دورية أكاديمية
High-intensity interval training in hypoxia does not affect muscle HIF responses to acute hypoxia in humans
| العنوان: | High-intensity interval training in hypoxia does not affect muscle HIF responses to acute hypoxia in humans |
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| المؤلفون: | De Smet, S, D'Hulst, G, Poffe, C, Van Thienen, R, BERARDI, Emanuele, Hespel, P |
| المساهمون: | orcid:0000-0002-0775-9605 |
| بيانات النشر: | SPRINGER |
| سنة النشر: | 2021 |
| المجموعة: | Document Server@UHasselt (Universiteit Hasselt) |
| مصطلحات موضوعية: | Altitude training, High-intensity interval training, Human skeletal muscle, Hypoxia-inducible factor, Ischaemia, Near-infrared spectroscopy, Adult, Altitude, Humans, Hypoxia, Hypoxia-Inducible Factor 1, Male, Muscle, Skeletal, Oxygen Consumption, RNA, Messenger |
| الوصف: | The myocellular response to hypoxia is primarily regulated by hypoxia-inducible factors (HIFs). HIFs thus conceivably are implicated in muscular adaptation to altitude training. Therefore, we investigated the effect of hypoxic versus normoxic training during a period of prolonged hypoxia ('living high') on muscle HIF activation during acute ischaemia.Ten young male volunteers lived in normobaric hypoxia for 5 weeks (5 days per week, ~ 15.5 h per day, FiO2: 16.4-14.0%). One leg was trained in hypoxia (TRHYP, 12.3% FiO2) whilst the other leg was trained in normoxia (TRNOR, 20.9% FiO2). Training sessions (3 per week) consisted of intermittent unilateral knee extensions at 20-25% of the 1-repetition maximum. Before and after the intervention, a 10-min arterial occlusion and reperfusion of the leg was performed. Muscle oxygenation status was continuously measured by near-infrared spectroscopy. Biopsies were taken from m. vastus lateralis before and at the end of the occlusion.Irrespective of training, occlusion elevated the fraction of HIF-1α expressing myonuclei from ~ 54 to ~ 64% (P < 0.05). However, neither muscle HIF-1α or HIF-2α protein abundance, nor the expression of HIF-1α or downstream targets selected increased in any experimental condition. Training in both TRNOR and TRHYP raised muscular oxygen extraction rate upon occlusion by ~ 30%, whilst muscle hyperperfusion immediately following the occlusion increased by ~ 25% in either group (P < 0.05).Ten minutes of arterial occlusion increased HIF-1α-expressing myonuclei. However, neither normoxic nor hypoxic training during 'living high' altered muscle HIF translocation, stabilisation, or transcription in response to acute hypoxia induced by arterial occlusion. ; The authors thank all volunteers for their enthusiastic participation in this study. We also thank Monique Ramaekers for skillful assistance during the experiments. This work was supported by the Flemish Ministry of Sport, BLOSO—“Leerstoel Topsport Inspanningsfysiologie”. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | EUROPEAN JOURNAL OF APPLIED PHYSIOLOGY, 118 (4) , p. 847 -862; http://hdl.handle.net/1942/33199Test; 862; 847; 118; WOS:000427061700015 |
| DOI: | 10.1007/s00421-018-3820-4 |
| الإتاحة: | https://doi.org/10.1007/s00421-018-3820-4Test http://hdl.handle.net/1942/33199Test |
| حقوق: | Springer-Verlag GmbH Germany, part of Springer Nature 2018 ; info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.FD3D762D |
| قاعدة البيانات: | BASE |
| DOI: | 10.1007/s00421-018-3820-4 |
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