دورية أكاديمية
Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats
| العنوان: | Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats |
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| المؤلفون: | NAVANUKRAW, Chainarong, THAMMASIRI, Jiratti, MOONMANEE, Tossapol, NATTHAKORNKUL, Jaruk |
| المصدر: | Volume: 38, Issue: 6 699-706 ; 1300-0128 ; 1303-6181 ; Turkish Journal of Veterinary and Animal Sciences |
| بيانات النشر: | TÜBİTAK TUBITAK |
| سنة النشر: | 2014 |
| المجموعة: | DergiPark Akademik (E-Journals) |
| مصطلحات موضوعية: | Vascular endothelial growth factor,hypoxia-inducible factor-1 alpha,periovulatory period,goats |
| الوصف: | To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1 alpha) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1 alpha were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P < 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1 alpha at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8-24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1 alpha in granulosa at 4 h was not changed but levels were increased (P < 0.05) at 8 h and at 12 h and then decreased at 24 and 48 h, respectively. These data describe the relationships of VEGF- and HIF-1 alpha -dependent angiogenesis during the periovulatory periods by which hypoxia during ovulation is crucial for establishing the thecal new vasculature. ; To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1 alpha) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1 alpha were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P < 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1 alpha at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8-24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1 ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Turkish |
| العلاقة: | https://dergipark.org.tr/tr/download/article-file/134150Test; https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/12594/152742Test |
| DOI: | 10.3906/vet-1404-54 |
| الإتاحة: | https://doi.org/10.3906/vet-1404-54Test https://dergipark.org.tr/tr/pub/tbtkveterinary/issue/12594/152742Test |
| رقم الانضمام: | edsbas.63918A8F |
| قاعدة البيانات: | BASE |
| DOI: | 10.3906/vet-1404-54 |
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