دورية أكاديمية
OATP1B3 expression is strongly associated with Wnt/β-catenin signalling and represents the transporter of gadoxetic acid in hepatocellular carcinoma.
| العنوان: | OATP1B3 expression is strongly associated with Wnt/β-catenin signalling and represents the transporter of gadoxetic acid in hepatocellular carcinoma. |
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| المؤلفون: | Ueno, Akihisa, Masugi, Yohei, Yamazaki, Ken, Komuta, Mina, Effendi, Kathryn, Tanami, Yutaka, Tsujikawa, Hanako, Tanimoto, Akihiro, Okuda, Shigeo, Itano, Osamu, Kitagawa, Yuko, Kuribayashi, Sachio, Sakamoto, Michiie |
| المساهمون: | UCL - (SLuc) Service d'anatomie pathologique, UCL - SSS/IREC/GAEN - Pôle d'Hépato-gastro-entérologie |
| المصدر: | Journal of Hepatology, Vol. 61, no. 5, p. 1080-1087 (2014) |
| بيانات النشر: | Elsevier BV |
| سنة النشر: | 2014 |
| المجموعة: | DIAL@UCL (Université catholique de Louvain) |
| مصطلحات موضوعية: | Adult, Humans, Liver Neoplasms, Magnetic Resonance Imaging, Male, Middle Aged, Organic Anion Transporters, Sodium-Independent, RNA, Messenger, Neoplasm, Wnt Signaling Pathway, beta Catenin, Aged, 80 and over, Carcinoma, Hepatocellular, Cell Line, Tumor, Contrast Media, Female, Gadolinium DTPA, Gene Expression |
| الوصف: | BACKGROUND & AIMS: In the current era of emerging molecular targeted drugs, it is necessary to identify before treatment the specific subclass to which a tumour belongs. Gadoxetic acid is a liver-specific contrast agent that is preferentially taken up by hepatocytes. Therefore, gadoxetic acid-enhanced magnetic resonance imaging (EOB-MRI) should provide precise molecular information about hepatocellular carcinomas (HCCs). The aim of this study was to investigate the transporters of gadoxetic acid in HCC comprehensively and to analyse the molecular regulatory mechanism of such transporters. METHODS : Expression levels of transporters, transcriptional factors and Wnt target genes in clinical samples were examined by quantitative real-time reverse transcription polymerase chain reaction and immunohistochemistry. LiCl treatment of the HCC cell line KYN-2 was conducted in vitro to assess the effects of Wnt signalling activity. RESULTS: Comprehensive analyses of transporter mRNAs and protein expressions revealed that the organic anion transporting polypeptide 1B3 (OATP1B3) had the strongest correlation with tumour enhancement in hepatobiliary-phase images of EOB-MRI. Association analysis with OATP1B3 expression revealed significant correlation with the expression of Wnt/β-catenin target genes. Further, LiCl treatment induced OATP1B3 mRNA expression in KYN-2 cells, indicating a strong association between OATP1B3 expression and Wnt/β-catenin signalling. The sensitivity and specificity to predict Wnt/β-catenin-activated HCC using tumour enhancement in EOB-MRI were 78.9% and 81.7%, respectively. CONCLUSIONS: OATP1B3 was confirmed as the most important transporter mediating HCC enhancement in EOB-MRI. OATP1B3 expression showed a strong association with the expression of Wnt/β-catenin target genes, therefore, OATP1B3-upregulated HCC likely represents a specific subclass of Wnt/β-catenin-activated HCC. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0168-8278 1600-0641 |
| العلاقة: | boreal:165084; http://hdl.handle.net/2078.1/165084Test; info:pmid/24946283; urn:ISSN:0168-8278; urn:EISSN:1600-0641 |
| DOI: | 10.1016/j.jhep.2014.06.008 |
| الإتاحة: | https://doi.org/10.1016/j.jhep.2014.06.008Test http://hdl.handle.net/2078.1/165084Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.3ACEDD2E |
| قاعدة البيانات: | BASE |
| تدمد: | 01688278 16000641 |
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| DOI: | 10.1016/j.jhep.2014.06.008 |