دورية أكاديمية
Use of in vitro propagation of `Obla?inska? sour cherry in rootstock breeding
| العنوان: | Use of in vitro propagation of `Obla?inska? sour cherry in rootstock breeding |
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| المؤلفون: | DORIC, DUSICA, OGNJANOV, VLADISLAV, BARAC, GORAN, LJUBOJEVIC, MIRJANA, PRANJIC, ANKICA, DUGALIC, KRUNOSLAV, ERCİŞLİ, SEZAİ |
| المصدر: | Volume: 39, Issue: 4 575-581 ; 1300-0152 ; 1303-6092 ; Turkish Journal of Biology |
| بيانات النشر: | TÜBİTAK TUBITAK |
| سنة النشر: | 2015 |
| المجموعة: | DergiPark Akademik (E-Journals) |
| مصطلحات موضوعية: | Dormant period,elongation,explant collection date,Prunus cerasus L.,rooting |
| الوصف: | Prunus cerasus L. `Obla?inska? sour cherry germplasm was established in vitrodirectly from in situ plants on different explant collection dates, enabling quick clonal multiplication and introduction to a rootstock breeding program. Rosette initiation of four investigated genotypes was possible from November to April on the medium containing Schenk and Hildebrandt (SH) macroelements, Murashige and Skoog (MS) microelements, and vitamins supplemented with (in mg L?1) 6-benzyladenine (BA), 0.5; indole-3-butyric acid (IBA), 0.01; gibberellic acid (GA3), 0.1; citric acid, 10; and L-ascorbic acid, 10. The lowest percent of contamination was noted in November and December, when dormant buds were used as an explant source, and the highest percentage was found with actively growing shoot tips. The elongation phase of rosettes initiated from dormant buds remained a major obstacle. Survival rates of shoot tips obtained in April were high and subsequent growth more prominent. An increasing index of multiplication from 1.5 to 1.9 was noted on Driver and Kuniyuki walnut medium (DKW) with 0.8 mg L?1 BA and 0.01 mg L?1 IBA in the `OV 32? genotype. Rooting percentages of 71.3% and 81.3% were achieved in `OV 17? and `OV 32? genotypes, respectively, on half-strength MS medium with 1 mg L?1 IBA. ; Prunus cerasus L. `Obla?inska? sour cherry germplasm was established in vitrodirectly from in situ plants on different explant collection dates, enabling quick clonal multiplication and introduction to a rootstock breeding program. Rosette initiation of four investigated genotypes was possible from November to April on the medium containing Schenk and Hildebrandt (SH) macroelements, Murashige and Skoog (MS) microelements, and vitamins supplemented with (in mg L?1) 6-benzyladenine (BA), 0.5; indole-3-butyric acid (IBA), 0.01; gibberellic acid (GA3), 0.1; citric acid, 10; and L-ascorbic acid, 10. The lowest percent of contamination was noted in November and December, when dormant buds were used as an explant source, and the highest ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | Turkish |
| العلاقة: | https://dergipark.org.tr/tr/download/article-file/121973Test; https://dergipark.org.tr/tr/pub/tbtkbiology/issue/11762/140549Test |
| DOI: | 10.3906/biy-1412-85 |
| الإتاحة: | https://doi.org/10.3906/biy-1412-85Test https://dergipark.org.tr/tr/pub/tbtkbiology/issue/11762/140549Test |
| رقم الانضمام: | edsbas.60AE6652 |
| قاعدة البيانات: | BASE |
| DOI: | 10.3906/biy-1412-85 |
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