دورية أكاديمية
Increasing prevalence of non-clade BHIV-1 strains in heterosexual men and women, as monitored by analysis of reverse transcriptase and protease sequences
| العنوان: | Increasing prevalence of non-clade BHIV-1 strains in heterosexual men and women, as monitored by analysis of reverse transcriptase and protease sequences |
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| المؤلفون: | Balotta, C, Facchi, G, Violin, M, Van Dooren, S, Cozzi-Lepri, Alessandro, Forbici, F, Bertoli, A, Riva, C, Senese, D, Caramello, P, Carnevale, G, Rizzardini, G, Cremonini, L, Monno, L, Rezza, G, Perno, CF, Ippolito, G, d'Arminio-Monforte, A, Vandamme, AM., Moroni, M |
| المساهمون: | UCL |
| المصدر: | JAIDS, Vol. 27, no. 5, p. 499-505 (2001) |
| بيانات النشر: | Lippincott Williams & Wilkins |
| سنة النشر: | 2001 |
| المجموعة: | DIAL@UCL (Université catholique de Louvain) |
| مصطلحات موضوعية: | HIV-1 subtypes, circulating HIV-1 recombinant forms, HIV-1 pol sequences, Italian Cohort Naive for Antiretrovirals |
| الوصف: | Objective: We evaluated the prevalence of HIV-1 non-clade B over time in a formerly clade B-restricted area. Protease and reverse transcriptase regions of the pot gene were used for phylogenetic and recombination analysis and for clade assignment to HIV-1 A-D, F-H, J, and K strains of the M group. Methods: The pot gene of 349 HIV-1 patients belonging to the Italian Cohort Naive for Antiretrovirals (ICONA) were genotypically analyzed to study the prevalence of antiretroviral-associated resistance mutations. All HIV-1 pot sequences and 32 HIV reference strains were analyzed, including the reference strains for the major HIV-1 subtypes. The non-clade B sequences according to the HIV-1 Subtyping Tool program were further studied by a bootscan analysis (SimPlot) to investigate the likelihood of recombination between subtypes. Results: Phylogenetic analysis detected 19 of 349 (5.4%) non-clade B subtypes. The proportions of patients carrying non-clade B virus before and after 1997 were 1.9% and 8.4%, respectively (p = .008). Among whites, heterosexual infection and female gender were significantly associated with the presence of non-clade B subtypes (p = .001 and .005, respectively). Non-clade B HIV-1 was harbored by 14.5% of the heterosexuals who were found to be HIV-1 positive after 1997, 60% of whom were women. Bootscan analysis identified four strains as F, two as A, one as C, one as G, and 11 (57.9 %) as non-clade B recombinant subtypes. Conclusion: Detection of HIV-1 subtypes and intersubtype recombinants in a previously clade B-homogeneous area indicates that the HIV-1 epidemic is evolving in Italy and that heterosexuals and women are at increased risk of infection with non-clade B HIV-1 subtypes. Sequences inferred from the pot gene yield to establish the subtype of circulating HIV-1 strains. As a consequence, genotyping of pot gene for testing resistance to antiretrovirals warrants concomitant surveillance of non-clade B subtypes. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1525-4135 1944-7884 |
| العلاقة: | boreal:42553; http://hdl.handle.net/2078.1/42553Test; urn:ISSN:1525-4135; urn:EISSN:1944-7884 |
| DOI: | 10.1097/00126334-200108150-00012 |
| الإتاحة: | https://doi.org/10.1097/00126334-200108150-00012Test http://hdl.handle.net/2078.1/42553Test |
| رقم الانضمام: | edsbas.D9C1A599 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 15254135 19447884 |
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| DOI: | 10.1097/00126334-200108150-00012 |