دورية أكاديمية
A rapid method for assessing the RNA-binding potential of a protein
| العنوان: | A rapid method for assessing the RNA-binding potential of a protein |
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| المؤلفون: | Bendak, Katerina, Loughlin, Fionna E., Cheung, V., O'Connell, Mitchell R., Crossley, Merlin, Mackay, Joel P. |
| المصدر: | Nucleic Acids Research, 40 (14) |
| بيانات النشر: | Oxford University Press |
| سنة النشر: | 2012 |
| المجموعة: | ETH Zürich Research Collection |
| مصطلحات موضوعية: | Protein-nucleic acid interaction |
| الوصف: | In recent years, evidence has emerged for the existence of many diverse types of RNA, which play roles in a wide range of biological processes in all kingdoms of life. These molecules generally do not, however, act in isolation, and identifying which proteins partner with RNA is a major challenge. Many methods, in vivo and in vitro , have been used to address this question, including combinatorial or high-throughput approaches, such as systematic evolution of ligands, cross-linking and immunoprecipitation and RNA immunoprecipitation combined with deep sequencing. However, most of these methods are not trivial to pursue and often require substantial optimization before results can be achieved. Here, we demonstrate a simple technique that allows one to screen proteins for RNA-binding properties in a gel-shift experiment and can be easily implemented in any laboratory. This assay should be a useful first-pass tool for assessing whether a protein has RNA- or DNA-binding properties, prior to committing resources to more complex procedures. ; ISSN:1362-4962 ; ISSN:0301-5610 |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/application/pdf |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/wos/000307504700002; http://hdl.handle.net/20.500.11850/54619Test |
| DOI: | 10.3929/ethz-b-000054619 |
| الإتاحة: | https://doi.org/20.500.11850/54619Test https://doi.org/10.3929/ethz-b-000054619Test https://doi.org/10.1093/nar/gks285Test https://hdl.handle.net/20.500.11850/54619Test |
| حقوق: | info:eu-repo/semantics/openAccess ; http://creativecommons.org/licenses/by-nc/3.0Test ; Creative Commons Attribution-NonCommercial 3.0 Unported |
| رقم الانضمام: | edsbas.63B75DA6 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3929/ethz-b-000054619 |
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