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1دورية أكاديمية
مصطلحات موضوعية: SENESCENCE, PROTEASES, N REMOBILIZATION, https://purl.org/becyt/ford/4.5Test, https://purl.org/becyt/ford/4Test
الوصف: Senescence is the final developmental stage of every plant organ, which leads to cell death. It is a highly regulated process, involving differential gene expression and outstanding increment in the rate of protein degradation. Senescence-associated proteolysis enables the remobilization of nutrients, such as nitrogen (N), from senescent tissues to developing organs or seeds. In addition to the nutrient recycling function, senescence-associated proteases are also involved in the regulation of the senescence process. Nearly, all protease families have been associated with some aspects of plant senescence, and numerous reports addressing the new identification of senescence-associated proteases are published every year. Here, we provide an updated report with the most recent information published in the field, focusing on senescence-associated proteases presumably involved in N remobilization. ; Fil: Roberts, Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina ; Fil: Caputo Suarez, Carla Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina ; Fil: Criado, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales; Argentina ; Fil: Funk, Christiane. Universidad de Umea; Suecia
وصف الملف: application/pdf
العلاقة: http://hdl.handle.net/11336/98245Test; Roberts, Irma; Caputo Suarez, Carla Paula; Criado, Maria Victoria; Funk, Christiane; Senescence-associated proteases in plants; Wiley Blackwell Publishing, Inc; Physiologia Plantarum; 145; 1; 5-2012; 130-139; CONICET Digital; CONICET
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2دورية أكاديمية
المؤلفون: Roberts, Irma, Veliz, Cintia Gabriela, Criado, Maria Victoria, Signorini, Ana, Simonetti, Ester, Caputo Suarez, Carla Paula
مصطلحات موضوعية: BARLEY (HORDEUM VULGARE L.), NITROGEN REMOBILIZATION, PROTEASES, PROTEOLYSIS, SENESCENCE, SUBTILASES, https://purl.org/becyt/ford/1.6Test, https://purl.org/becyt/ford/1Test
الوصف: Subtilases are one of the largest groups of the serine protease family and are involved in many aspects of plant development including senescence. In wheat, previous reports demonstrate an active participation of two senescence-induced subtilases, denominated P1 and P2, in nitrogen remobilization during whole plant senescence. The aim of the present study was to examine the participation of subtilases in senescence-associated proteolysis of barley leaves while comparing different senescence types. With this purpose, subtilase enzymatic activity, immunodetection with a heterologous antiserum and gene expression of 11 subtilase sequences identified in barley databases by homology to P1 were analyzed in barley leaves undergoing dark-induced or natural senescence at the vegetative or reproductive growth phase. Results showed that subtilase specific activity as well as two inmunoreactive bands representing putative subtilases increased in barley leaves submitted to natural and dark-induced senescence. Gene expression analysis showed that two of the eleven subtilase genes analyzed, HvSBT3 and HvSBT6, were up-regulated in all the senescence conditions tested while HvSBT2 was expressed and up-regulated only during dark-induced senescence. On the other hand, HvSBT1, HvSBT4 and HvSBT7 were down-regulated during senescence and two other subtilase genes (HvSBT10 and HvSBT11) showed no significant changes. The remaining subtilase genes were not detected. Results demonstrate an active participation of subtilases in protein degradation during dark-induced and natural leaf senescence of barley plants both at the vegetative and reproductive stage, and, based on their expression profile, postulate HvSBT3 and HvSBT6 as key components of senescence-associated proteolysis. ; Fil: Roberts, Irma. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones en Biociencias Agrícolas y Ambientales. Universidad de Buenos Aires. Facultad de Agronomía. ...
وصف الملف: application/pdf
العلاقة: info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0176161717300238Test; http://hdl.handle.net/11336/48962Test; Roberts, Irma; Veliz, Cintia Gabriela; Criado, Maria Victoria; Signorini, Ana; Simonetti, Ester; et al.; Identification and expression analysis of 11 subtilase genes during natural and induced senescence of barley plants; Elsevier Gmbh; Journal of Plant Physiology; 211; 4-2017; 70-80; CONICET Digital; CONICET
