دورية أكاديمية
Vitamin D receptor deficiency enhances Wnt/β-catenin signaling and tumor burden in colon cancer
| العنوان: | Vitamin D receptor deficiency enhances Wnt/β-catenin signaling and tumor burden in colon cancer |
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| المؤلفون: | Larriba, María Jesús, Ordóñez-Morán, Paloma, Muñoz Terol, Alberto, Pálmer, Héctor G. |
| بيانات النشر: | Public Library of Science |
| سنة النشر: | 2011 |
| المجموعة: | Digital.CSIC (Consejo Superior de Investigaciones Científicas / Spanish National Research Council) |
| مصطلحات موضوعية: | Beta-catenin, Stem-cells, Colorectal cancer, Adult epidermis, E-cadherin, Wnt |
| الوصف: | et al. ; Aberrant activation of the Wnt/beta-catenin pathway is critical for the initiation and progression of most colon cancers. This activation provokes the accumulation of nuclear beta-catenin and the induction of its target genes. Apc(min/+) mice are the most commonly used model for colon cancer. They harbor a mutated Apc allele and develop intestinal adenomas and carcinomas during the first months of life. This phenotype is caused by the mutation of the second Apc allele and the consequent accumulation of nuclear beta-catenin in the affected cells. Here we describe that vitamin D receptor (VDR) is a crucial modulator of nuclear beta-catenin levels in colon cancer in vivo. By appropriate breeding of Apc(min/+) mice and Vdr(+/-) mice we have generated animals expressing a mutated Apc allele and two, one, or none Vdr wild type alleles. Lack of Vdr increased the number of colonic Aberrant Crypt Foci (ACF) but not that of adenomas or carcinomas in either small intestine or colon. Importantly, colon ACF and tumors of Apc(min/+) Vdr(-/-) mice had increased nuclear beta-catenin and the tumors reached a larger size than those of Apc(min/+) Vdr(+/+). Both ACF and carcinomas in Apc(min/+) Vdr(-/-) mice showed higher expression of beta-catenin/TCF target genes. In line with this, VDR knock-down in cultured human colon cancer cells enhanced beta-catenin nuclear content and target gene expression. Consistently, VDR depletion abrogated the capacity of 1,25(OH)(2)D(3) to promote the relocation of beta-catenin from the nucleus to the plasma membrane and to inhibit beta-catenin/TCF target genes. In conclusion, VDR controls the level of nuclear beta-catenin in colon cancer cells and can therefore attenuate the impact of oncogenic mutations that activate the Wnt/beta-catenin pathway. ; This work was supported by Fondo de Investigación Sanitaria-Instituto de Salud Carlos III (ISCIII, PI081356), Vall d’Hebrón Institute of Oncology, Olga Torres Foundation, Fundación de la Asociación Española de Lucha Contra el Cáncer (AECC), ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1932-6203 |
| العلاقة: | Publisher’s version; http://dx.doi.org/10.1371/journal.pone.0023524Test; PLoS ONE 6(8): e23524 (2011); http://hdl.handle.net/10261/40079Test |
| DOI: | 10.1371/journal.pone.0023524 |
| الإتاحة: | https://doi.org/10.1371/journal.pone.0023524Test http://hdl.handle.net/10261/40079Test |
| حقوق: | open |
| رقم الانضمام: | edsbas.623FDDD2 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0023524 |