دورية أكاديمية
Part 2: influence of 2-euryfuryl-1,4-naphthoquinone and its peri-hydroxy derivatives on both cell death and metabolism of TLT cells, a murine hepatoma cell line. modulation of cytotoxicity by vitamin C.
| العنوان: | Part 2: influence of 2-euryfuryl-1,4-naphthoquinone and its peri-hydroxy derivatives on both cell death and metabolism of TLT cells, a murine hepatoma cell line. modulation of cytotoxicity by vitamin C. |
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| المؤلفون: | Benites, Julio, Valderrama, Jaime Adolfo, Taper, Henryk, Buc Calderon, Pedro |
| المساهمون: | UCL - MD/FARM - Ecole de pharmacie |
| المصدر: | Chemical & Pharmaceutical Bulletin, Vol. 57, no. 6, p. 615-619 (2009) |
| بيانات النشر: | Pharmaceutical Society of Japan |
| سنة النشر: | 2009 |
| المجموعة: | DIAL@UCL (Université catholique de Louvain) |
| مصطلحات موضوعية: | Adenosine Triphosphate - metabolism, Animals, Glutathione - metabolism, Hydrogen Bonding, L-Lactate Dehydrogenase - metabolism, Liver Neoplasms, Experimental - drug therapy, metabolism, Mice, Naphthoquinones - chemical synthesis, pharmacology, Neoplasm Transplantation, Antineoplastic Agents - chemical synthesis, Antioxidants - pharmacology, Ascorbic Acid - pharmacology, Caspase 3 - metabolism, Cell Death - drug effects, Cell Line, Tumor, Electrochemistry, Free Radicals - chemistry, Cancer, Cell death, Naphtoquinone, Oxidative stress, Vitamin C |
| الوصف: | 2-Euryfuryl-1,4-naphthoquinone C(1) and its 5- and 5,8-hydroxy derivatives C(2) and C(3), were tested for their cytotoxicity towards transplantable liver tumor (TLT) cells (a murine hepatoma cell line) in the absence and in the presence of vitamin C. Cell death, caspase-3 activity and two metabolic end-points, namely the intracellular content of ATP and glutathione (GSH), were employed to evaluate their cytotoxicity. In a range of concentration from 0 to 10 microg/ml C(1) and C(3) were non toxic against TLT cells, while compound C(2) killed about 50% of cells by necrosis. Interestingly, the presence of vitamin C did not enhance the cytolysis of C(2), but its addition exacerbated the effects of the three compounds on both ATP and GSH contents, the two metabolic end points selected in our study. Our assumption is that the electron donor effect of the peri-hydroxyl substituents on euryfurylnaphthoquinones and the hydrogen bond between the peri-hydroxy and quinone carbonyl groups influence the electron-acceptor capability of the quinone nucleus and thus modifies the electron transfer from ascorbate to the electroactive quinone nucleus. The combination of euryfurylnaphthoquinones with vitamin C may be of potential clinical interest, because cancer cells accumulate vitamin C, they are sensitive to an oxidant insult and they depend on glycolysis (ATP formation) for their survival. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0009-2363 1347-5223 |
| العلاقة: | boreal:106610; http://hdl.handle.net/2078.1/106610Test; info:pmid/19483346; urn:ISSN:0009-2363; urn:EISSN:1347-5223 |
| DOI: | 10.1248/cpb.57.615 |
| الإتاحة: | https://doi.org/10.1248/cpb.57.615Test http://hdl.handle.net/2078.1/106610Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess |
| رقم الانضمام: | edsbas.75198482 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00092363 13475223 |
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| DOI: | 10.1248/cpb.57.615 |