دورية أكاديمية
Cytotoxicity and inhibition of intercellular interaction in N2a neurospheroids by perfluorooctanoic acid and perfluorooctanesulfonic acid
| العنوان: | Cytotoxicity and inhibition of intercellular interaction in N2a neurospheroids by perfluorooctanoic acid and perfluorooctanesulfonic acid |
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| المؤلفون: | Choi, Seong-Kyoon, Kim, Jung-Hee, Park, Jin-Kyu, Lee, Kyeong-Min, Kim, Eunjoo, Jeon, Won Bae |
| بيانات النشر: | Elsevier Ltd |
| سنة النشر: | 2013 |
| المجموعة: | DGIST Scholar (Daegu Gyeongbuk Institute of Science & Technology) |
| مصطلحات موضوعية: | Perfluorinated compounds, Neurospheroids, Intercellular communication, Differentiation markers, Caspase, Reactive oxygen species, RODENT PEROXISOME PROLIFERATOR, PERFLUORINATED FATTY-ACIDS, HEPATOMA HEPG2 CELLS, IN-VITRO, DEVELOPMENTAL NEUROTOXICITY, OXIDATIVE STRESS, PRIMARY CULTURES, NEURONAL CELLS, RAT-LIVER, SULFONATE |
| الوصف: | Effects of perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS) on the neuronal lineage marker expression, cell-cell interaction, caspase-3 mRNA transcription and reactive oxygen species production by N2a neuronal cells were assesses in 3-dimensional (3D) spheroid cultures, and the cytotoxicity were thoroughly compared with those of a conventional 2D monolayer-based toxicity assay. Increasing concentrations of PFOA or PFOS resulted in an increase in cell death. The half maximal inhibitory concentrations measured with spheroids were approximately one and a half times greater than the respective values for monolayer cells. Necrosis was prevalent in spheroids regardless of the dose, whereas the major injury mechanism in monolayers was dependent on compound concentration. Both PFOA and PFOS inhibited neuronal, astrocyte and oligodendrocyte marker gene expression by monolayers and spheroids grown under undifferentiated and all-. trans-retinoic acid-induced differentiating conditions. In the presence of PFOA or PFOS, expression levels of E-cadherin and connexin-43 mRNAs were significantly downregulated, and spheroids were dissociated into single cell populations, indicating that the compounds affect the synthesis of E-cadherin and connexin-43 at the transcriptional level. Results from 3D cultures may provide an insight into potential inhibitory mode of action on gap junctional intercellular communication. © 2013 Elsevier Ltd. ; FALSE |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | unknown |
| تدمد: | 0278-6915 |
| العلاقة: | http://hdl.handle.net/20.500.11750/3199Test; 2-s2.0-84883437850; Food and Chemical Toxicology, v.60, pp.520 - 529 |
| DOI: | 10.1016/j.fct.2013.07.070 |
| الإتاحة: | https://doi.org/20.500.11750/3199Test https://doi.org/10.1016/j.fct.2013.07.070Test https://hdl.handle.net/20.500.11750/3199Test |
| رقم الانضمام: | edsbas.4EB119FF |
| قاعدة البيانات: | BASE |
| تدمد: | 02786915 |
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| DOI: | 10.1016/j.fct.2013.07.070 |