دورية أكاديمية
The Role of the Prodomain in the Folding of Carboxypeptidase A1
| العنوان: | The Role of the Prodomain in the Folding of Carboxypeptidase A1 |
|---|---|
| المؤلفون: | Domecillo, Masy |
| المصدر: | Honors Theses |
| بيانات النشر: | Digital Commons @ Andrews University |
| سنة النشر: | 2022 |
| المجموعة: | Digital Commons @ Andrews University |
| مصطلحات موضوعية: | Carbosypeptidase A1, Enzymes, Digestion, Prodomain, Carboxypeptidases, Digestive enzymes, Biology |
| الوصف: | Carboxypeptidase A1 (CPA1) is an enzyme utilized in digestion. CPA1 contains a prodomain region, which must be cleaved for enzymatic function. The prodomain region has been hypothesized to be necessary for structural stability, as previous studies were unable to produce a functional CPA1 protein without the prodomain region. However, the recent discovery of a related enzyme, Carboxypeptidase 0 (CP0), which does not have a prodomain yet functions enzymatically, challenges this assertion. To determine if the prodomain is essential to the folding of CPA1, an HA-tag was added for detection, and the prodomain was deleted via PCR. A Western blot was performed to determine expression and enzyme assays were conducted to determine function. CPA1 without a prodomain was detected on the Western blot, but did not display activity in the enzyme assays. This indicates that CPA1 without a prodomain folds adequately enough to be expressed but is nonfunctional. Therefore, the prodomain region appears to be necessary for CPA1 structure and functionality. |
| نوع الوثيقة: | text |
| وصف الملف: | application/pdf |
| اللغة: | unknown |
| العلاقة: | https://digitalcommons.andrews.edu/honors/262Test; https://digitalcommons.andrews.edu/context/honors/article/1264/viewcontent/Domecillo_Masy.pdfTest |
| الإتاحة: | https://digitalcommons.andrews.edu/honors/262Test https://digitalcommons.andrews.edu/context/honors/article/1264/viewcontent/Domecillo_Masy.pdfTest |
| حقوق: | http://creativecommons.org/licenses/by-nc-nd/4.0Test/ |
| رقم الانضمام: | edsbas.4649A50 |
| قاعدة البيانات: | BASE |
| الوصف غير متاح. |