دورية أكاديمية
Development of on-line spectrofluorimetric methodology for selenium monitoring in foods and biological fluids using Chrome azurol S quenching
| العنوان: | Development of on-line spectrofluorimetric methodology for selenium monitoring in foods and biological fluids using Chrome azurol S quenching |
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| المؤلفون: | Santarossa, Debora Gimena, Fernandez, Liliana Patricia |
| بيانات النشر: | Elsevier Science |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | SELENIUM, FI-SPECTROFLUOROMETRY, CHROME AZUROL S, SODIUM CHOLATE, https://purl.org/becyt/ford/1.4Test, https://purl.org/becyt/ford/1Test |
| الوصف: | A novel, simple and accurate on-line green methodology for Se(IV) monitoring by molecular fluorescence has been developed. Because the analyte does not exhibit fluorescence, the organic dye Chrome azurol S (CAS) has been chosen to allow detection. The effect of metal-quenching on CAS excitation and emission conditions (λex=300 nm; λem=407 nm) was used as criterion for analyte quantification in presence of sodium cholate bile salt (NaC). The quenching mechanism was explored, and it can be classified as a collisional type with a Stern-Volmer constant value of 3.0×107 mol L−1. To improve the sampling rate, minimize the reagent consumption and generated wastes, an on-line configuration was designed. Experimental variables that affect the fluorimetric sensitivity were optimized using uni-variation assays. Under optimal experimental conditions, the limit of detection was 0.27 µg L−1 with a lineal range for Se(IV) concentration from 0.84 to 6.00 µg L−1. The developed methodology is low cost and fast sampling, allowing Se(IV) quantification in the presence of other common ions. Bulbous vegetables and biological samples were successfully analyzed with an average recovery close to 100%. ; Fil: Santarossa, Debora Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina ; Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0039-9140 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S0039914017305295Test; http://hdl.handle.net/11336/64761Test; Santarossa, Debora Gimena; Fernandez, Liliana Patricia; Development of on-line spectrofluorimetric methodology for selenium monitoring in foods and biological fluids using Chrome azurol S quenching; Elsevier Science; Talanta; 172; 9-9-2017; 31-36; CONICET Digital; CONICET |
| الإتاحة: | https://doi.org/10.1016/j.talanta.2017.05.013Test http://hdl.handle.net/11336/64761Test |
| حقوق: | info:eu-repo/semantics/restrictedAccess ; https://creativecommons.org/licenses/by-nc-sa/2.5/arTest/ |
| رقم الانضمام: | edsbas.EFDC114E |
| قاعدة البيانات: | BASE |
| تدمد: | 00399140 |
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