رسالة جامعية
Habenula-hypothalamic neural circuit as a target for compulsive eating behaviour ; Le circuit neuronal habenula-hypothalamique en tant que cible du comportement alimentaire compulsif
| العنوان: | Habenula-hypothalamic neural circuit as a target for compulsive eating behaviour ; Le circuit neuronal habenula-hypothalamique en tant que cible du comportement alimentaire compulsif |
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| المؤلفون: | Didelot, Paula |
| المساهمون: | Institut Agro Rennes Angers, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), INSERM, Centre de recherche en biomédecine de Strasbourg (CRBS), 1 rue Eugène Boeckel, 67000 Strasbourg, Frédéric Lecerf, Hasan Mohammad |
| المصدر: | https://dumas.ccsd.cnrs.fr/dumas-04338854Test ; Life Sciences [q-bio]. 2023. |
| بيانات النشر: | HAL CCSD |
| سنة النشر: | 2023 |
| المجموعة: | DUMAS (Dépôt Universitaire de Mémoires Après Soutenance) |
| مصطلحات موضوعية: | Obesity, Compulsive eating behaviour, Habenula, Hypothalamus, Zona incerta, Opioid, MOR-Cre, GABA neurons, Obésité, Comportement compulsif alimentaire, Opioïde, Neurone GABAergique, [SDV]Life Sciences [q-bio] |
| الوصف: | Obesity affects 650 million people worldwide and causes 2.8 million deaths every year. It is a disease that is on the increase, not least because of our society of over-consumption. Among this population, a significant proportion of these people show signs of compulsive eating. It is against this backdrop that research aimed at gaining a better understanding of the neural mechanisms involved in limiting these compulsive behaviours has emerged. This project focuses primarily on the habenula-hypothalamus circuit. The habenula has neuronal projections to the hypothalamus and also strongly expresses opioid receptors, which are involved in compulsive eating behaviour. The hypothesis of this project is therefore to test whether the habenula-hypothalamus circuit can be targeted to reduce compulsive eating behaviour. The objectives are these ones: 1) Establishing a paradigm of compulsive eating behaviour, 2) Characterising the habenula-hypothalamus circuit by mapping, 3) Targetting this circuit to reduce compulsive eating behaviour. To test this hypothesis, an initial experiment was carried out on VGAT:Ai14 mice to subject them to a compulsive eating behaviour paradigm. Active GABAergic neurons (inhibitory neurons) were quantified in the Zona Incerta. The second experiment uses MOR-Cre mice in which the synaptophysin virus is injected to mark synaptic endings and precisely to determine the regions of neuronal projections. The results were obtained using confocal microscopy with ZEN and ImageJ software. The results show an increase in the activation of GABAergic neurons in the Zona Incerta during a diet rich in fats and sugars. Neuronal projections and more precisely synaptic endings are observed in the hypothalamic region, and in particular in the Zona Incerta, the tuberous nuclei, the lateral hypothalamic area and the dorsomedian nuclei of the hypothalamus. These results show that there is an increase in the activation of GABAergic neurons in the context of compulsive eating behaviour. They also show that there appears ... |
| نوع الوثيقة: | master thesis |
| اللغة: | English |
| العلاقة: | dumas-04338854; https://dumas.ccsd.cnrs.fr/dumas-04338854Test; https://dumas.ccsd.cnrs.fr/dumas-04338854/documentTest; https://dumas.ccsd.cnrs.fr/dumas-04338854/file/2023_Didelot_Paula_BMC.pdfTest |
| الإتاحة: | https://dumas.ccsd.cnrs.fr/dumas-04338854Test https://dumas.ccsd.cnrs.fr/dumas-04338854/documentTest https://dumas.ccsd.cnrs.fr/dumas-04338854/file/2023_Didelot_Paula_BMC.pdfTest |
| رقم الانضمام: | edsbas.F507B1FC |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |