دورية أكاديمية
IL-27 induces an IFN-like signature in murine macrophages which in turn modulate colonic epithelium
| العنوان: | IL-27 induces an IFN-like signature in murine macrophages which in turn modulate colonic epithelium |
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| المؤلفون: | Andrews, Caroline, McLean, Mairi H., Hixon, Julie A., Pontejo, Sergio M., Starr, Tregei, Malo, Courtney, Cam, Margaret, Ridnour, Lisa, Hickman, Heather, Steele-Mortimer, Olivia, Wink, David A., Young, Howard A., McVicar, Daniel W., Li, Wenqing, Durum, Scott K. |
| المصدر: | Andrews , C , McLean , M H , Hixon , J A , Pontejo , S M , Starr , T , Malo , C , Cam , M , Ridnour , L , Hickman , H , Steele-Mortimer , O , Wink , D A , Young , H A , McVicar , D W , Li , W & Durum , S K 2023 , ' IL-27 induces an IFN-like signature in murine macrophages which in turn modulate colonic epithelium ' , Frontiers in Immunology , vol. 14 , 1021824 . https://doi.org/10.3389/fimmu.2023.1021824Test |
| سنة النشر: | 2023 |
| المجموعة: | Discovery - University of Dundee Online Publications |
| مصطلحات موضوعية: | Mice, Animals, Interleukin-27/therapeutic use, Colon, Inflammatory Bowel Diseases/drug therapy, Macrophages, Epithelium, IL-27 cytokine, IFN signature, IBD - inflammatory bowel disease, colonoid, /dk/atira/pure/subjectarea/asjc/2700/2723, name=Immunology and Allergy, /dk/atira/pure/subjectarea/asjc/2400/2403, name=Immunology |
| الوصف: | Mucosal delivery of IL-27 has been shown to have a therapeutic benefit in murine models of inflammatory bowel disease (IBD). The IL-27 effect was associated with phosphorylated STAT1 (pSTAT1), a product of IL27 receptor signaling, in bowel tissue. To determine whether IL-27 acted directly on colonic epithelium, murine colonoids and primary intact colonic crypts were shown to be unresponsive to IL-27 in vitro and to lack detectable IL-27 receptors. On the other hand, macrophages, which are present in inflamed colon tissue, were responsive to IL-27 in vitro. IL-27 induced pSTAT1 in macrophages, the transcriptome indicated an IFN-like signature, and supernatants induced pSTAT1 in colonoids. IL-27 induced anti-viral activity in macrophages and MHC Class II induction. We conclude that the effects of mucosal delivery of IL-27 in murine IBD are in part based on the known effects of IL27 inducing immunosuppression of T cells mediated by IL-10. We also conclude that IL-27 has potent effects on macrophages in inflamed colon tissue, generating mediators that in turn act on colonic epithelium. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| العلاقة: | https://discovery.dundee.ac.uk/en/publications/e3d9d4ea-d505-421a-86d7-f3670ae4974bTest |
| DOI: | 10.3389/fimmu.2023.1021824 |
| الإتاحة: | https://doi.org/10.3389/fimmu.2023.1021824Test https://discovery.dundee.ac.uk/en/publications/e3d9d4ea-d505-421a-86d7-f3670ae4974bTest https://discovery.dundee.ac.uk/ws/files/102491597/fimmu_14_1021824.pdfTest http://www.scopus.com/inward/record.url?scp=85158001615&partnerID=8YFLogxKTest |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.95338EF |
| قاعدة البيانات: | BASE |
| DOI: | 10.3389/fimmu.2023.1021824 |
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