دورية أكاديمية
Molecular epidemiology and virulence of Escherichia coli O16: H5-ST131: Comparison with H30 and H30-Rx subclones of O25b: H4-ST131
| العنوان: | Molecular epidemiology and virulence of Escherichia coli O16: H5-ST131: Comparison with H30 and H30-Rx subclones of O25b: H4-ST131 |
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| المؤلفون: | Dahbi, Ghizlane, Cruz, Fernando de la, Toro, María de, Blanco, Jorge |
| المساهمون: | Red Española de Investigación en Patología Infecciosa, Instituto de Salud Carlos III, Ministerio de Economía y Competitividad (España), European Commission, Xunta de Galicia, Junta de Andalucía, Ministerio de Asuntos Exteriores y Cooperación (España), Federación Española de Enfermedades Raras |
| بيانات النشر: | Elsevier |
| سنة النشر: | 2014 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | ST131 clonal group, O25b:H4, H30 and H30-Rx subclones, O16:H5, ExPEC, E. coli |
| الوصف: | et al. ; The present study was carried out to evaluate the prevalence of the clonal subgroup O16:H5-ST131 and the H30 and H30-Rx subclones among E. coli isolates causing extraintestinal infections and to know their virulence potential. The ST131 clonal group accounted for 490 (16%) of the 2995 isolates obtained from clinical samples in five Spanish hospitals during the study period (2005-2012). Among those 490 ST131 isolates, 456 belonged to serotype O25b:H4, 27 to O16:H5 and seven were O-non-typeable:H4 (ONT:H4). All 27 O16:H5 isolates showed fimH41, whereas fimH30 and fimH22 alleles were the most frequently detected among O25b:H4 isolates. The majority (381/490; 78%) of ST131 isolates belonged to H30 subclone, and 302 of 381 (79%) H30 isolates belonged to the H30-Rx subclone. Of the 27 O16:H5 isolates, 48% produced CTX-M-14; however, none produced CTX-M-15. In contrast, 46% of O25b:H4 isolates produced CTX-M-15 while only 2% produced CTX-M-14. More than a half of the O16:H5 isolates (56%) showed the ExPEC status which was significantly more prevalent within O25b:H4 isolates (81%) (P<. 0.01), especially among H30-Rx (97%) isolates. In the present study, a modified virotype scheme was applied within which approximately half (52%) of the O16:H5 isolates showed the C1 specific virotype. Despite their low virulence-gene score (mean of virulence genes 6.4 versus 8.5 in O25b:H4 isolates), six out of the 10 O16:H5 isolates assayed showed high virulence in the mouse model of sepsis (killed 90-100% of mice challenged). Furthermore, four O16:H5 isolates of virotypes A and C1, carrying K2 variant of group II capsule, showed lethality at 24. h. Thus, certain O16:H5 fimH41 isolates show a similar in vivo virulence to that reported with the highly virulent O25b:H4 H30-Rx isolates (Mora et al., PLOS ONE 2014, e87025), supporting their potential virulence for humans. ; This work was partially supported by the Red Española de Investigación en Patología Infecciosa (REIPI RD06/0008/1018-1016) and grants PI09/01273, PI10/01955 ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 1438-4221 |
| العلاقة: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/EC/FP7/289326; info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-47852R; info:eu-repo/grantAgreement/EC/FP7/282004; Sí; e-issn: 1618-0607; International Journal of Medical Microbiology 304(8): 1247-1257 (2014); http://hdl.handle.net/10261/130471Test; http://dx.doi.org/10.13039/501100004587Test; http://dx.doi.org/10.13039/501100003329Test; http://dx.doi.org/10.13039/501100000780Test; http://dx.doi.org/10.13039/501100003767Test; http://dx.doi.org/10.13039/501100002924Test; http://dx.doi.org/10.13039/501100011011Test; http://dx.doi.org/10.13039/501100010801Test |
| DOI: | 10.1016/j.ijmm.2014.10.002 |
| DOI: | 10.13039/501100004587 |
| DOI: | 10.13039/501100003329 |
| DOI: | 10.13039/501100000780 |
| DOI: | 10.13039/501100003767 |
| DOI: | 10.13039/501100002924 |
| DOI: | 10.13039/501100011011 |
| DOI: | 10.13039/501100010801 |
| الإتاحة: | https://doi.org/10.1016/j.ijmm.2014.10.002Test https://doi.org/10.13039/501100004587Test https://doi.org/10.13039/501100003329Test https://doi.org/10.13039/501100000780Test https://doi.org/10.13039/501100003767Test https://doi.org/10.13039/501100002924Test https://doi.org/10.13039/501100011011Test https://doi.org/10.13039/501100010801Test http://hdl.handle.net/10261/130471Test |
| حقوق: | none |
| رقم الانضمام: | edsbas.60CB1B26 |
| قاعدة البيانات: | BASE |
| تدمد: | 14384221 |
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| DOI: | 10.1016/j.ijmm.2014.10.002 |