these interactions, Crc enhances the stability of Hfq/Crc/RNA complexes, which can explain its facilitating role in Hfq-mediated translational repression. Hence, these studies revealed for the first time insights into how an interacting protein can modulate Hfq function. Moreover, Crc is shown to interfere with binding of a regulatory RNA to Hfq, which bears implications for riboregulation. These results are discussed in terms of a working model, wherein Crc prioritizes the function of Hfq toward utilization of favored carbon sources. ; ISSN:1362-4962 ; ISSN:0301-5610 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/000425294400041; http://hdl.handle.net/20.500.11850/247419Test |
| DOI: | 10.3929/ethz-b-000247419 |
| الإتاحة: | https://doi.org/20.500.11850/247419Test https://doi.org/10.3929/ethz-b-000247419Test https://doi.org/10.1093/nar/gkx1245Test https://hdl.handle.net/20.500.11850/247419Test |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0Test/ ; Creative Commons Attribution 4.0 International |
| رقم الانضمام: | edsbas.D97AC93B |
| قاعدة البيانات: | BASE |
| DOI: | 10.3929/ethz-b-000247419 |
|---|