Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9

التفاصيل البيبلوغرافية
العنوان:	Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9
المؤلفون:	Sateriale, Adam, Pawlowic, Mattie, Vinayak, Sumiti, Brooks, Carrie, Striepen, Boris
المساهمون:	Mead, Jan R., Arrowood, Michael J.
المصدر:	Sateriale , A , Pawlowic , M , Vinayak , S , Brooks , C & Striepen , B 2020 , Genetic Manipulation of Cryptosporidium parvum with CRISPR/Cas9 . in J R Mead & M J Arrowood (eds) , Cryptosporidium . Methods in Molecular Biology , vol. 2052 , Humana Press , New York , pp. 219-228 . https://doi.org/10.1007/978-1-4939-9748-0_13Test
بيانات النشر:	Humana Press
سنة النشر:	2020
المجموعة:	Discovery - University of Dundee Online Publications
مصطلحات موضوعية:	CRISPR, Cas9, Genetics, Transfection, Transgenics, /dk/atira/pure/subjectarea/asjc/1300/1312, name=Molecular Biology, /dk/atira/pure/subjectarea/asjc/1300/1311, name=Genetics
الوصف:	Cryptosporidium parvum can be reliably genetically manipulated using CRISPR/Cas9-driven homologous repair coupled to in vivo propagation within immunodeficient mice. Recent modifications have simplified the initial protocol significantly. This chapter will guide through procedures for excystation, transfection, infection, collection, and purification of transgenic Cryptosporidium parvum.
نوع الوثيقة:	book part
اللغة:	English
ردمك:	978-1-4939-9747-3 1-4939-9747-5
العلاقة:	https://discovery.dundee.ac.uk/en/publications/c077f70f-c44d-49b7-9a6e-e9b5a7650349Test; urn:ISBN:9781493997473
DOI:	10.1007/978-1-4939-9748-0_13
الإتاحة:	https://doi.org/10.1007/978-1-4939-9748-0_13Test https://discovery.dundee.ac.uk/en/publications/c077f70f-c44d-49b7-9a6e-e9b5a7650349Test http://www.scopus.com/inward/record.url?scp=85071634091&partnerID=8YFLogxKTest
حقوق:	info:eu-repo/semantics/restrictedAccess
رقم الانضمام:	edsbas.73226B3B
قاعدة البيانات:	BASE

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الوصف
ردمك:	9781493997473 1493997475
DOI:	10.1007/978-1-4939-9748-0_13