كتاب
High-Throughput MALDI-TOF Mass Spectrometry-Based Deubiquitylating Enzyme Assay for Drug Discovery
| العنوان: | High-Throughput MALDI-TOF Mass Spectrometry-Based Deubiquitylating Enzyme Assay for Drug Discovery |
|---|---|
| المؤلفون: | De Cesare, Virginia, Davies, Paul |
| المساهمون: | Maupin-Furlow, Julie, Edelmann, Mariola J. |
| المصدر: | De Cesare , V & Davies , P 2023 , High-Throughput MALDI-TOF Mass Spectrometry-Based Deubiquitylating Enzyme Assay for Drug Discovery . in J Maupin-Furlow & M J Edelmann (eds) , Deubiquitinases : Methods and Protocols . 1 edn , Methods in Molecular Biology , vol. 2591 , Humana Press , New York , pp. 123-134 . https://doi.org/10.1007/978-1-0716-2803-4_8Test |
| بيانات النشر: | Humana Press |
| سنة النشر: | 2023 |
| المجموعة: | Discovery - University of Dundee Online Publications |
| مصطلحات موضوعية: | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods, High-Throughput Screening Assays/methods, Drug Discovery/methods, Ubiquitin/metabolism, Enzyme Assays, Ubiquitin, DUBs, Deubiquitylating enzyme, High-throughput screening, MALDI-TOF mass spectrometry, Drug discovery, /dk/atira/pure/subjectarea/asjc/1300/1311, name=Genetics, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology |
| الوصف: | Ubiquitylation (or ubiquitination) is the reversible conjugation of a 76-amino-acid polypeptide (ubiquitin) to a target protein to modulate various biological processes. Deubiquitylating enzymes (DUBs) are a class of enzymes that specifically remove ubiquitin from a substrate. In recent years DUBs have garnered significant attention as a new class of targets in multiple therapeutic areas. The recent development of high-throughput Matrix-Assisted Laser Desorption/Ionization-Time of Flight mass spectrometry (MALDI-TOF MS) has provided new tools to perform drug discovery screening. Here we present a facile and high-throughput step-by-step protocol of the MALDI-TOF MS-based DUB assay for screening the activity of DUBs in vitro. In a MALDI-TOF DUB assay, we quantitate the amount of mono-ubiquitin generated by the in vitro cleavage of ubiquitin chains. The presented protocol takes advantage of nanoliter dispensing robotics and automated MALDI-TOF MS analysis to screen large and diverse compound libraries. |
| نوع الوثيقة: | book part |
| وصف الملف: | application/pdf |
| اللغة: | English |
| ردمك: | 978-1-07-162802-7 1-07-162802-X |
| العلاقة: | https://discovery.dundee.ac.uk/en/publications/9d9d5536-25f8-40a5-a142-70838c2895f1Test; urn:ISBN:9781071628027 |
| DOI: | 10.1007/978-1-0716-2803-4_8 |
| الإتاحة: | https://doi.org/10.1007/978-1-0716-2803-4_8Test https://discovery.dundee.ac.uk/en/publications/9d9d5536-25f8-40a5-a142-70838c2895f1Test https://discovery.dundee.ac.uk/ws/files/91097355/DeCesare_Davies_Final.pdfTest http://www.scopus.com/inward/record.url?scp=85141535671&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.890910 |
| قاعدة البيانات: | BASE |
| ردمك: | 9781071628027 107162802X |
|---|---|
| DOI: | 10.1007/978-1-0716-2803-4_8 |