رسالة جامعية
Fatty acid elongation in methylotrophic yeast Hansenula polymorpha CBS 1976 and fatty acid auxotrophic mutants ; กระบวนการต่อสายยาวของกรดไขมันในเมทิลโลโทรฟิกยีสต์ Hansenula polymorpha CBS 1976 และสายพันธุ์กลายชนิดที่ต้องการกรดไขมัน
| العنوان: | Fatty acid elongation in methylotrophic yeast Hansenula polymorpha CBS 1976 and fatty acid auxotrophic mutants ; กระบวนการต่อสายยาวของกรดไขมันในเมทิลโลโทรฟิกยีสต์ Hansenula polymorpha CBS 1976 และสายพันธุ์กลายชนิดที่ต้องการกรดไขมัน |
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| المؤلفون: | Wanida Wongsumpanchai, 1972- |
| المساهمون: | Amorn Petsom, Sarintip Anamnart, Chulalongkorn University. Faculty of Science |
| بيانات النشر: | Chulalongkorn University |
| سنة النشر: | 2004 |
| المجموعة: | CUIR - Chulalongkorn University Intellectual Repository |
| مصطلحات موضوعية: | Fatty acids, Elongation, Methylotrophic yeast, Auxotrophic mutants, Hansenula polymorpha |
| الوصف: | Thesis (Ph.D.)--Chulalongkorn University, 2004 ; Fatty acid auxotrophic mutants (V1 to V10) of Hansenula polymorpha were isolated from the wild-type strain (leu1-1) of H. polymorpha CBS 1976 by mutagenization with ethyl methanesulfonate (EMS). All the mutants were screened as colonies which were unable to proliferate on YEPD after replication from initial YEPD containing a mixture of 1mM each of C14:0 C16:0 and C18:0 where the mutagenized cells were plated. In order to classify the mutants, their growth on media supplemented with various fatty acids of 1mM and/or 2mM C14:0 C16:0 and C18:0 were examined. Three groups of mutants possessing different phenotype were identified. The first group including six mutants (V1 to V6) did not grow on solid media supplemented with 1mM of C16:0, however, 2mM of C16:0 and 1-2mM of C18:0 could support the growth of this group. Ability of the first group which were unable to grow in 1mM C16:0 suggesting that this group of the mutants might have partially defect at the step of elongation of C16 to C18. The second group included only one mutant (V7) whose no growth when grown on media supplemented with 1mM of C14:0 and when cultivated on media supplemented with a mixture of C14:0 C16:0 and C18:0 noted that C14:0 was also increased when compared with those of the parental strain in the same media. Taken together, from these results suggesting that this mutant probably have the defect at the elongation of C14:0 to C16:0. Mutants in the third group (V8 V9 and V10) did not grow on medium cultivated with 1mM of C16:0 and 1mM of C18:0 supplementation but demonstrated their ability to proliferate on media with addition of C14:0 suggesting that this mutant might have the defect at the elongation before C14. From crosses of all the three groups of the mutants with an auxotrophic strain (ura3-1), all diploid hybrids were selected on minimal medium containing a mixture of C14:0, C16:0 and C18:0 fatty acids without uracil and MIN with uracil. The segregants of all the three groups were chosen ... |
| نوع الوثيقة: | thesis |
| وصف الملف: | 2577917 bytes; application/pdf |
| اللغة: | English |
| ردمك: | 978-974-17-5913-2 974-17-5913-4 |
| العلاقة: | http://cuir.car.chula.ac.th/handle/123456789/4062Test |
| الإتاحة: | http://cuir.car.chula.ac.th/handle/123456789/4062Test |
| حقوق: | Chulalongkorn University |
| رقم الانضمام: | edsbas.88253188 |
| قاعدة البيانات: | BASE |
| ردمك: | 9789741759132 9741759134 |
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