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1دورية أكاديمية
المؤلفون: Rodríguez-Pena, A. (Alejandro), Armendáriz, E. (Estibaliz), Oyarbide, A. (Alvaro), Morales-Urteaga, X. (Xabier), Ortiz-Espinosa, S. (Sergio), Ruiz-Fernandez-de-Cordoba, B. (Borja), Cochonneau, D. (Denis), Cornago, I. (Iñaki), Heymann, D. (Dominique), Argemí, J. (Josepmaria), D'Avola, D. (Delia), Sangro, B. (Bruno), Lecanda, F. (Fernando), Pio, R. (Rubén), Cortés-Dominguez, I. (Iván), Ortiz-de-Solorzano, C. (Carlos)
مصطلحات موضوعية: Cell enrichment, Cell sorting, Circulating tumor cells, CTC, Inertial forces, Liquid biopsy
الوصف: The analysis of circulating tumor cells (CTCs) in blood is a powerful noninvasive alternative to conventional tumor biopsy. Inertial-based separation is a promising high-throughput, marker-free sorting strategy for the enrichment and isolation of CTCs. Here, we present and validate a double spiral microfluidic device that efficiently isolates CTCs with a fine-tunable cut-off value of 9 mu m and a separation range of 2 mu m. We designed the device based on computer simulations that introduce a novel, customized inertial force term, and provide practical fabrication guidelines. We validated the device using calibration beads, which allowed us to refine the simulations and redesign the device. Then we validated the redesigned device using blood samples and a murine model of metastatic breast cancer. Finally, as a proof of principle, we tested the device using peripheral blood from a patient with hepatocellular carcinoma, isolating more than 17 CTCs/ml, with purity/removal values of 96.03% and 99.99% of white blood cell and red blood cells, respectively. These results confirm highly efficient CTC isolation with a stringent cut-off value and better separation results than the state of the art.
وصف الملف: application/pdf
العلاقة: info:eu-repo/grantAgreement/ISCIII/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII)/PI20%2F00419/ES/MODULACION DEL SISTEMA DEL COMPLEMENTO PARA MEJORAR LA ACTIVIDAD ANTITUMORAL Y DISMINUIR LOS EFECTOS SECUNDARIOS DE LA INMUNOTERAPIA FRENTE AL CANCER DE PULMON; info:eu-repo/grantAgreement/MINECO/Proyectos de I+D+I dentro del Programa Estatal Retos de la Sociedad (2015)/DPI2015-64221-C2-1/ES/DISEÑO Y FABRICACION DE DISPOSITIVOS MICROFLUIDICOS PARA EL ESTUDIO MEDIANTE ANALISIS DE IMAGEN Y MODELOS NUMERICOS DE LA MECANICA DE LA EXTRAVASACION TUMORAL; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094494-B-C22/ES/DISEÑO DE HERRAMIENTAS DE MICROSCOPIA TRIDIMENSIONAL Y PROCESADO DE IMAGEN PARA EL ANALISIS AUTOMATIZADO DEL CRECIMIENTO DE ORGANOIDES TUMORALES; https://hdl.handle.net/10171/65295Test
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2دورية أكاديمية
المؤلفون: Grünewald, T.G.P. (Thomas G. P.), Alonso-Roldán, M.M. (Marta María), Avnet, S. (Sofia), Banito, A. (Ana), Burdach, S. (Stefan), Cidre-Aranaz, F. (Florencia), Di-Pompo, G. (Gemma), Distel, M. (Martin), Dorado-Garcia, H. (Heathcliff), Garcia-Castro, J. (Javier), González-González, L. (Laura), Grigoriadis, A.E. (Agamemnon), Kasan, M. (Merve), Koelsche, C. (Christian), Krumbholz, M. (Manuela), Lecanda, F. (Fernando), Lemma, S. (Silvia), Longo, D.L. (Dario L.), Madrigal-Esquivel, C. (Claudia), Morales-Molina, Á. (Álvaro), Musa, J. (Julian), Ohmura, S. (Shunya), Ory, B. (Benjamin), Pereira-Silva, M. (Miguel), Perut, F. (Francesca), Rodríguez, R. (René), Seeling, C. (Carolin), Al-Shaaili, N. (Nada), Shaabani, S. (Shabnam), Shiavone, K. (Kristina), Sinha, S. (Snehadri), Tomazou, E.M. (Eleni M.), Trautmann, M. (Marcel), Vela, M. (Maria), Versleijen-Jonkers, Y.M.H. (Yvonne M. H.), Visgauss, J. (Julia), Zalacain, M. (Marta), Schober, S.J. (Sebastian J.), Lissat, A. (Andrej), English, W.R. (William R.), Baldini, N. (Nicola), Heymann, D. (Dominique)
مصطلحات موضوعية: Cancer stem cells, Cell dormancy, Chorioallantoic membrane (CAM) models, Circulating tumor cells (CTCs), Epigenomic alterations, Extracellular vesicles (EVs), Genomic alterations, Immunotherapy, Oncolytic viruses, Orthotopic xenografts, Patient-derived xenografts (PDXs), Pediatric tumors, Precision medicine, Sarcomas, Tumor microenvironment (TME)
