دورية أكاديمية
Neuroprotection by glutamate oxaloacetate transaminase in ischemic stroke: An experimental study
| العنوان: | Neuroprotection by glutamate oxaloacetate transaminase in ischemic stroke: An experimental study |
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| المؤلفون: | Campos, Francisco, Sobrino, Tomás, Ramos-Cabrer, Pedro, Argibay, Bárbara, Agulla, Jesús, Pérez-Mato, María, Rodríguez-González, Raquel, Brea, David, Castillo, José |
| المساهمون: | Ministerio de Ciencia e Innovación (España), Instituto de Salud Carlos III, Xunta de Galicia, Fundación Mutua Madrileña |
| بيانات النشر: | Sage Publications |
| سنة النشر: | 2011 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Animal model of ischemia, Glutamate, Glutamate oxaloacetate transaminase, Magnetic resonance, Neuroprotection |
| الوصف: | As ischemic stroke is associated with an excessive release of glutamate into the neuronal extracellular space, a decrease in blood glutamate levels could provide a mechanism to remove it from the brain tissue, by increasing the brain-blood gradient. In this regard, the ability of glutamate oxaloacetate transaminase (GOT) to metabolize glutamate in blood could represent a potential neuroprotective tool for ischemic stroke. This study aimed to determine the neuroprotective effects of GOT in an animal model of cerebral ischemia by means of a middle cerebral arterial occlusion (MCAO) following the Stroke Therapy Academic Industry Roundtable (STAIR) group guidelines. In this animal model, oxaloacetate-mediated GOT activation inhibited the increase of blood and cerebral glutamate after MCAO. This effect is reflected in a reduction of infarct size, smaller edema volume, and lower sensorimotor deficits with respect to controls. Magnetic resonance spectroscopy confirmed that the increase of glutamate levels in the brain parenchyma after MCAO is inhibited after oxaloacetate-mediated GOT activation. These findings show the capacity of the GOT to remove glutamate from the brain by means of blood glutamate degradation, and suggest the applicability of this enzyme as an efficient and novel neuroprotective tool against ischemic stroke. ; This study was partially supported by grants from the Spanish Ministry of Science and Innovation SAF2008-00737 and Fondo de Investigaciones Sanitarias, Instituto Salud Carlos III, RETICS-RD06/0026 and PI081472; Xunta de Galicia (Consellería de Innovación, Industria e Comercio: 09CSA057918PR; Consellería de Sanidade: PS09/32 and Fundación Médica Mutua Madrileña. Furthermore, F Campos is recipient of fellowship from Conselleria deIndustria, Xunta de Galicia (Programa Angeles Alvariño) and P Ramos-Cabrer acknowledges the Instituto de Salud Carlos III for a research contract of the Miguel Servet program. ; Peer reviewed |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0271-678X 1559-7016 |
| العلاقة: | #PLACEHOLDER_PARENT_METADATA_VALUE#; info:eu-repo/grantAgreement/MICINN//SAF2008-00737/ES/APLICACION DE FACTORES DE CRECIMIENTO ESTIMULANTES DEL DESARROLLO Y DIFERENCIACION DE CELULAS PROGENITORAS ENDOTELIALES EN LA ISQUEMIA CEREBRAL: ESTUDIO CLINICO - EXPERIMENTAL/; https://doi.org/10.1038/jcbfm.2011.3Test; No; Journal of Cerebral Blood Flow and Metabolism 31(6): 1378-1386 (2011); http://hdl.handle.net/10261/343607Test |
| DOI: | 10.1038/jcbfm.2011.3 |
| الإتاحة: | https://doi.org/10.1038/jcbfm.2011.3Test http://hdl.handle.net/10261/343607Test |
| حقوق: | none |
| رقم الانضمام: | edsbas.28302E4F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 0271678X 15597016 |
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| DOI: | 10.1038/jcbfm.2011.3 |