دورية أكاديمية
Rabbit neurospheres as a novel in vitro tool for studying neurodevelopmental effects induced by intrauterine growth restriction
| العنوان: | Rabbit neurospheres as a novel in vitro tool for studying neurodevelopmental effects induced by intrauterine growth restriction |
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| المؤلفون: | Barenys Espadaler, Marta, Illa Armengol, Míriam, Hofrichter, Maxi, Loreiro, Carla, Pla, Laura, Klose, Jördis, Kühne, Britta Anna, Gómez Catalán, Jesús, Braun, Jan Matthias, Crispi Brillas, Fàtima, Gratacós Solsona, Eduard, Fritsche, Ellen |
| المصدر: | Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica) |
| بيانات النشر: | John Wiley & Sons |
| سنة النشر: | 2020 |
| المجموعة: | Dipòsit Digital de la Universitat de Barcelona |
| مصطلحات موضوعية: | Neurobiologia del desenvolupament, Creixement fetal, Developmental neurobiology, Fetal growth |
| الوصف: | The aim of this study was to develop a rabbit neurosphere culture to characterize differences in basic processes of neurogenesis induced by intrauterine growth restriction (IUGR). A novel in vitro neurosphere culture has been established using fresh or frozen neural progenitor cells from newborn (PND0) rabbit brains. After surgical IUGR induction in pregnant rabbits and cesarean section 5 days later, neural progenitor cells from both control and IUGR groups were isolated and directly cultured or frozen at −80°C. These neural progenitor cells spontaneously formed neurospheres after 7 days in culture. The ability of control and IUGR neurospheres to migrate, proliferate, differentiate to neurons, astrocytes, or oligodendrocytes was compared and the possibility to modulate their responses was tested by exposure to several positive and negative controls. Neurospheres obtained from IUGR brains have a significant impairment in oligodendrocyte differentiation, whereas no significant differences are observed in other basic processes of neurogenesis. This impairment can be reverted by in vitro exposure of IUGR neurospheres to thyroid hormone, which is known to play an essential role in white matter maturation in vivo. Our new rabbit neurosphere model and the results of this study open the possibility to test several substances in vitro as neuroprotective candidates against IUGR induced neurodevelopmental damage while decreasing the number of animals and resources and allowing a more mechanistic approach at a cellular functional level. |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 13 p.; application/pdf |
| اللغة: | English |
| تدمد: | 2157-6564 33034168 |
| العلاقة: | Reproducció del document publicat a: https://doi.org/10.1002/sctm.20-0223Test; Stem Cells Translational Medicine, 2020, vol. 10, p. 209-221; https://doi.org/10.1002/sctm.20-0223Test; http://hdl.handle.net/2445/177846Test; 703776 |
| الإتاحة: | https://doi.org/10.1002/sctm.20-0223Test http://hdl.handle.net/2445/177846Test |
| حقوق: | cc-by (c) Barenys, Marta et al., 2020 ; https://creativecommons.org/licenses/by/4.0Test/ ; info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.52B801E7 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 21576564 33034168 |
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