الوصف: Sarcomas are heterogeneous and clinically challenging soft tissue and bone cancers. Although constituting only 1% of all human malignancies, sarcomas represent the second most common type of solid tumors in children and adolescents and comprise an important group of secondary malignancies. More than 100 histological subtypes have been characterized to date, and many more are being discovered due to molecular profiling. Owing to their mostly aggressive biological behavior, relative rarity, and occurrence at virtually every anatomical site, many sarcoma subtypes are in particular difficult-to-treat categories. Current multimodal treatment concepts combine surgery, polychemotherapy (with/without local hyperthermia), irradiation, immunotherapy, and/or targeted therapeutics. Recent scientific advancements have enabled a more precise molecular characterization of sarcoma subtypes and revealed novel therapeutic targets and prognostic/predictive biomarkers. This review aims at providing a comprehensive overview of the latest advances in the molecular biology of sarcomas and their effects on clinical oncology; it is meant for a broad readership ranging from novices to experts in the field of sarcoma.
وصف الملف: application/pdf
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3دورية أكاديمية
مصطلحات موضوعية: Materias Investigacion::Ciencias de la Salud::Hematología, Flow cytometry, Minimal residual disease, Circulating tumor cells, Survival
الوصف: Purpose of review: Although the input of multiparameter flow cytometry (MFC) into the clinical management of multiple myeloma (MM) patients has faced some reluctance, continuously growing evidence supports the utility of MFC in this disease. Recent findings: MFC immunophenotyping of bone marrow and peripheral blood plasma cells affords cost-effective assessment of clonality, and provides prognostic information on the risk of progression in smoldering MM, and the identification of active MM patients with dismal outcome (e.g.: high numbers of circulating tumor cells) or long-term survival despite sub-optimal responses through the characterization of MGUS-like phenotypes. Extensive data indicates that MRD monitoring can be used as biomarker to evaluate treatment efficacy and act as surrogate for survival. The time has come to address within clinical trials, the exact role of baseline risk factors and MRD monitoring for tailored therapy in MM, which implies systematic usage of highly sensitive cost-effective, readily available and standardized MRD techniques such as MFC. Summary: Next-generation MFC should be considered mandatory in the routine evaluation of MM patients both at diagnosis and after therapy, and represents an attractive technique to integrate with high-throughput DNA and RNA-seq methods to help understanding the mechanisms behind dissemination and chemoresistance of MM.
وصف الملف: application/pdf
العلاقة: https://journals.lww.com/co-oncology/Abstract/2016/11000/Utility_of_flow_cytometry_studies_in_the.9.aspxTest; info:eu-repo/grantAgreement/EC/H2020/680200/EU; https://hdl.handle.net/10171/52184Test
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4دورية أكاديمية
المؤلفون: Gutierrez, C. (Cristina), Rodriguez, J. (Javier), Patiño-García, A. (Ana), Garcia-Foncillas, J. (Jesús), Salgado, J. (Josefa)
مصطلحات موضوعية: KRAS gene, Circulating tumor cells, Inmunomagnetic labeling, Metastatic colorectal cancer, Peripheral blood
الوصف: The present study describes an optimized method for isolating peripheral blood circulating tumor cells (CTCs) and performing KRAS mutation analysis. The approach combines isolation of peripheral blood mononuclear cells and immunomagnetic labeling with CD45 and CD326 human microbeads with KRAS analysis performed with a Therascreen KRAS kit by quantitative PCR. KRAS mutations were detected in the CTCs of patients with metastatic colorectal cancer (mCRC). CTCs may represent an alternative to invasive procedures and their analysis may be representative of the current disease status of the patient. This proposed analysis may be performed in a daily clinical practice.
وصف الملف: application/pdf
