Mutational bias in spermatogonia impacts the anatomy of regulatory sites in the human genome ; Human ATAC-seq narrow peaks from FGFR3 sorted adult spermatogonial cells
| العنوان: | Mutational bias in spermatogonia impacts the anatomy of regulatory sites in the human genome ; Human ATAC-seq narrow peaks from FGFR3 sorted adult spermatogonial cells |
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| المؤلفون: | Talmane, Lana, Kaiser, Vera, Kumar, Yatendra, Semple, Fiona, MacLennan, Marie, Semple, Colin, Fitzpatrick, David, Taylor, Martin |
| المساهمون: | MRC - Medical Research Council, Taylor, Martin |
| بيانات النشر: | University of Edinburgh. MRC IGC. MRC Human Genetics Unit |
| سنة النشر: | 2021 |
| المجموعة: | Edinburgh DataShare (University of Edinburgh) |
| مصطلحات موضوعية: | ATAC-seq, Mutation, Human, Spermatogonial, Germline, Breakpoint, Insertion, FGFR3, Genome, Biological Sciences |
| الوصف: | ATAC-Seq reports local chromatin accessibility and provides a snapshot of active regulatory regions and genomic regions occupied by DNA-binding proteins in a given tissue. We used ATAC-Seq to identify open chromatin sites in FGFR3-positive spermatogonial cells isolated from dissociated human testicular samples. FGFR3 is most highly expressed in self-renewing spermatogonial stem cells, with low expression also being detected in early differentiating spermatogonia; its expression thus overlaps with the onset of PRDM9 expression in pre-meiotic spermatogonia. Open chromatin in FGFR3-positive cells was identified using standard peak detection analysis with MACS2 software. ; See README.txt for data processing and format details. * Replicate_1.narrowPeak.bed - replicate 1 data containing merged reads of patient H.5 libraries 1 and 4 (FGFR3 positive large high side-scatter spermatogonial cells). * Replicate_2.narrowPeak.bed - replicate 2 data containing merged reads of patient H.5 libraries 2 and 5 (FGFR3 positive large spermatogonial cells). * Replicate_3.narrowPeak.bed - replicate 3 data containing merged reads of patient H.7 and H.10 (FGFR3 positive large spermatogonial cells). |
| نوع الوثيقة: | dataset |
| وصف الملف: | text/plain; application/octet-stream |
| اللغة: | English |
| العلاقة: | https://doi.org/10.1101/2021.06.10.447556Test; Talmane, Lana; Kaiser, Vera; Kumar, Yatendra; Semple, Fiona; MacLennan, Marie; Semple, Colin; Fitzpatrick, David; Taylor, Martin. (2021). Mutational bias in spermatogonia impacts the anatomy of regulatory sites in the human genome, [dataset]. University of Edinburgh. MRC IGC. MRC Human Genetics Unit. https://doi.org/10.7488/ds/3053Test.; https://hdl.handle.net/10283/3922Test; https://doi.org/10.7488/ds/3053Test |
| DOI: | 10.7488/ds/3053 |
| الإتاحة: | https://doi.org/10.7488/ds/3053Test https://doi.org/10.1101/2021.06.10.447556Test https://hdl.handle.net/10283/3922Test |
| حقوق: | Creative Commons Attribution 4.0 International Public License |
| رقم الانضمام: | edsbas.2EC0A31A |
| قاعدة البيانات: | BASE |
| DOI: | 10.7488/ds/3053 |
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