التفاصيل البيبلوغرافية
| العنوان: |
IL-10 suppressor activity and ex vivo Tr1 cell function are impaired in multiple sclerosis |
| المؤلفون: |
Martinez-Forero, I. (Iván), Garcia-Muñoz, R. (R.), Martinez-Pasamar, S. (Sara), Inoges, S. (Susana), Lopez-Diaz-de-Cerio, A. (Ascensión), Palacios, R. (Ricardo), Sepulcre, J. (Jorge), Moreno, B. (Beatriz), Gonzalez, Z. (Zaira), Fernandez-Diez, B. (B.), Melero, I. (Ignacio), Bendandi, M. (Maurizio), Villoslada, P. (Pablo) |
| بيانات النشر: |
Wiley-Blackwell |
| سنة النشر: |
2008 |
| المجموعة: |
dadun - Depósito Académico Digital Universidad de Navarra |
| مصطلحات موضوعية: |
CD46, IL-10, MS, Stat-3, Tr1 cells Abbreviations: c(t): cycle threshold HC: healthy control MNE: mean normalized expression MS: multiple sclerosis Socs: suppressor of cytokine signaling Tr1 cell: T regulatory cell type 1 576 Ivan Martinez-Forero et al. Eur. J. Immunol. 2008. 38: 576–586 f 2008 |
| الوصف: |
T regulatory cells type 1 (Tr1 cells) are excellent candidates for cell therapy in multiple sclerosis (MS). The aim of our study was to assess the functional state of Tr1 cells and IL-10R signaling in patients with MS. Tr1 cells were induced in vitro by activation with anti-CD46 antibodies in controls and patients with MS. Cells were phenotyped by cytometry and suppression assays, and the expression of cytokines and transcription factors was evaluated by real-time PCR, ELISA, cytometry and Western blotting. We found that the activity of Tr1 cells and IL-10R signaling is impaired in MS patients since Tr1 cells isolated from MS patients produced less IL-10 than those obtained from controls. Indeed, the supernatants from Tr1 cells from controls did not suppress the proliferation of stimulated CD4(+) cells from patients with MS. Furthermore, the IL-10R signaling pathway was not fully active in CD4(+) cells from MS patients and these cells had higher baseline levels of SOCS3 transcripts than controls. Indeed, after in vitro IL-10 stimulation, the expression levels of the STAT1, STAT3 and IL-10RA genes were higher in MS patients than in controls. Moreover, Stat-3 phosphorylation was lower in controls than in patients after IL-10 stimulation. These results indicate that IL-10 regulatory function is impaired in patients with MS. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| العلاقة: |
https://hdl.handle.net/10171/18714Test |
| الإتاحة: |
https://hdl.handle.net/10171/18714Test |
| حقوق: |
info:eu-repo/semantics/closedAccess |
| رقم الانضمام: |
edsbas.39EF3FCD |
| قاعدة البيانات: |
BASE |
