دورية أكاديمية
Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution
| العنوان: | Cell Types of the Human Retina and Its Organoids at Single-Cell Resolution |
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| المؤلفون: | Cowan, Cameron S., Renner, Magdalena, De Gennaro, Martina, Gross Scherf, Brigitte, Goldblum, David, Hou, Yanyan, Munz, Martin, Rodrigues, Tiago M., Krol, Jacek, Szikra, Tamas, Cuttat, Rachel, Waldt, Annick, Papasaikas, Panagiotis, Diggelmann, Roland, Patino Alvarez, Claudia P., Galliker, Patricia, Spirig, Stefan E., Pavlinic, Dinko, Gerber-Hollbach, Nadine, Schuierer, Sven, Srdanovic, Aldin, Balogh, Marton, Panero, Riccardo, Kusnyerik, Ákos, Szabo, Arnold, Stadler, Michael B., Orgül, Selim, Picelli, Simone, Hasler, Pascal W., Hierlemann, Andreas, id_orcid:0 000-0002-3838-2468, Scholl, Hendrik P.N., Roma, Guglielmo, Nigsch, Florian, Roska, Botond |
| المصدر: | Cell, 182 (6) |
| بيانات النشر: | Cell Press |
| سنة النشر: | 2020 |
| المجموعة: | ETH Zürich Research Collection |
| الوصف: | Human organoids recapitulating the cell-type diversity and function of their target organ are valuable for basic and translational research. We developed light-sensitive human retinal organoids with multiple nuclear and synaptic layers and functional synapses. We sequenced the RNA of 285,441 single cells from these organoids at seven developmental time points and from the periphery, fovea, pigment epithelium and choroid of light-responsive adult human retinas, and performed histochemistry. Cell types in organoids matured in vitro to a stable “developed” state at a rate similar to human retina development in vivo. Transcriptomes of organoid cell types converged toward the transcriptomes of adult peripheral retinal cell types. Expression of disease-associated genes was cell-type-specific in adult retina, and cell-type specificity was retained in organoids. We implicate unexpected cell types in diseases such as macular degeneration. This resource identifies cellular targets for studying disease mechanisms in organoids and for targeted repair in human retinas. ; ISSN:0092-8674 ; ISSN:1097-4172 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/000571443300022; info:eu-repo/grantAgreement/SNF/Sinergia/141801; http://hdl.handle.net/20.500.11850/441335Test |
| DOI: | 10.3929/ethz-b-000441335 |
| الإتاحة: | https://doi.org/20.500.11850/441335Test https://doi.org/10.3929/ethz-b-000441335Test https://doi.org/10.1016/j.cell.2020.08.013Test https://hdl.handle.net/20.500.11850/441335Test |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by/4.0Test/ ; Creative Commons Attribution 4.0 International |
| رقم الانضمام: | edsbas.14874DD6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3929/ethz-b-000441335 |
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