التفاصيل البيبلوغرافية
| العنوان: |
Environmental programming of respiratory allergy: utility of a child’s spit epigenome |
| المؤلفون: |
Langie, Sabine A. S., Moisse, Matthieu, Szarc vel Szic, Katarzyna, Van Der Plas, Ellen, Koppen, Gudrun, Remy, Sylvie, Van Camp, Guy, Lambrechts, Diether, Schoeters, Greet, Vanden Berghe, Wim, De Boever, Patrick |
| المصدر: |
Spanish Journal of Environmental Mutagenesis and Genomics; Vol. 23 No. 1 (2017): XXIII Meeting of the Spanish Environmental Mutagenesis and Genomics Society; 13-14 ; Spanish Journal of Environmental Mutagenesis and Genomics; Vol. 23 Núm. 1 (2017): XXIII Meeting of the Spanish Environmental Mutagenesis and Genomics Society; 13-14 ; 2952-1742 |
| بيانات النشر: |
Spanish Environmental Mutagenesis and Genomics Society (SEMA) |
| سنة النشر: |
2023 |
| المجموعة: |
diffundit - Open Journal Systems Platform |
| الوصف: |
Epigenetic DNA methylation changes can be part of the underlying molecular mechanisms leading to complex diseases. Early life exposures like parental lifestyle and exposure to chemicals can alter DNA methylation patterns, and thereby predispose the child to develop respiratory allergy (RA) later in life. Longitudinal birth cohorts are instrumental to study disease development, but DNA biomarker research is hampered because blood sampling is kept to a minimum for practical and ethical reasons. Saliva is a non-invasive and convenient source of DNA that can be used for biomarker research. In this study, we aimed at discovery and confirmation of differential methylation regions (DMR) in saliva of children with RA when comparing to controls. Saliva samples collected in the two independent longitudinal birth cohorts (Flanders Environment and Health Surveys FLEHS1 & FLEHS2) were analysed using Illumina Methylation 450K BeadChips. A statistical analysis pipeline was developed in R to identify genome-wide differential methylation. We identified 27 DMRs in saliva from 11y old allergic children (self-reported/doctor’s diagnosed RA, Phadiatop IgE ! 0.35 kU/L; N=26) vs. controls (no self-reported/diagnosed RA, Phadiatop IgE< 0.35 kU/L; N=20) in the FLEHS1 cohort. A set of 8 DMRs was selected for further validation by iPLEX MassArray analysis. First, iPLEX analysis was performed in the same 46 FLEHS1 samples that were previously analysed on the 450K methylation arrays, to allow technical validation. iPLEX results correlated significantly with the 450K methylation array data (P<0.0001), though iPLEX analysis confirmed 5 of the 8 identified DMRs in the FLEHS1 study. Aiming for biological confirmation, we studied these DMRs in an independent birth cohort FLEHS2. Due to a lack of blood samples to measure IgE levels in the FLEHS2 cohort, cases and controls were identified as: 1) cases = doctor’s diagnosed/self-reported RA symptoms ever (N=19); and 2) controls = no self-reported/diagnosed RA (N=20). When studying the 8 ... |
| نوع الوثيقة: |
article in journal/newspaper |
| وصف الملف: |
application/pdf |
| اللغة: |
English |
| العلاقة: |
https://ojs.diffundit.com/index.php/sema/article/view/1524/1467Test; https://ojs.diffundit.com/index.php/sema/article/view/1524Test |
| الإتاحة: |
https://ojs.diffundit.com/index.php/sema/article/view/1524Test |
| حقوق: |
Derechos de autor 2023 Spanish Journal of Environmental Mutagenesis and Genomics ; https://creativecommons.org/licenses/by-nc/4.0Test |
| رقم الانضمام: |
edsbas.8F409DDD |
| قاعدة البيانات: |
BASE |
