دورية أكاديمية
Nicotine Enhances Alcoholic Fatty Liver in Mice: Role of CYP2A5
| العنوان: | Nicotine Enhances Alcoholic Fatty Liver in Mice: Role of CYP2A5 |
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| المؤلفون: | Chen, Xue, Owoseni, Emmanuel, Salamat, Julia, Cederbaum, Arthur I., Lu, Yongke |
| المصدر: | ETSU Faculty Works |
| بيانات النشر: | Digital Commons @ East Tennessee State University |
| سنة النشر: | 2018 |
| المجموعة: | Digital Commons @ East Tennessee State University |
| مصطلحات موضوعية: | cotinine, CYP2A6, metabolism, oxidative stress, reactive oxygen species, triglyceride, Health Sciences |
| الوصف: | Tobacco and alcohol are often co-abused. Nicotine can enhance alcoholic fatty liver, and CYP2A6 (CYP2A5 in mice), a major metabolism enzyme for nicotine, can be induced by alcohol. CYP2A5 knockout (cyp2a5−/−) mice and their littermates (cyp2a5+/+) were used to test whether CYP2A5 has an effect on nicotine-enhanced alcoholic fatty liver. The results showed that alcoholic fatty liver was enhanced by nicotine in cyp2a5+/+ mice but not in the cyp2a5−/− mice. Combination of ethanol and nicotine increased serum triglyceride in cyp2a5+/+ mice but not in the cyp2a5−/− mice. Cotinine, a major metabolite of nicotine, also enhanced alcoholic fatty liver, which was also observed in cyp2a5+/+ mice but not in the cyp2a5−/− mice. Nitrotyrosine and malondialdehyde (MDA), markers of oxidative/nitrosative stress, were induced by alcohol and were further increased by nicotine and cotinine in cyp2a5+/+ mice but not in the cyp2a5−/− mice. Reactive oxygen species (ROS) production during microsomal metabolism of nicotine and cotinine was increased in microsomes from cyp2a5+/+ mice but not in microsomes from cyp2a5−/− mice. These results suggest that nicotine enhances alcoholic fatty liver in a CYP2A5-dependent manner, which is related to ROS produced during the process of CYP2A5-dependent nicotine metabolism. |
| نوع الوثيقة: | text |
| اللغة: | unknown |
| العلاقة: | https://dc.etsu.edu/etsu-works/10197Test; https://doi.org/10.1016/j.abb.2018.09.012Test |
| DOI: | 10.1016/j.abb.2018.09.012 |
| الإتاحة: | https://doi.org/10.1016/j.abb.2018.09.012Test https://dc.etsu.edu/etsu-works/10197Test |
| رقم الانضمام: | edsbas.A3408E7 |
| قاعدة البيانات: | BASE |
| DOI: | 10.1016/j.abb.2018.09.012 |
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