التفاصيل البيبلوغرافية
| العنوان: |
Development of novel alphavirus expression and detection systems and characterization of oxidation's effect on viral replication |
| المؤلفون: |
Steel, J. Jordan, author, Geiss, Brian, advisor, Schenkel, Alan, committee member, Olson, Ken, committee member, Callan, Rob, committee member |
| بيانات النشر: |
Colorado State University. Libraries |
| سنة النشر: |
2007 |
| المجموعة: |
Digital Collections of Colorado (Colorado State University) |
| مصطلحات موضوعية: |
flavivirus, Sindbis, alphavirus, Aedes aegypti, RNA, Dengue |
| الوصف: |
2014 Spring. ; Includes bibliographical references. ; Infectious diseases cause significant global suffering and death each year. Specifically, arthropod-borne viruses are emerging and re-emerging around the world and infecting millions of people. Mosquitoes that transmit these viruses are spreading to new regions of the world with naïve populations to serve as viral hosts. Climate change and human encroachment of habitat has brought mosquitoes into close proximity with humans. The viruses are evolving and are expanding their vector compatibility to include more than one species of mosquitoes. The combination of these elements results in a serious global need to develop ways to control or prevent arthropod-borne viruses. In order to discover novel antivirals and ways to inhibit these arboviruses, a better understanding of viral infection and replication is needed. This dissertation will describe a combination of projects that all aim to provide enhanced knowledge or tools to prevent, control, or treat arbovirus infection. Specifically, we improved the ability to express recombinant infectious alphaviruses, developed a novel system to detect alphavirus infection in mosquito cell culture and transgenic mosquitoes, and discovered a new role for oxidation during flavivirus replication. First, we successfully developed and established a method for transcribing infectious alphavirus RNA from a plasmid DNA platform. This approach provides an efficient way for producing high titer infectious recombinant alphavirus in multiple cell types that robustly express foreign proteins. Secondly, we optimized a system for detecting alphavirus infection in mosquito cells using the virus dependent subgenomic promoter to transcribe a reporter gene only during active infection. We demonstrated that mosquito cells can be stably transformed to transcribe an engineered viral reporter RNA that expresses a fluorescent reporter protein (mCherry) only in the presence of wild-type virus infection. The reporter protein is not detected in ... |
| نوع الوثيقة: |
text |
| وصف الملف: |
born digital; doctoral dissertations; application/pdf |
| اللغة: |
English |
| العلاقة: |
Steel_colostate_0053A_12290.pdf; http://hdl.handle.net/10217/82658Test |
| الإتاحة: |
http://hdl.handle.net/10217/82658Test |
| حقوق: |
Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyrightTest. |
| رقم الانضمام: |
edsbas.86C0CD1D |
| قاعدة البيانات: |
BASE |
