دورية أكاديمية
Restorative effect of intracerebroventricular insulin-like growth factor-I gene therapy on motor performance in aging rats
| العنوان: | Restorative effect of intracerebroventricular insulin-like growth factor-I gene therapy on motor performance in aging rats |
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| المؤلفون: | Nishida, Fabian, Morel, Gustavo Ramón, Hereñú, Claudia Beatriz, Schwerdt, José Ignacio, Goya, Rodolfo Gustavo, Portiansky, Enrique Leo |
| بيانات النشر: | Pergamon-Elsevier Science Ltd |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | CEREBRAL VENTRICLES, SPINAL CORD, IGF-I, EPENDYMA, QDOTS, https://purl.org/becyt/ford/3.1Test, https://purl.org/becyt/ford/3Test |
| الوصف: | Insulin-like growth factor-I (IGF-I) is a powerful neuroprotective molecule in the brain and spinal cord. We have previously shown that intracerebroventricular (i.c.v.) IGF-I gene therapy is an effective strategy to increase IGF-I levels in the cerebrospinal fluid (CSF). Since aging in rats is associated with severe motor function deterioration, we implemented i.c.v. IGF-I gene therapy in very old rats (30–31 months) and assessed the beneficial impact on motor performance. We used recombinant adenovectors (RAds) expressing either green fluorescent protein (GFP) or rat IGF-I. Injection in the lateral or fourth ventricle led to high transgene expression in the ependymal cell layer in the brain and cervical spinal cord. RAd-IGF-I-injected rats but not RAd-GFP-injected controls, showed significantly increased levels of CSF IGF-I. Motor tests showed the expected age-related decline in aged rats. Seventeen-day IGF-I gene therapy induced a significant improvement in motor performance in the aged but not in the young animals. These results show that IGF-I is an effective restorative molecule in the aging brain and spinal cord. The data also reveal that the ependymal route constitutes a promising approach for implementing protective IGF-I gene therapy in the aging CNS. ; Fil: Nishida, Fabian. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina ; Fil: Morel, Gustavo Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner". Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata "Prof. Dr. Rodolfo R. Brenner"; Argentina ; Fil: Hereñú, Claudia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 0306-4522 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0306452211000364Test; http://hdl.handle.net/11336/108687Test; Nishida, Fabian; Morel, Gustavo Ramón; Hereñú, Claudia Beatriz; Schwerdt, José Ignacio; Goya, Rodolfo Gustavo; et al.; Restorative effect of intracerebroventricular insulin-like growth factor-I gene therapy on motor performance in aging rats; Pergamon-Elsevier Science Ltd; Neuroscience; 177; 17-3-2011; 195-206; CONICET Digital; CONICET |
| الإتاحة: | https://doi.org/10.1016/j.neuroscience.2011.01.013Test http://hdl.handle.net/11336/108687Test |
| حقوق: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by-nc-sa/2.5/arTest/ |
| رقم الانضمام: | edsbas.9A10C763 |
| قاعدة البيانات: | BASE |
| تدمد: | 03064522 |
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