دورية أكاديمية
RUST: A Robust, User-Friendly Script Tool for Rapid Measurement of Rust Disease on Cereal Leaves
| العنوان: | RUST: A Robust, User-Friendly Script Tool for Rapid Measurement of Rust Disease on Cereal Leaves |
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| المؤلفون: | Gallego-Sánchez, L., Canales, Francisco José, Montilla-Bascón, Gracia, Prats, Elena |
| المساهمون: | Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission |
| بيانات النشر: | Multidisciplinary Digital Publishing Institute |
| سنة النشر: | 2020 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Rust, Infection frequency, Disease severity, Image analysis, Fiji, ImageJ |
| الوصف: | © 2020 by the authors. ; Recently, phenotyping has become one of the main bottlenecks in plant breeding and fundamental plant science. This is particularly true for plant disease assessment, which has to deal with time-consuming evaluations and the subjectivity of visual assessments. In this work, we have developed an open source Robust, User-friendy Script Tool (RUST) for semi-automated evaluation of leaf rust diseases. RUST runs under the free Fiji imaging software (developed from ImageJ), which is a well-recognized software among the scientific community. The script enables the evaluation of leaf rust diseases using a color transformation tool and provides three different automation modes. The script opens images sequentially and records infection frequency (pustules per area) (semi-)automatically for high-throughput analysis. Furthermore, it can manage several scanned leaf segments in the same image, consecutively selecting the desired segments. The script has been validated with nearly 900 samples from 80 oat genotypes ranging from resistant to susceptible and from very light to heavily infected leaves showing a high accuracy with a Lin’s concordance correlation coefficient of 0.99. The analysis show a high repeatability as indicated by the low variation coefficients obtained when repeating the measurement of the same samples. The script also has optional steps for calibration and training to ensure accuracy, even in low-resolution images. This script can evaluate efficiently hundreds of leaves facilitating the screening of novel sources of resistance to this important cereal disease. ; This research was funded by Spanish Ministry of Science and Innovation [PID2019-104518RB-I00], (AEI/FEDER, UE), regional government through the AGR-253 group, and the European Regional and Social Development Funds. LG is holder of a FPI fellowship from the Spanish Ministry of Science and Innovation [BES-2017-080152]. ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| ردمك: | 978-2-01-708015-2 2-01-708015-2 |
| تدمد: | 2223-7747 |
| العلاقة: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-104518RB-I00; PID2019-104518RB-I00/AEI/10.13039/501100011033; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/BES-2017-080152; BES-2017-080152/AEI/10.13039/501100011033; Publisher’s version; https://doi.org/10.3390/plants9091182Test; Sí; Plants 9(9): 1182 (2020); http://hdl.handle.net/10261/220335Test; http://dx.doi.org/10.13039/501100000780Test; http://dx.doi.org/10.13039/501100011033Test |
| DOI: | 10.3390/plants9091182 |
| DOI: | 10.13039/501100000780 |
| DOI: | 10.13039/501100011033 |
| الإتاحة: | https://doi.org/10.3390/plants9091182Test https://doi.org/10.13039/501100000780Test https://doi.org/10.13039/501100011033Test http://hdl.handle.net/10261/220335Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.BD7157A0 |
| قاعدة البيانات: | BASE |
Full Text Finder | ردمك: | 9782017080152 2017080152 |
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| تدمد: | 22237747 |
| DOI: | 10.3390/plants9091182 |