دورية أكاديمية
Effect of Etidronate and Ibandronate on Cytosolic Ca2+ in HT29 and Parasite Cell Line from Echinococcus Granulosus sensu lato
| العنوان: | Effect of Etidronate and Ibandronate on Cytosolic Ca2+ in HT29 and Parasite Cell Line from Echinococcus Granulosus sensu lato |
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| المؤلفون: | Ferrulli, Mariana, Pérez Rojo, Fernando Gabriel, Granada Herrera, Lilian Andrea, Maglioco, Andrea Florencia, Roldán, Emilio A. J., Fuchs, Alicia Graciela |
| بيانات النشر: | Bentham Science Publishers |
| المجموعة: | CONICET Digital (Consejo Nacional de Investigaciones Científicas y Técnicas) |
| مصطلحات موضوعية: | CALCIUM SALTS, CYTOPLASMIC CALCIUM, ECHINOCOCCUS GRANULOSUS, ETIDRONATE, HT29 CELL, IBANDRONATE, https://purl.org/becyt/ford/3.1Test, https://purl.org/becyt/ford/3Test |
| الوصف: | Background: The bisphosphonates are synthetic analogs of pyrophosphate in which two phosphates are connected through carbon instead of oxygen. They are approved compounds for the treatment of hypercalcemia, bone diseases and they have been proposed to treat infectious diseases. Bisphosphonates’ main mechanisms of action are on calcium metabolism, inhibition of protein prenylation and on ATP synthesis. In a previous work, the antiparasitic activity of bisphosphonates on a cell line from Echinococcus granulosus, sensu lato protoscoleces, 30 µM etidronate and ibandronate have antiproliferative activity after 72 h of incubation, decreasing intracellular ATP and only etidronate increased intracellular total calcium concentration. Objective: This work studied the effect of etidronate and ibandronate on cytoplasmic ionic calcium concentration in parasitic cell line and in HT29, cell line from human colon adenocarcinoma. Methods: Ionic calcium was measured by spectrofluorometric, labeling cells with Fluo-4AM. Cells were suspended in Na+ or K+ rich buffer and two calcium salts were used Cl- or Gluc- , anion permeable and impermeable, respectively. Results: Remarkable differences between cell lines were shown with the effect of bisphosphonates on intracellular ionic calcium concentration in hyperpolarized cells and these differences were smoothed on depolarized cells, in spite of the similar cellular response to calcium salts in absence of bisphosphonates. Conclusion: The bisphosphonates, mainly etidronate, decreased intracellular ionic calcium on parasitic cells explaining other aspects of their antiproliferative effect. Results suggested that other mechanism, such as Cl- and Na+ interchange are differentially affected by bisphosphonates, depending on cell line origin ; Fil: Ferrulli, Mariana. Universidad Abierta Interamericana. Secretaría de Investigación. Centro de Altos Estudios En Ciencias Humanas y de la Salud - Sede Buenos Aires; Argentina ; Fil: Pérez Rojo, Fernando Gabriel. Universidad Abierta Interamericana. ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1874-4214 |
| العلاقة: | info:eu-repo/semantics/altIdentifier/url/https://openparasitologyjournal.com/VOLUME/7/PAGE/19Test/; http://hdl.handle.net/11336/133289Test; Ferrulli, Mariana; Pérez Rojo, Fernando Gabriel; Granada Herrera, Lilian Andrea; Maglioco, Andrea Florencia; Roldán, Emilio A. J.; et al.; Effect of Etidronate and Ibandronate on Cytosolic Ca2+ in HT29 and Parasite Cell Line from Echinococcus Granulosus sensu lato; Bentham Science Publishers; The Open Parasitology Journal; 7; 1; 2-2019; 19-25; CONICET Digital; CONICET |
| الإتاحة: | https://doi.org/10.2174/1874421401907010019Test http://hdl.handle.net/11336/133289Test |
| حقوق: | info:eu-repo/semantics/openAccess ; https://creativecommons.org/licenses/by/2.5/arTest/ |
| رقم الانضمام: | edsbas.EA00BF69 |
| قاعدة البيانات: | BASE |
| تدمد: | 18744214 |
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